Unit 3 - Plant Tissue Culture

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Pharmacognosy & Phytochemistry I
Practice Question Paper (Unit-03)
Plant Tissue Culture
B.Pharmacy- 4th Semester (New PCI Syllabus)
Note- All these questions are made by “Solution-Pharmacy” which are based on
our video lecture. Our objective for making these practice questions is to prepare
students for their examination. Total marks is- 75
Section 01- Multiple choice questions. (Attempt any 20 questions) 01marks each
1. The cultivation of plant tissue or organ of the plant in artificial nutrient medium is
called as
A. Genetic engineering
B. Plant tissue culture
C. Hybridization
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D. Biotransformation Contact- solutionpharmacy@gmail.com
2. Ability of single cell to divide and develop into whole plant

A. Plasticity
B. Totipontency
C. Polyploidy
D. Multipotenty
3. In callus culture, higher concentration of Auxin as compared to Cytokinin induce

the formation
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A. Adventitious Shoot Available on- YouTube-Facebook-Instagram
B. Adventitious Root
C. Bothe Root and Shoot
D. None of the above
4. Haploid plant develop by

A. Anther culture
B. Root culture
C. Leaf culture
D. Meristem culture
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5. Which of the following is not the component of Nutrient medium for Plant tissue
culture
A. Plant growth regulator
B. Sucrose
C. Globulin
D. Inorganic salt
6. Plant cell without cell wall is known as

A. Chloroplast
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B. Protoplast Contact- solutionpharmacy@gmail.com
C. Chromoplast
D. Leucoplast
7. Plant part which is used for tissue culture technique is called

A. Plantlet
B. Embryo
C. Explant
D. Scion
8. Father of Plant tissue culture technique

A. Braun
B. Hanning
C. Glautherate
D. Gotlib Haberlandt
9. Somatic hybrid is developed by

1. Seed Culture
2. Protoplast fusion Solution-Pharmacy
3. Layering Available on- YouTube-Facebook-Instagram
4. Grafting
10. Type of culture required for secondary metabolite production

A. Seed culture
B. Leaf culture
C. Suspension culture
D. Bud Culture
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11. Synthetic seed obtained by encapsulating
A. Somatic Embryo
B. Somatic seed
C. Somatic cell
D. Protoplast
12. Embryo culture is carried out in 1904 by

A. Braun
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B. Hanning Contact- solutionpharmacy@gmail.com
C. Glautherate You can download all of our uploaded study materials by joing our "Solution-Pharamcy" facebook group and tehn go to file section
D. Gotlib haberlandt
13. Which bacteria is used for the hairy root culture (genetic engineering in plant)
A. Agrobacterium rhizogenes
B. Pseudomonas aeruginosa
C. Yersinia pestis
D. Chlamydia trachomatis
14. First transgenic plant

A. Potato
B. Tobacco Solution-Pharmacy
C. Datura Available on- YouTube-Facebook-Instagram
D. Tomato
15. Enzyme required to produce protoplast

A. Cellulase and pectinase
B. Pectinase and Zymase
C. Protease
D. Pepsin
16. Process of transferring callus in fresh medium for the maintenance of its growth
A. Inoculation
B. Subcultering
C. Incubation
D. Biotransformation
17. Commonly used solidifying medium for tissue culture medium

A. Gelatin
B. Starch
C. Agar
D. Pectin
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18. Most commonly used carbon source in plant tissue culture medium
A. Maltose
B. Starch
C. Sucrose
D. Cellulose
19. Optimum pH of prepared nutrient medium before sterilization

A. 4-5
B. 5-6
C. 6-7
D. 7-8 Solution-Pharmacy
20. Protoplast consist of following except

A. Cell wall
B. Cell membrane
C. Cell wall and Cell membrane
D. Mitochondria
Section 2- Long answer type questions (Answer any 02)

1. Write the different components of nutrient media and explain in detail about role of each
component in tissue culture technique.
2. What is Plant tissue culture? Explain the different types of plant tissue culture with its
use.
3. Explain in detail about different application of Plant tissue culture.
Section 3- Short answer type questions (Any 07) 05 marks each

1. Write short note on growth and maintenance of callus culture
2. Write brief note on growth and maintenance of Suspension culture
3. Write down the history of development of plant tissue culture.
4. Define Edible vaccine. Write its advantage and Application.
5. Write short note on protoplast culture.
6. What are the basic requirements of plant tissue culture?
7. Write the general steps involved in plant tissue culture.
8. Explain the growth pattern of suspension culture.
9. Briefly explain hairy root culture.
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2. Ability of single cell to divide and develop into whole plant

3. In callus culture, higher concentration of Auxin as compared to Cytokinin induce

4. Haploid plant develop by

6. Plant cell without cell wall is known as

7. Plant part which is used for tissue culture technique is called

8. Father of Plant tissue culture technique

9. Somatic hybrid is developed by

10. Type of culture required for secondary metabolite production

12. Embryo culture is carried out in 1904 by

14. First transgenic plant

15. Enzyme required to produce protoplast

17. Commonly used solidifying medium for tissue culture medium

19. Optimum pH of prepared nutrient medium before sterilization

20. Protoplast consist of following except

Section 2- Long answer type questions (Answer any 02)

Section 3- Short answer type questions (Any 07) 05 marks each

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