S40 Abstracts / Pancreatology 18 (2018) S1eS188
1
function test, vitamin D levels and some imaging technique of the
pancreas. Treatment algorithms have been published by different expert
boards. In patients with preserved C-peptide and pancreatic exocrine
insufficiency (PEI), enzyme supplementation should be started first as it
might improve symptoms, prevent malnutrition and normalize incretin
function. Metformin might be added if necessary. If there is no C-peptide
capacity, insulin treatment should be started following the same recom-
mendations as in other diabetes types. Glucagon insufficiency has to be
considered, leading to a higher rate of severe hypoglycemia in some
studies.
Conclusion Pancreatogenic diabetes bears some peculiarities con-
cerning diagnosis and treatment that have not been realized by a majority
of diabetologists so far.
P2-079.
Preparation And Evaluation Of Eudragit Coated Chitosan Microparti-
cles For Oral Insulin Delivery
Aakanchha Jain 1, S.A.U.R.A.B.H. Bhargav 2, S.O.U.R.A.B.H. JAIN 1
1 an increased prevalence in those with CP (65.2%) and solid PT (65.7%) and
Bhagyoday Tirth Pharmacy College, Bhagyoday Tirth Pharmacy
College, Sagar, Madhya Pradesh, India
2 pancreatic cancer (PC) had abnormal glycemia, and almost one in three
United college of pharmacy, Pharmacy, Lucknow, India
Objectives Diabetes mellitus was known in relic and vestiges at pre-
sent a global and ascalating health problem with millions of cases being
diagnosed daily. Diabetes can affect numerous parts of the body and is
associated with solemn complications, such as heart disease and stroke,
blindness, kidney failure, and lower-limb amputation. Some complica-
tions, particularly micro vascular (e.g., eye, kidney, and nerve) disease, can
be reduced with good glucose control.
Methods The microspheres were prepared by membrane emulsifica-
tion technique. The prepared microspheres were observed and charac-
terized for physical properties like particle size distribution, surface
morphology, zeta potential. Subsequently, the microspheres were
employed to load model peptide of insulin. The in vitro release profile for
the optimised insulin-loaded microspheres was investigated.
Results Under the pH conditions of gastrointestinal environment, only
28% of insulin released in the gastro-intestinal simulated fluid covering the
transit time of drug (2 h in the stomach and 4 h in the intestinal). While
under the pH condition of blood environment, insulin release was stable
and sustained for a long time (14 days). Furthermore, the chemical stability
of insulin released from the microspheres was analyzed after treating with
the simulated gastric fluid containing pepsin for 2 h. Finally, the blood
glucose level of diabetic rats could be effectively reduced and stably kept
for a long time (60 h) after oral administration of the insulin-loaded
alginateechitosan microspheres.
Conclusion The prepared alginate coated chitosan microparticles, with
mean diameter of about 1.5 mm, was suitable for oral insulin (peptide)
delivery. Moreover, alginate coating onto the surface of chitosan micro-
particles could modulate the release behavior of insulin from alginate
coated chitosan microparticles and could effectively protect its degrada-
tion in acidic medium in vitro for at least 2 h. In all, the prepared alginate
coated chitosan microparticles might be an effective vehicle for oral
administration of peptides.
P2-080.
Pancreatogenic diabetes mellitus e Prevalence and risk factors
Daniel Vasile Balaban 1,2, Andreea Zoican 2, Marina Ciochina 2, Georgiana
Robu 2, Ionel Petrut Nuta 2, Raluca Simona Costache 1, 2, Florentina Ionita-
Radu 2, Mariana Jinga 1, 2
'Carol Davila' University of Medicine and Pharmacy, Bucharest,
Romania
2
"Dr Carol Davila" Central Military Emergency University Hospital,
Gastroenterology, Bucharest, Romania
Objectives Pancreatogenic or pancreoprivic (type 3c) diabetes mellitus
(DM) represents the diabetes that occurs secondary to diseases of the
exocrine pancreas e chronic pancreatitis (CP), post-necrotizing acute
pancreatitis (NAP), pancreatic resections (PR), and pancreatic tumors (PT).
Although it’s a relative common finding in gastroenterology wards, it’s
often underdiagnosed or mislabeled as type 2 DM, although their clinical
and laboratory characteristics are different. Our aim was to evaluate the
prevalence and risk factors associated with pancreatogenic DM.
Methods For this observational study we included patients with
pancreatic pathology (CP, cystic/solid PT, PR, post-NAP) who were
admitted in our gastroenterology department in the last 24 months. De-
mographic, clinical, laboratory and imaging data were collected. We also
noted their status as diabetic/non-diabetic, and for the first group we split
them in preexistent and new-onset DM.
Results Altogether 102 patients were recruited, mean age 54 ± 14
years, 61% male, of whom 46 had CP, 45 PT (35 solid tumors, 12 cystic
tumors), 6 PR and 5 post-NAP. DM was present in 61.8% of all patients, with
the lowest in those with cystic PC (33.3%). Over two thirds of patients with
(32%) of them had new-onset DM. Age over 60, weight loss and new-onset
DM were associated with a higher risk of PC.
Conclusion DM is a frequent association in patients with pancreatic
disease. A new-onset DM in an elderly with weight loss should prompt
screening for PC.
P2-081.
Evaluation of antidiabetic and hypolipidemic activity of cedrus deo-
dara and embelia ribes with their synergistic effects
Sourabh Jain 1, Aakanchha Jain 1, Saurabh Bhargav 2
1
Bhagyoday Tirth Pharmacy College, Sagar, Madhya Pradesh, India
2
United College of Pharmacy, Pharmacy, Lucknow, India
Objectives Herbal medicines are getting more importance in the
treatment of diabetes, cancer and hepatic disorders because of the side
effects of the current allopathic therapy. The medicinal plants Cedrus
deodara and Embelia ribes are rich with antidiabetic active contstituents
like flavonoids, tannins and embelin. Former studies showed that both
pants are potential for the treatment of diabetes. The present study was
undertaken to study combined synergistic effect of hydro-alcoholic extract
of heart wood of Cedrus deodara with Seeds of Embelia ribes on blood
glucose, insulin, and cholesterol and triglyceride level in normal and hy-
perglycemic rats.
Methods The antidiabetic and antihyperlipidemic activity of hydro-
alcoholic extracts of Cedrus deodara with Embelia ribes (50: 50) was
evaluated by using normal and alloxan-induced-diabetic rats. The acute
effect of extract was evaluated by administering 400 mg/kg to normogly-
cemic rats. In the chronic model, the aqueous extract was administered to
normal and alloxan-induced-diabetic rats at dose of 250 and 500 mg/kg
per day for 21 days. Blood glucose levels were monitored at specific in-
tervals and different biochemical (LDL, VLDL, HDL, Cholesterols) parame-
ters were also carried out. Histology of pancreas, liver and kidney was also
studied.
Results The statistical data indicated the significant (P< 0.05) increase
in the serum insulin level and decrease (P< 0.01) in the blood glucose, total
cholesterol and serum triglycerides. HDL cholesterol level was significantly
(P< 0.05) increased when treated with the extract. Histology of diabetic
rats treated combined extract with showed the pancreatic b-cells
regeneration.