Materials and Equipment

Table 1. Materials and Equipment used for the Crude Fat determination.

Materials Quantity
Erlenmeyer flask (250 mL) 2 pcs.
Whatman Filter paper 2 pcs.
Wire gauze 1 pc.
Cork 2 pcs.
Analytical Balance 1 pc.
Ethanol

The Table 3.1 shows the materials and equipment used for the Crude Fat experiment.
Each of the listed materials and equipment has its own function to perform. The erlenmeyer
flask contains the sample. The whatman filter paper will be used to wrap the sample. The wire
gauze will be used when the flask was heated. The cork will be used as a cover for the
erlenmeyer flask so that the sample will not able to inhibit moisture. The analytical balance was
used to determine the weigh of the flask.

Procedure.

a. Preparation of the Sample

The sample used for the crude fat determination is the banana peel patty. It was then
weigh into two separate 5g and were wrapped using filter paper.

b. Soaking of the Sample

After the sample was wrapped, put it into two (2) different flask. Soak the sample into
ethanol for about three (3) days. Cover the flask using the cork.

c. Distillation

After storing the sample A and sample B for three (3) days, it was then heated up until
the ethanol evaporates. Afterwards, it was then weigh using analytical balance. The two
samples were then again soaked with rubbing alcohol up until it acquires residue from the
sample. The samples were removed and the flasks containing the residue were putted into the
oven. After it dried, the flasks were weigh again.

d. Calculation

In order to calculate the % crude fat, this equation is used:

weight of fat flask ∧residue−weight of empty fat flask

% crude fat= x 100
weight of the sample