GATE-1999 tof le | LIFESCIENCES sss H: CHEMISTRY (COMPULSORY) 1314 J mor" = 0.0821 1. atm, mor Kt Faraday constant = 96500 eoulombs Gas constant , For each sub-question given below, four alternatives are provided of which only one correct. Write the correct answer in the answer book by writing a, b, ¢ or d along with the corresponding sub-question number. 20x 20) 1 For the two gases and y the respective molecular weights are 40 and 28, The corresponding maximum heat capacity Values (Cymax) are 12.43 and 29.09 JK! mol! The heat capacity ratio (cy/¢,) are found 10 be 1.666 and 1.286 respectively. ‘The atomicity of the two gases will be a x=Ly=3 box=Ly © x=2y= do x=Ry One mole of an ideal gas (cy = 20 JK! mol ') initially at STP is heated at constant volume fo twice its initial temperature. For this process w and q will be a w=0.g=S46KI bow ey 546 KI do w=-S46KI, q=546 KI 3, The eryoscopic constant for Water is 1.86. A 001 molal solution of aqueous acetic acid produced a depression of 0.0193 °C in the freeing point, Then the degice of dissociation of acetic acid is a 0% b. 7.2% © 36% d 36% The solubility of the given electrolyte is 1.3 10% moll! and Ke is 1.710" The electrolyte is made up of a. uni -uni valent ions ©. uni-in valent ions 4. di-tri valent ions The equilibrium constant for the solution phase equilibrium of KI+ I> Kix is Ky at T.. The value of the equilibrium constant K: for the formation of Kly at higher temperature T> (T> =27)) will be a K)=K: b Ke da. [HgCh A compound of silicon with a high hardness yalue (9.15 Mohs scale) is a. Cas b. SIC c. Na,SiOy 4. Mg;Si Benzene is first subjected to mono nitration followed by bromination. ‘The product formed is 4 ortho-bromo nitrobenzene b. para-bromo nitrobenzene ©. meta-bromo nitrobenzene 4. sym. Tricbromo benzene ‘Treatment of phenol with chloroform and aqueous alkali leads to the formation of a. Resorcinol b. Benzaldchyde & Benzoiec acid 4. Salicylaldehyde ‘The order of reactivity of alcohols towards dehydration reaction is a, 22>)" b, B>2>3? © 25193 d >>?” 1.3-butadiene when treated with p> benzoquinone in benzene at low temperature (35°C) leads to the formation of a. Hydroguinone b. Benzaldchyde ©. Naphthalene 4d. Tetrahydro 1, 4-naphthequinone Among the following alkynes which will form insoluble acetylide with AG”? 4. 2-butyne b. 3-pentyne © Lebutyne 4d, S-hexyne Match cach item in column 1 with the ‘most appropriate one in Column 2(5x1= 5) Column 1 . Energy of activation . Mereurous chloride . Graphite . Cryolite . Geometric isomerism Column 2 2. 1,2«dibromo ethens 3. Walden inversionHaber's process Arrhenius equation calomel Raoult’s law Fused salt electrolysis Answer the Following A. The normal boiling point of benzene and ethanol are found to be 798°C and 78.2'C respectively. Ifthe corresponding values for the enthalpy of vaporization of the two liquids are 30.76 and 38.57 kJ mot calculate the entropy change for the vaporization process of the two liquids. Comment fon the molecular structure of the liquids. @) At 25°C, the ionic product of water (Ky) is 5.35 10", What will be pH at this temperature ? Q) Answer the following A. The standard free energy of formation of NOx(g) and N:O,(g) at 27°C 51.31 and 9789 KI mol! respectively. Calculate the K. for the reaction 27°C, N,O,x(g) | 2NOx(g) Chloroform and carbon tetrachloride form an ideal solution at 300K this temperature the vapor pressure of chloroform is 200 mmHg and the of carbon tetrachloride is 116 mmHg. Determine the vapor pressure of solution containing equimolar mixture of the two components Answer the following A. The standard reduction potentials for the reactions are given as follows Sn" 42e-+ Sn E°=-0.136V Sn’ 4 2e—» $n" E°=0.150V Calculate the AG® for the reaction Sn Fe Sn ‘The molar conductance at infinite dilution of KCI, KNOs and AgNO: pressed as mho.mol”'n, are 0.01499, 0.01450 and 0.01334 respectively What is the molar conductance of AgCl at infinite dilution? Answer the following ‘A, The following nickel complexes, [NiCL] and [Ni(CN),)° are found to have different magnetic properties. Zot Ii Wentify the magnetic properties. and explain on the basis of Crystal field theory. [Ni = [Ar] 3d° 45°] To an aqueous solution of cobalt complex having the formula CoCI.SNHs silver nitrate solution was. added. How many moles of AgCl will be precipitated per mole of the complex? Write down the structure of the complex. Write the structure of the compounds A. B, C.D and E formed in the following sequence of reactions A Nitrobenzene 2 MCh, step B, Ia see nse a HO 1: BIOCHEMISTRY For each sub-question given below. four alternatives are provided of which only one is, correct. Write the correct answer in the answer hook by writing a. b, ¢ or d along with the corresponding sub-question number. (29x1=20) Which of the following statemins is incorrect ? a Myoglobin consists of a single polypeptide chain m addition to a heme prosthetic group ‘The red colour associated with the oxygenated forms of myoglobin and hemoglobin is due to heme. Hemoglobin has a higher affinity for oxygen compared 10 myoglobin as seen from the oxy gen binding curves ‘The «and fi chains of the hemoglobin tetramer are slightly shorter than the single chain of myoglobin The molecular structure of a disaccharide is given below. Identify the compound ort noetls okt a. Lactose b. Maltose c. Sucrose4. Cellobiose J chain is a glycopeptide chain associated with which of the following immunoglobulins? a IgA b. IgG © IgM 4 isk Which statement about ribonuclease is correct ? 4 Ribonuclease is an evonucleases b, ‘The enzyme mediated cleavage occurs at pyrimidine and purise nucleotide residues ‘The hydrolysis product isa polynucleotide with a 3*-phosphate terminus, L The hydrolysis product isa polynucleotide with a 3-phosphute terminus, Molecular weight of an unknown protein ean be found out by a, Electrophoresis b, Ion-Exchange chromatography ©. Affinity Chromatography 4. None of the above techniques N-terminal amino avids are usually determined by Sanger’s method using a. Ninhydrin Reagent b. 2,4, dinitrobenzene ©. Hydrazine 4d Concentrated Nitric Acid Cleavage of the following peptide with Chymotrypsin Hy N— Gly Arg—Ala—Ser— Phe —Gly ~ Asi -Lys-Trp- Glu ~ Val COO. results in a, 2 fragments b, 3 fragments ©. 4 fragments d. No cleavage ‘The average molecular weight of an amino acid in a protein is a 112 b. 109 e 110 4.137 40h 16 Which of the following peptides can be easily detected by absorbance at 280 nm 1, Leu — Tyr _Met— Ala — Glu Ser ~Thr—Thp~ Val —Tle Leu . Ac ~ Ala ~ Glu ~ Gln ~ Ser ~ Asp Lys Thr —‘Tyr Trp — Val — Ie 1,2, and 4 Vand 4 2and3 2and4 Phospholipids containing cyclopropane rings have a. A higher Tm than saturated avy! chains of the same length b. Closer packing of the acy! chains in the gel phase ©. Greater fluidity in membranes that they form a part of 4. Allof the above ‘The oxygen saturation curves for two oxygen binding proteins are shown in the figure below, Fach protsin binds four oxygen. Which protein has a higher affinity for oxygen ? | | ee - rel? Zw / Ba Bi - Fs pear te Protein A . Protein B Both Proteins A and None of the proteins The formation of glucose in photochemical reactions involves the reduction of CO: with the production of Os. For this process which of the following agents is responsible a. FADH b. NADPH! c, ADP d. None of these Although T-cell and B-cell membranes contain some shared reception, the receptor specific for B-cell membrane isa. Pokeweed nitrogen (PWM) Phyto hemaggintigin (PHA) Concanavalin A(con A) Sheep red blood cells (SRBCs) Most antibodies are synthesised by the 4, Central lymphoid organs b. Peripheral lymphoid organs ©. Primary lymphoid organs d. Macrophages Of the immunological commonly used 4. Enzyme-linked immunosorbent assay (ELISA) can be used to assay antibodies only Immunoelectrophoresis can be used to determine the concentration of IyG because ofits cathodic migration ‘The precipitation pattern in immuno- diffusion cannot detect antigenic relationship |. Radioimmunoassay (RIA) is used for quantitation of any substance that is immunogenic or haptenic Limmunosuppressive measure are most effective when administered a. Just prior to antigen exposure . One week before antigen expostre © Atthe lime of antigen exposure 4. Following antigen exposure Which statement about phosphorylation is not true The five assemblies of proteins and cofactors involved include the electron transferring complex I-IV and ATP synthase. ‘The mobile carrier ubiquinone tinks complexes I and I with complex 11 and cytochrome C links complex Ill with complex IV, For every two electrons iransferred from NADH to Q by complex 1, two protons are translocated 10 the intermembrane space. FAD, FMN, iron-sulphur chusters and copper atoms are other cofactors that participate in electron transfer. Z DNA is a third type of DNA helix. with structure a. CGATGC b. ATGCCG techniques oxidative 2 ¢ GCTAAT 4, cGcacG The TATA Box plays a key role in assembling active transcription complexes by & Binding with protein b. Binding with DNA protein ¢. Binding with RNA protein 4. Itdoes not bind with any protein ‘The order of the predicted rate of diffusion across a lipid bilayer (highest to Lowest) is a. Toluene, galactose, phenylalanine, chloride ion b. Chloride ion, phenylalanine galactose, toluene © Galactose phenylalanine, chloride ion, toluene 4, Toluene, phenylalanine, chloride ion Match the entries in column 1 with those in column 2 and write the matching pairs in the answer book. (Sx1=5) Column 1 A. Hexokinase B, Pyruvate dehydrogenase CC. Aconitase D, Succinate dehydrogenase E. Ribulose diphosphate carboxylase Column 2 1. Isocitrate 2. phosphoglycerate 3. Glucose 6-phosphate AL Acetyl CoA 5, Fumarate Answer the following A. What are the immediate consequences of limitation in the following, factors ‘om the rate of photosynthesis ? Light and CO, ‘The rate electron transport The activity of RuBisCO @) . "The two ionizable -OH groups of the phosphate group in a-D-ribose 5- phosphate have pKa values of 1.2 and 6.6, What is the structure of the predominant form at physiological pH? Answer the following, TATA Box-binding galactose,In aqueous solutions below pH 3.0, the synthetic polypeptide polyglutamate, forms regions of a-helices. Above pH 5.0 it assumes an extended conformation. Explain this observation, Q) At what pH would polylysine be likely to form a-helices ? a) In glycolysis, there are two reactions that require one ATP each and two reactions that require one ATP each and two reactions that produce one ATP each. This being the case, how can glycolysis of glucose to lactate Iead to the net production of two ATP molecules per glucose molecule? (2) Answer the following = A The transtormation of glucose to fructose-6-phosphate occurs. as follows glucose ~ ATP —> glucose-6-phosphate » ADP AG?=—4.0 keal’mol Glucose -S-phoshate > fructose-6- phosphate AG? = =40 keal/mol Caloulate the standard free energy change and the equilibrium constant for this transformation at 25°C. (3) B, What are the main features of a zine finger’? 2 Answer the following. Ac Th vitro sequencing reactions are commonly carried out at 65°C and usually employ a DNA. polymerase isolated from bacterial that grow at high temperatures. What ts the advantage of this ? @ What are the main features of the enzyme linked immunosorbent assay (ELISA) method ? 3 Answer the following : A. The chromosome of a certain bacterium is a circular, double- stranded DNA molecule of 3.2 s 10° base pairs, ‘The rate of replication-fork movement is 1000 nucleotides per second, Calculate the time required to replicate the chromosome, (e) Suppose you want to express the fi globin gene using an expression Got 16 vector. What factors are important for maximizing the efficiency of Iranseription and translation if’ the expression Vector is 1, acukaryotic vector 2. a prokaryotic vector 3. a fusion-protein vector 4; SOTANY For each sub-question given below, four alternatives are provided of which one is correct. Write the correct answer in the answer book by writing A, B, C or D along with the corresponding sub-question number, Q5x1=25) Which of the following compounds is the immediate source of oxygen produced by photosynthesis? a. Carbon dioxide b. Carbon monoxide &. Water 4. Chlorophytt Cell wall polysaccharides are synthesized mainly in a. Cytosol b. Golgi apparatus ©. Endoplasmic reticulum d, Plasma membrane Which of the following is the most abundant protein in leaves? a, Chlorophyll a/b binding protein b. ATP syntheses & Rillose-1S.bisphosphate carboxylase 4. Globulins What roles do opines play in crown gall diseases? 4. Source of carbon, nitrogen and energy for the Agrobacterium b. Transfer of TDNA to plant cells. c. Attachment of Agrobacterium to the plants 4. Induction of the expression of vir genes Lettuce (Lactuca sativa) belongs to the family a. Craviferae b. Composite© Cucurbitaceae 4. Rosaceae Mitosis occurs between a. Gland $ phase b. S phase and G2 phase ©. G2and G1 phase dS phase and G1 phase Ih plant tissue culture studies one of the major problems is the production of polyphenols in the media. This can be tackled fo varying degrees by the inclusion in the media of : a. Agar be Vitamins Sucrose 4. polyvinyl pyrrolidine The enzyme nitrogenase catalyzes the reduction of dinitrogen to ammonia. This molybdenon containing protein requires the following of the reaction a. NADPH, Fe b. NADPH, Heme c. Reduced Ferredoxin, ATP, Fe 4. NADPH, ATP, Heme Tn which of the following regions of the electromagnetic spectrum will Chlosphyll absorb light maximally a, Green b. Red &. Yellow d, Far Red Which of the following phytohormones play a role in seed germination 4 Gibberellins b. Cytokinins ©. Ausins d. Abscissie acid Tonoplast is defined as a. the plastid of meristematic cells b, the membrane of plant vacuoles s, the membrane of mitochondria 4. the membrane surrounding the chloroplast Which of the following contribute to evolutionary changes ? 8. Comparative anstomy bb. Fossil formation ©. New genetic combinations Tol" 16 4. Lack of gene pools ‘The genome of cauliflower mosaic virus is a. Posifive-stranded RNA bb. Single-stranded DNA © Double-stranded DNA 4. Double-stranded RNA Which of the following statements about plasmodesmeta is correct ? 4. Like gap junctions, directly connect the eytoplasm of adjacent cells b. A. new site of microtubule and microfilament assembly that appears during late telophase ‘The state of four to six flattened sacs of Golgi apparatus ‘An interconnected network of fibrous proteins that is important in cell structure and for intracellular transport In a double standard DNA. the possible number of reading frames is a2 b, a4 a6 Parthenium is a. aK selected species b. anrselected species €. None of the above 4. Both of the above What percentage of the incident solar energy do plants typically harvest during photosynthesis? a 1 b. S-10% 10-20% a. 20-50% Photorespiratory pathway in plants involves close cooperation of chloroplasts, mitochondria and a. Glyoxysomes b. Peroxisomes & Golgibodies 4. Endoplasmic reticulum Chloroplasts may be obtained ina relatively pure form by differential contrifugation of the cell homogenate for 2 minutes at a 100xeb. 12,000x2 ce. 1000xg 4. 144,000-x 9 The only modified base present in plant DNAis a, J-methyl adenosine b, T-methyl cytosine ©, S-methyl eytosine d_psendouridine Winch of the following characteristic is correlated with most monocots but not di- cots? a, absence of reticulate venation b. absence of vessels in stem ¢, presence of triangular protein bodies d._ presence of raphides ‘A plant genetic engineer wishes to transfer and express a gene horn sunflower into beans. Which of the following would be the vector of choice? a, Lambda phage b, pBR322 plasmid ©. Ti plasmid a. Maize streak virus Identify the compound whose structure is given below f enone 7 2" \ yt OW a, Abscissic atid b. Zeatin ©. gibberellin a. auxin ‘The theory of Ishand Biogeography was developed by a. Darwin b. Wallace ©. MacArthnar and Wilson d. None of the above Based on biogeographic evidence, the ‘Wallace line separates a, Australia from New Zealand b. Australia from Indonesia ©, Australia from Japan Sof le Japan fiom Indonesia What are the botanical names for the following economically important plants and to which family do these belonig to? a. Cotton, b, Rubber, © Coffee, 4d, Tobacco; ©, Cacao 6) Answer the following A. What are meristems? Mention briefly about the role of meristems in plant development. 3) }. Define the following Q) Sometic embryogenesis . partheriogenesis Answer the following : A How do cellulose, pectins and hemicelluloses differ in terms of G) structure (ii) solubility ® B, What is the main difference between maize transposons Acand Ds? (2) Answer the following : A. Desctibe briefly the “altemation of generations” as it applies to flowering plants. in Neurospora, a cross between senescent females with normal males gave all senescent progeny. A reciprocal cross of normal femiales with senescent males gave all normal progeny, What can you conclude about the senescent mutation? e) List any five developmental processes that are regulated by phytohormone auxin. (5) For each sub-question given below, four alternative answers are provided of which one or more are correct. Write the correct answer in the answer book by writing a, b, ¢ or d along with the corresponding sub-question number. (201 =20) A bacterin, having doubling time of 10 minutes, fills. a cylindrical vessel completely in 3 hours. How much time will i take to fill halfof the vessel? a. 80 minntesb, 90 minutes © 150 minutes d. 170 minutes The source of hydrogen for reduction of carbon dioxide in purple and green bacterial photosynthesis is a HO b. FADE: . NADH dan organic/inorganic compound Which of the following microorganisms are involved in decreasing soil fertility? ‘a. Pseudomonas acruginosa b. Bacillus denitrificans Nitrosomonas d. Azotobacter The antibiotic streptomycin was discovered by a. Alexander Fleming b. Robert. Koch Louis Pasteur dS. Waksman Which of the following vitamins is a precursor of coenzyme that is required in enzymatic reactions involving transfer of acyl groups? a. Lipoic acid b. Biotin © p-Aminobenzoic acid 4. Riboflavin The chemical nature of enveloped viruses ix \. Protein glycolipid lipoprotein I. nucleoprotein Blastospores in fungi are formed due to 4. binary fission b. budding ©. fragmentation sexual reproduction For isolation of which group of microorganisms, can agar-agar he used as a solidifying agent? |. organisms growing up to 30°C organisms growing between 30 - 50°C organisms growing between 50 70°C ‘organisms is growing above 90” C Dot 16 Mutuatistic association between fungi and roots of higher plants is known as a, Mycorthiza fh. Lichen Legumes 4, Corrratloid roots Alteraria solani causes a, Early blight of potato b. Late blight of potato © Rust of crucifers 4. Powdery mildew of pea ‘The major functions (features of the Golgi apparatus in encaryotic cells are a, Ttearriers out Cure glycosylation of the proteins being transported b. Its the major protein sorting centre of the cell ©. Te forms secretory granules jn its trans ‘compartment 4. Theonsists of lipid bilayer membrane Which one of the choices best completes time statement: The fact that DNA polymerase T from E. coli his a 5° -»3"-exonuclease activity implies that the enzyme has multiple subunits implies that DNA polymerase f can use both DNA and RNA as primers makes the enzyme able to detect thymine dimers in double-stranded DNA. enables the enzyme to phy all important sole in DNA replication ‘The following sequence best srranges (RNA, tRNA and DNA in onder of increasing molecular weights, a IRNA, DNA. FRNA b. IRNA. rRNA, DNA . RNA, RNA, DNA. 4. FRNA, DNA, tRNA Which of the following is the major function of the lymphoid system a inmate immunity acquired immunity inflammation 4. phagocytosis ‘That DNA is the genetic material can be evidenced by the fact thatchromosomes are made of DNA DNA jis net present in cytoplasm © transformation and transduction in bacteria are caused by DNA only DNA is concentrated in nucleus If the sequence of bases in one strand of DNA is ACGGGTTAT, the sequence of bases in the other strand will be a. TGCCCAATA b. GTAAACCGC & CGCCAAATG 4. ATAAcCCGY If the first base in the seoment of DNA. ACGTCGATCCCTATG, get mutated, its effect on coding by this segment will result in a, No change in the sequence of amino acids ‘one amino acid less in the protein A complete change in the sequence of many amino acids 4. A change of the first amino acid only For a double-stranded DNA molecule, which of the following statements is wrong? a. A/T ratio is constant 1b. Cis always equal to C & ASTCHe dh. (A 1 0)/ (C © T) ratio is cc Chlorine gas is used as a disinfectant in treating water supplies, it kills micro ‘organisms due to its action as a. alkylating agent b. oxidizing agent © protein precipitant lipid solvent Which one of the following is known as d. Anabaena Consider the Lactose operon of Escheriet coli and match the various genes | DNA sequence elements given in column 1 10 their function(s) | feature(s) given in column IL (3s1=5) Column I ‘A. promoter gene Wot t6 B, operator gene ©. CAP binding site D. Z gene E. igene Column IT 1. engodes a protein that interferes with the binding of RNA polymerase bound — specifically by = RNA. polymerase bound- specifically by lac repressor hound specifically by the cAMP - C. comptes 6, is aregulatory gene 7. encodes a aalactoside permease 8. encodes a protein that hinds allolactose Answer the following = A. What is the basis of tuberculin test’? How can persons suffering from tuberculosis be sereened by using this test? Interleukins have played an important role in the development of immune response How are they produced and what are their main functions which Jead to this property? Answer the following = A, Write down the Koch"s postulates which set certain criteria for providing the causal relationship between a microorganism and a specific disease. B. Give important features of bacteria that lack cell wall C. Why iy it not possible to culture viruses in cell-free medium 7 Answer the following = A. Why does growth of a microorganism decrease above its optimum growth temperature ay Escheribia coli can derive energy by using both fermentation as Well as anaerobic respiration depending on nutritional and environmental conditions. If sodium nitrate is added to.a culture of E. coli which is growing fermentati would it result in increased yield of the culture? Given reason m support of Your answer. (2) '. An inoculum of E. coli is prepared by growing it in a lactose medium, Whenthe culture is in its log phase of growth, it is used to inoculna dhwen tedium whieh contains glucose as the sole source of carbon and d instead of lactose (all other medium ingredients remain the same). Assume that all lactose was consumed at the item of tansfer of the inocalj to the new medium. Depict the nature of the prowth curve of the bacterial in the new medium. Q) Answer the following < A. Write down a complete reaction (with substrates, products, enzymes and coenzymes) of the glycolytic pathway Which exhibits substrate- level phosphorylation mode of energy generation. a) . Write down the first (the activation) step of the metabolic pathway used by sulfate-reducing bacteria (such as Desulfovibrio sulfodismaums) — in reducing sulfate to hydrogen sulfide. Q - Certain sulfate-reducing bacterial carry out disproportionation reaction for energy metabolism using sulfur compounds of intermediate oxidation state. Write down the chemical reaction used by the bacteria for disproportionation of sulfite for energy eeneration. 2) Define the following : a. Regulon Regulatory mutants ©, Calabolite repression 4, BOD © Biocontrol L1Z00LOG' Hot t6 ‘The invertebrate Peripatus is considered to be a primitive form of the following a, Mollusca hb. Annelida & Arthropoda a, Coelenterata ‘he correct statement amongst the following is 4. All invertebrates are also chordates b. All vertebrates are also chordates ©. All chordates are also vertebrates 4. Vertebrates and chordates are synonymous The term “Jurassic” in geological time scale refers to a Era b. Age Epoch a. Period ‘The compound central to most of the reaction pathways leading to the abiotic formation of simple organic compounds containing nitrogen is 4. Hydrogen evanide Twin birth occurs in humans, In tins context dizygotic twins a. share identical phenotypic and ‘genotypic traits b. do not share both phenotypic and genotypic traits share identical phenotypic traits 4. share identical genotypic traits Somatic recombination is commonly found und is important for bioogical function of 2 Hepatocytes b. Lymphocytes For each sub-question given below, four ©, Monocytes alternatives are provided of which one is a. Keratinocytes correct. Write the correct answer in the answer For fadiolabelling DNA with 32P using book hy writing a, b,c or d against the enzyme DNA-dependent DNA corresponding sub-question number. polymerase, one would use + (20x 1=20) a a dATP b. a PATP ce yYPATPa, 7 PdATP The prosthetic group in the respiratory pigment haemocyanin is a Tron b, Cobalt eZine 4. Copper ‘The organelle not containing nucleic acid Rough endoplasmic reticulum Nucleus Mitochondsia Golgi apparatus In mammals oogenesis is initiated during fetal development, hut the germ cells are: 4. Arrested at the Ist meiotic prophase b, Are capable of undergoing fertilization ©. Like diploid cells 4. Capable of undergoing mitosis With regard to two hormones, Chorionic gonadotrophin and luteinising hormone, the statement that is correct ts ‘They are structurally identical ‘They have similar biological activity ‘They are produced by anterior pituitary gland Fach of them is made up of a single polypeptide chain At any given time, the veins of the human body hold about the following percentage of total blood volume a 25% b. 50% & 75% 4. 15% The maximum number of antigen molecules a single 14G ean hind is at 2 ad. 10 The causative organism of sleeping sickness is \. ‘Trypanosoma Wuchereria Ascaris I. Leishmanid 120616 It is possible to obtain an embryo. by transplanting donor nucleus to a recipient enucleated ooeyte. Ifthe donor nucleus is from sheep “A” and the recipient ooeye ix from sheep “B, the offspring bom out of this embryo is Genetically identical to only sheep “A bb. Genetically identical to only sheep B Share penetic traits of both A and B 4. Have genetic trait totally different rom sheep A and sheep B ‘The momphogen required for mesoderm induction during xenopus development is a, Albumin b. Inhibin Vitetlogenin Activin A water body near agricultural fields contains aquatic plants, herbivorous. and eamivorous fishes and camivorous birds, ‘The farmers regularly use pesticides in the field, In. which organism is the concentration of pesticide likely to be the highest? a. Camivorous birds b. Camivorous fishes €. Hexbivorous fishes 4. Aquatic plant All organisms including fauna, flora and microorganistos found in a given arca are termed as a. Biomass b. Biota c Biome 4. Biosphere Honey bees communicate about the location of food through 8. Pheromones b. Songs © Dance 4. Touch ‘The most social group of mammals are a. Ungulates b. Camivores © Primates 4. Rodents ‘Match the pair of collaborating scientists from Column A arid B to their work in Column (Sx1=5)Monod Kohler Column 8. Monoclonal antibody b. Selfish DNA © Operon hypothesis d. DNA replication © oncogene Define the following = (Sx1=3) a. Dominance hierarchy b. Adaptive radiation © Apoptosis 4. Uricotelism e. Transgenic animals Answer the followin A. What are the differences between directional and stabilizing selection? Winch one produces evolutionary change? G8) “What are the differences between hemimetabotous and holometabolous metamorphosis? Q Answer the following A. Why is it believed that sitochondria was once a free living prokaryote which had established a symbiotic relationship with an ancient unicellular eukaryote which has been selected during evolution? @) Pictorially depict the cell cycle with all its phases. Name the major events that take place in each phase, ce Answer the following : ‘A, What is biogeochemical eyele ? B. Why would DNA with high G + C content need higher temperature to ‘melt as compared to DNA with high A + Teontent ? 2) 13 of 16 C. State the reasons why amino scids like Valine, leucine, Isoleucine, phenyl alanine and methionine are usually found intemally in a globular protein a) Answer the following A. Name the antibody molecular hat is present on the surface of virgin B- lymphocyte that functions as receptor for the antigen, How docs it differ form the secretory form 9 (2) ‘What is criss cross inheritance? (2) ‘Name the parasite that causes flaria and which are the endemic areas in India for the disease. a) For each sub-question given below, four alternatives are provided of which one is correct. Write the correct answer in the answer book by writing a, b, © or d against the corresponding sub-question number. (25x 1=25) Recombinant DNA human health care product. developed for treating hhacmophiliae is a Interferon b. Lymphokines ©. Coagulation factor IX 4. Phytohacmaglutinin Which of the following features of transcription is similar to that of replication’? a No primer is, required for polymerization b. Polymerization does not have proof reading acti Newly synthesized strand starts falling off the template before complete RNA is synthesized 4 RNA synthesis requires. DNA Topoisomerase action ‘Terminal transferase is used a. To add base at the 3° end of the DNA bb. Th add base at the 5° end of'the DNA ‘To carry out Nick TranslationTo transfer phosphate at the 3° end of the DNA, Which of the following processes in protein synthesis requires hydrolysis of GTP? a. Initiation b. Elongation ©. Termination d, Post translational modification Which of the following components is necessary for the synthesis of ribosome from RNA? 4 Ribosomes b. DNA polymerase ©. DNA ligase AL Topoisomerase Which of the following will not affect the expression of a clonod foreign gene host? a. Promoter strength by codon choive size of the host genome d. Plasmid copy number RNA ina DNA-RNA hybrid is digested by a Sy nuclease hest capacity vestor is Yeast integrative vector ‘Yeast artificial chromosome Cosmid Bacteriophage vector Production of secondary metabolites requires the use of 1. Protoplasts b. Apical Meristem ©. Auxiliary buds Cell suspension Resistance to herbicide glyphosate in transgenic plants is brought about by Overproduction of RNA polymerase. Undesproduction of EPSP synthase Overproduction of EPSP synthase Overproduction of Aspartate aminotransferase plants embryo rescue is adopted for overcoming 4. Histoincompatibilities 14 of 16 Prezygotie fertilization barriers Culture induced chromosomal abnormalities 4. Postzygotie fertilization barriers Cytoplasmic male sterility is encoded by a, Mitochondria b. Chloroplast ©. Cytoxol a. Ribosomes ‘The C Value denotes the total amount of DNA ing a, Ancuploid b. Diploid ©. Haploid 4. Polyploid Aminopterine is used during the production of hybridoma cells because a. It blocks the salvage pathway b. Itprevents the growth of B cells It prevents the growth of myeloma cells 4. Itblocks the synthesis of Ie by B cells ‘Salk and Sabin polio vaccines are 8. Prepared from two different strains of polio virus Both are attenuated forms of vaccines Salk vaccine is attenuated form and Sabin vaccine is inactivated form of vaccines Salk vaccine is inactivated form and Sabin vaccine is atlenuated form of vaccines Which of the following methods can determine the concentration of Hapten ? a. Rocket immunoelectrophoresis b. ELISA Ouchterlony immunodiffusion d. None of the above ede mutants are usefil for the study of 4, Chromosome breakpoint b. Apoptosis only © Various stages of cell cycle 4. homeodomain Which of the following cytokines are produced both by Ty and Ti2 cells ? a. IBN:d. IL-4 Most Industrial Enzymes are obtained fom a, Plants b, Animal tissues ©. Microbes d, Insects: In the conversion of Glucose to Fructose which of the following immobilized enzymes is used in the industry a, amylase b. Lactase © Ghicoamylase 4. Glncose Isomerase For a turbine aeration-agitation unit the Power consumption is the same for gassed and ungassed systems increases with decreasing furbine diameter :. is smaller for gassed system than for ungassed system is greater for gassed system than for ungassed system Industrial production of Citric Acid requires a. Oxygen, sugars and Saccharomyces cerevisiae b, Oxygen, Sugars and Escherichia eoli © Oxygen, sugars and Aspergillus miger Oxygen, Sugars and Acetobacter suboxydans Kia can be measured by sulfite Oxidation Method, The dissolved oxygen concentration measured by dissolved oxygen probe in a sodinm sulfite solution will be a, Zero b. Low © High 4. Vanable Which of the following can be used to count the viable number of cells a. Plate count b. Turbidity c, Dry weight measurement d. Coulter Counter Which of the following is tme for ‘Chemostat 15 0f 16 Concentration of limiting substrate is ‘maintained constant High productivity ean not be achieved Automation is difficult ‘Mutation is less likely to occur Answer the following : A. Protein X has a mol wt. of 30,000 daltons, Assuming average mol wt of an amino acid is 120 daltons, how ‘many codons are found in the mRNA that codes for this protein (include start and stop codons also) a) Explain the following observations in one or two sentences. Restriction enzyme 1(REL) acts at all sites acted upon by Restriction enzymes 2(RE2), yet RE2 can act only at some of the Sites acted upon by REL a) With the help of a line diagsam given the basic stmeture of a prokaryotic gene including ity 5° regulatory sequences and 3° termination sequence. Label each of the components a In the original PCR. (polymterase chain reaction) protocal Klenow —DNa polymerase was used which was subsequently replaced by’ thermostable DNA polymerases, What is the advantage of the latter ? Explain in 1-2 sentences. @ Following is a schematic representation of the autoradiograph of a DNA sequencing gel obtained with ‘the chain termination method, What is the sequence of the DNA fiagment sequenced ? a A GT 27. Answer the following A. List different methods for DNA delivery in plant cells, a) B. What do you understand by the following. ? Give one example of each 2Plantibodies Plant vaccines Bioplastics |. Plants as bioreactors Describe in one sentence why bacterial gene gets poorly expressed in transgenic plants. a Describe in one sentence the utility of micropropagation technique in crop improvement. (WD Answer the following A. Match the items in Column A. with those in Column B Column A 1, Cell line 2. Hela cells 3. CHO cells 4. Primary cells Column B Can grow in suspension culture Undergoes senescence and death Can grow indefinitely in culture |. Needs solid surface for growth . Cyclosporine A is added while immortalizing B-lymphocytes with Epstein Barr, Virus Why? Why glutamine is an important component of animal cell culture medium’ ). What is the risk involved in using retroviral vector in making animal transgene? Answer the following : A. No amino acid side chain is a good electron acceptor yet many enzymes, such as Carboxypeptidase A, catalyze reactions in Which enzyme serves as an 16 ot 16 electron acceptor, How is this accomplished? a Can competitive inhibitor be an allosteric regulator? Explain in one sentence, a An enzyme is active when reduced, but loses its activity when it is treated with iodoacetamide, What amino acid is likely to be responsible for its activity? ay D, List out three different methods of immobilization of enzymes. ay E, List Wo factors which load to decrease in microbial activity in Ethanol fermentation. ay Answer the following = A. Derive a simple equation characterizing the self regulating capacity of a Continuous Flow Fermentation process. (=D). (1) A bacteria grows on glucose with flay = 05 Wh! The Ky with respect to ghicose is 0.05 kg/m*, What will be the specific growth rate of the bacteria When glucose concentration — is 0.3kg/m‘? mM List two techniques for disintegration of microbial cells a) Calculate the doubling time of an ‘organism whose specifi growth rate is Onn a Assuming that cells convert two thirds (W'W) of the substrate carbon (glucose) to biomass calculate the stoichiometric coefficient (ffi only) for ghicose utilization, a C05 + oO. + aQNHy > By (CagHz sNog6O) 2) + Bp CO3 = (hy HO Biomass)