® BTIOle Bio process P Monoj End Sem MEISBIER O A stain of bacteria is vent efbiciemt c coribon dioxide ot conventtin at mosphor! to gucose: TH Ue Unio Shoal? yal scale we need bio seoctor pioweacto* on indust oO Design of Bio Neactox 1S O vessel for He mickooxganisms bio weactos iS to psovide & suitable emvisonment in Which on oxeanism can efficiently produce A tasiget peeduct » Celk biomass 2 Metabolite e Bioconvension product growth of Functon Of penfosmance depends ont 1) Agi talon sate 2) oxygen franskn 3) pH 4) Tempenoture s) Foam psoducHon Design facto¥ 5+ oprmal operateg aa psoduct value ole of produce Big sgactos consicenatens eg ») ripe eae Should be gent ly asyated ond 2 - conn ) wetl Lonpalled homogemesus evixenmenral anda supply of CO) - nvr! OLN dJiHON3) A lange support mateniad Susfoue _ volun sali needs +o be provided fox ANChorage- clepenclent cells. 4) The xemoval of toxic products of metabolism, Such ag lacHc acicl ond ammonium, and the ConcemPiaHor) of high- value produc, monoclone! anthodics, lymphokines Should be established - A typical bio seactow cansiskh Of ¢ 0) Agi tatov 2) Batlle 3) sponge? 4) Jacket: Types of BioweactoxS In Todvstry - +) Shisxed tank eat toy 2) Bubble columm seactor 3) Aix-liFE loop seactox 4) Psopellew loop seactoy 5) Tek loop seactoy High pwoduckvity , yield and high product concemPraHon one mojor objectives of plant bizeve proce backenia seraim industria! use: There pes of bloxeactor provide geriowHhy vonious factOss Vike vessek Shape y “agitation ; aenation, bafHles etc play major ole “7h psoduclivity qhese ig no bioweactor that can satisty au Oooo There ave othen novel opproathes iNCSeAaSe pso cli ly of Spangens, agitators as wel as vanious conbrol li olling Byob 4o. IMPoOVe PsoducHvity op br 23 IN oxden ,® ’ P- manoj Co Ulbsailtsa Hon MEIZBIEQ Tt is o type of membrane Filbsation. Hené forced HIKE puesste O% concemts Hon geadient ead +0 o Separation, Hare sepaxahor happens theong h a semipermeable membrane Ul tsa fileiaHion ee gimilon fo micvoH|PiaHon These membranes have. anisotsopic Suche: TH applications 94 eFicient and eonPeit ea dy) used fe concentsate chemicals and Ibiofog cots ot a high degree of posi dia til sakicr. AD ConcenPtaton , punificalon and : Concem baton ! wat is semoved Croce a queous Solv HON of soluk purification ~ Solvent amd Jous molecuta weight producls ane sepanaed from high molecvion weight Products: dio cilPsalion ‘- Jou moleculon weight solutes Such ab Sal , SUgars and alcohols Poss Hhoough the fillers and rhe setained Stseam contains the peoduct The penmeotte that Leaves the System 1S seplaced with deionized woattn. (ii) Ufksacentsifagation This i @ technique in which components of Q soavulon one separated based On / then size and density: Densen the biological spruce is the fasten it Sedi i a Cembrifugal Feld: ae‘The qm . = greater the cenbriFugal foxce iS, the fasten the particle Sediment ApplicaHions of Analytical and Preponative UlPracemtbri Fuga Hon: a) detenminahon of putiry and ave Moleculan moss of Soluotes dy Skudy of chong &3 in molecuian Mar of supramolecular complex 23 Ud pelecHion of confosmation and contexmational chong es a subcellulan fyachonaton VV) Visus punificaHon Wy sepanaHhon of DNA componen'® oy Colloid sepa7ahon Jil) Reverse osmosis :- Tt (S a waten puni fication process that uses a posttiolly permeable membsane to seposiate ions: Unwanted pon tcles fom inking watn con be siemoved by Mais on) : process? Here applied presscore 1s used +0 Ovencome osmotic pressure Applications '- ly Psinking wotn punificaHon Tl) Maple sysup psoducton- dy Fos smoall-scole Inydzogen- prodecton MeVENSE Osmosis is somehimes used : to prevent fowmation of minenal _cleposit& on he Suntace of electrodes6) 1S anothen & pplicakor Jy Window cleaning of wevense osmosis Used for woshing +he windows pmMono) ] meg R162 Ny Gravity Sedimentation , io the step in Hof follows ion and precedes cilbroton: 16 jo #o enhance He fileoHon | proce’s by siemovin Tt 6 physical woakn #1 Using gros +o wemove SUS fom waten-. (qvev' hy Se dimentoaHon lneatment syslems wated £locculat prsrpo se a po Yicvlal® mo eatment proces lids pended So Applica Hons Potable water reatment i Generally follows a siep of chemical d LlocculaHon , which allows flocs of coagulalon an gsouping ponticles togethen into a biggen size: 2) Pximan lseatment of sewage EE Floaling and settleable solids Ersrough cedimemtat¥enol Noses el mi moti 5 osmotic LIT osmotic uy Revense © en oe quills ex = cf [ alle | sunt o & a FAN cating Wate, “Wate Semi permeable membyane ict, [pralon eye In Pp. MANOJ MEIRBI62 . and Sexeeni Sane oO AL tao A J : a : ING mic of RAY i YW o \ yale) : | ota. ao f o, 20 - o£ . > ° 20,2 > Ulte Ailtened \ / He 2 °O hen ¥ : ] o% ve | ware | | pigsolve d raumy Bacteria sal vixus oxganic Maeve molec !€3 ization of cells and egymes Te SS Cel immobilizakon ig one of the eX Citing of bio proces) Tmmobilized celh - to feamemtahon of «weactow penfowsmance immobilized cells provide olen). te QUCEE weducing | advam tages thot freely, suspemded oven fox moxwe efi ciemt openation by the non- pxoduchve geo Hh plaase - High cell demsiky of immobilized cells one product yield ond volumetric Pso Sore of bio weachows: 4P Manoj @ protectS the cellS MEIZBIG2 Tmmobilizaton import from shear foxcez and oO cpecial stability Lo the microoxgonis™ agains gE 33e3 J gnmobilizect cello con emv ison mentoll eosiluand fon be handled more the soluHon coi tnout di Flicul KY" ~ecove sed co free peoduct slneam simpli bes dow nshicom puocPAing bio proce» openahon componien Free cell Fn obi li ze¢ cold ‘| Batch ,fed batch: o¥ JBatch os fed batch proces fenmemtat on using sHwsed tank seactoy conHnous 0 pre Hon moa fenmento¥ ay lenge Leamen tr vy s Sequised ssequixed . it ; c ffechive Sepanaton big Simplited proc es Bees One eC] design WW) cells cannot be ‘iv » Reuse ot cells fox proton ed peniod ot Vv ; Hm WY) product quality vy Long eam tobi li YY ond constant product quality Tinmnobilized emgyme ~ Tr is am enzyme that is attached to Oo matenial that 'S jnent and insoluble - Tt otlows the emg yes to be Stationary Hetough od the process 5 cohich makes It = : och easien fox them TO be ceparaled ond ‘xeusedBro procera Adsospion ” simplest and quickest methods Of weversible enzyme immobr |i zo HON method is inexpensive ound = promoles easy conract bekuee) emayme cund supproxt Entrapmemt ° Tovowes aging Fhe emgymes | VaniCIS materials: Tt allows inceeased mechamical Stability ond easy contact between SUE amd enzyme Cc ~oss Hiaking _— Ixvevensilble method oF enzyme im mol |i zalier Known aS cannien fee immobilizatton emzyme acts asik ewn cannien Encapsulation *~ Method tavolves emclosing Enzyme Ina membyane capsylé made of Semi permeahie matervocy effeclivenerh is based OM Stolo/! of enzyme being used + Tnexpemsive allows fox lange amounls of em zymes to be immobilized - Lew Covalemt bonding aa issevensible proces tnvolves Coverlet ly bindiry proteus +O INsoluble suppowt Meateucy ~ _methad makes j+ POSsible to be weused iN moxe for MwmMzymes Cacles Hham then meted6 ecovery ese labor, Space ig weuse amd Abi li Hy fo w ° ma jos use cost effecHveners* : eee macnes cu prod cHon > ° Tmmobilized enzymes have wide of potentcot opplica Ean? emzyme play a KB abili lH es consistent Supply yoni Hy . Tmmobilized rajor sole vaniousS manke to keep in products in centro 4) Primo cell bio-proceming iD vaccine pwoducHon ‘.- - Sevenah vaccines fox amimel om VyisUs have beem psoduced d humam vse In Some iS propagated in ig then collected animal cells » VIsVs end = inackvated amd used OS @ vaccine Tn other = coeak ened ox atkenvuated kexm of the yisus tS Used at 1) induce & psotech ve sesponse but MO dicease. However, most vaccines mouwd Unden development ane SUbUaIT Vaccines Tupicolly psoten displayed 7 the vixus porticle ig psodeced: gituaHons live cuntoce of fhe An epitope OX smoll segion of the pxokin 7 secognized by the immune Syskem and duces & psotecHve immune ~sesponseFox the epitope +o be displayed propenly, p.mene, ® the psotewn moy need +o assemble Fi MEIZBIGD (nto a visuslike pottcle Such @ poxthcle 1S aon emply capsid that contauns no Nuclerc acid The mbsence of vite PNA O% eA incs eases sareky of fhe psoduct: cellS us i ed fow voccin€ produchon . Psimoxg Cells: pores cet coltunes CON be established om : sate amimals ox @nbryos ox Prom selected tissues fsom embsyos, New bosn animals ox adultanimals: e Diploid celh lines Acceptomce of Hhese cell Vines is based on chonactnizaHon cata Showing & nosmal chromosomal pee Potoat ° Conttnous cell lines Mave been used fox psoducton OF Sate omd ellecHve bio- tHhenapeyHcs Vaccines one moinly psophylache and Somelme phenapeoHl | they sepsesemt bio- phanma cevtteak psodu ck makina thew development and camencialization Compler - Acceptance of cell substrates Can be achieved by Hein evaluaHon and ChanacterizaHon on a Case by case basi sepneael © EIS BIED 5) DT tink Hene ave many ospects fo be considened be fose coming to conclusion uohe then oe I™my shovid be aiven Fable Or dnugs which shonpems Hein physiced and mental abilities. Militouie USsia Vooui0US perfor mance ability +° sustain oot wide hove used op ane psychoar hve dnugs +° improve of solctiets b suppress in hunge, uithout food , incseasin endwrance of high altitudes and spoxpens heen physical and mento abilite4 examples of these daugs used powa concents® Hom, Same -dloys in euro Ex ce Moda Finis Soldiens have Faken Hhese fobiers to help them Fight: these ane also used by non- state oamed soups +o xeword cod combot penfoxrmonce, ond to ovencome Ppoex Living Conditions ; Hey one used tO secsuit mew fightens: Use of dug s +o emhance penforrane prey be chealng in Spoxts pot wo is not | Oo SposHn event Uniquely ia mili footy openations , technology ond training aim to achieve a CompeHHve adventage, not a fain chance of succers: The notuse of anfane involves life and cleath consequencesP.Mono bility and Hhe MEP BIE? solders The physical Suowive moxol esponsibility hos implicahons not onle bus onre we lied demanded OF ia combat fas beyond the individual, Sold iens nepresent ) society ond its valves > on 6 protec ond psesesve Hhem y Fhe moxas and ethical thoesholds in cet ane diffenemt trom those in peace Lime one Not society »but mhey made in conflict independent from it - Decisions serlect the yalues OF the socieky ON woh behalf soldiens Fobt, cand in inbrodecig new technologies 5 such &3 the phoormea coreg 1 @ enhance ment of Soldies, these ethical values must be protected ~ Tn oddiHon any new intsoduced to Khe military must 5 Gohich technology considen the Deood Promewosk unden soldiens operate , international hymanitaniam Low 5 includin humam sights Daw, Ac his technology and selevont cdomesHc Laws: ts still emengent 5 the outcome OF this be to deine & Legally and emgquixy will erhically Just fiable Framework of the seseosich and development of pharmacological technolagies in militany seHbung °