1) Assuming that growth rate is expressed with monod kinetics, plot of 1/ U vs 1/C

will be a straight line

1/u_max 5261 u_max 1.90E-04

K_s/u_max 16320 K_s 3.10E+00

2) A) For a single steady-state CSTF with a sterile feed, the dilution rate is equal
to the specific growth rate:

The cell concentration of the outlet stream is

B) For two CSTFs in series, the first fermenter must be operated at CX,opt and
CS,opt therefore,
For the second fermenter,

Therefore total volume is : V=V1+V2=950+192=1142 which is 20% smaller than

volume required when using a single CSTF.
C) The best combination is a CSTF operated at the maximum rate followed by a
PFF. The volume of the first fermenter is 950 L as calculated in part (b).

3) A) The steady-state of cell concentration is increased by a factor of 1/(1 + α –

αC) by cell recycling. Incorporate this factor in the Monod’s equation to
determine the effluent concentration (S) that is desired to be 100 mg/l, thus :

B) If X is the cell concentration, consider X1 to be the cell concentration in the

reactor and Xr to be the cell concentration in the recycle stream. The cell
concentration in the reactor is calculated from the following:
C) The volume of the sedimentation tank is as follow : VST=ST(1+)F=21.6100
=320 l So, the volume of the sedimentation tank is 320 liters
Biomass balance around the sedimentation tank is as follows :
(1+ α)FX1=FX2+FXr
X2=(1+ α)X1- α
Xr=(1.64.9)-0.624.9=1.960 g/l
So, the cell concentration in the sedimentation tank is 1.960 g/l or 1960 mg/l.