FAST

EFFICIENT
E. COLI
TESTING

Solus E. coli O157 ELISA

Assay and Automated Workflow Package
 EFFICIENT TESTING: TEST MULTIPLE MATRICES
18 HOURS TO RESULTS WITH A SINGLE ASSAY

E. coli testing the simple, efficient,

cost-effective way
Efficiency Made Simple
For food testing laboratories everywhere, our Solus E. coli
O157 ELISA pathogen test kit is an easy-to-use, cost-effective,
and efficient method for the detection of E. coli O157 in food
and environmental samples.
Presented in an immunoassay format, the Solus E. coli O157
Solus E. coli O157 ELISA Kit
ELISA kit is versatile and can be used manually or in conjunction
with automation. Laboratories with higher sample throughput
can use the Dynex DS2 automated solution to improve laboratory Solus E. coli O157 ELISA Benefits
workflow, significantly reducing technician time and increasing
the capacity for testing. Cost effective
• Increased laboratory efficiency
The smart way to test for E. coli • Reduced demand on resources

With our Solus E. coli O157 ELISA kit, you have a fast, • Reduced waste
sensitive immunoassay that delivers results in about 18 hours
Efficient
– with 2 hours to presumptive negative/positive results after
• High efficiency when automated
selection enrichment. In addition, our Solus solutions can
make your lab more efficient and cost effective – and your • Significantly reduced technician time
results more reliable than ever before.
Reliable
• Independent validation demonstrates
high levels of sensitivity and specificity

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 HIGH THROUGHPUT EASY TO IMPLEMENT,
FOR BETTER EFFICIENCY EASY TO USE

Solus E. coli O157 ELISA Workflow

Ordering information
Solus E. coli O157 ELISA automated
Product Code Product Description Pack Size
process is carried out on a Dynex DS2
instrument. A single DS2 processes EC-048S Solus E. coli O157 ELISA 96 wells
two 96-well plates simultaneously, MED 026-0.50 kg mTSB 500 g
generating results for up to 558 samples DS2000 DS2 Automation 1
in an 8-hour shift.
Once the instrument is loaded,
the technician can walk away for up Validated to ISO 16140 and approved for use by AFNOR.
Certificate number SOL 37/03-10/15.
to 2 hours during each run, providing
time to carry out other essential
laboratory tasks. Dynex DS2

Test Methodology

Solus Automated Process

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Sample

STEP 1 STEP 2 STEP 3 STEP 4 STEP 5 STEP 6

Add sample to mTSB + Transfer 1 ml culture Pipette 100 µl sample Wash plate and add Wash plate and add Add 100 µl of
novobiocin, mix, and incubate to boiling tube into wells (plus controls) 100 µl of conjugate 100 µl of substrate stop solution
Incubation: Boil: Incubation: Incubation: Incubation: Read Plate:
41.5 °C, 16-20 hrs. 85-100 °C, 15-20 min. 37 °C, 30 min. periods 37 °C, 30 min. periods ambient room temp.,10 min. 450 nm

Negative Control Positive Control Blank Well Sample wells Positive (After Substrate Addition) Positive (After Stop Solution Addition)

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 s t
o ce A u
Pr u s
t ed S ol
a
o m Solus A
uSolus
t Automated
Workflow Comparison: Solus E. coli O157 ELISA vs ISO 16654 Process Process
Solus Automated
Timescale Day 0
s A Day 1

o lu Solus A
1 2
ISO 16654
S So
Plate 50 ul
on to CT-SMAC
medium
100 ml 25 g food
Solus Automated lu Process
Incubate immuno- Incubate +ve or -ve result
in 225 ml
mTSB+n sA
41.5 °C 18-24 hrs.
then take sample
magnetic capture
& wash
37 °C 18-24 hrs. confirm

ut Plate 50 ul
on to second

100 ml Plate 50 ul om medium

Solus Automated Process Incubate +ve or -ve result

at
Incubate on to CT-SMAC
So
41.5 °C 6hrs. @ recommended confirm
medium
then take sample
immuno- lu Incubate ed +ve or -ve result
temp and time

sA
37 °C 18-24 hrs. confirm
magnetic capture
Solus Automated Process
& wash 1

1
ut Plate 50 ul
2

2
on to second
om
3

3
medium

Solus Automated at Process

4

4
Incubate
+ve or -ve result
5

@ recommended
confirm
ed
6

temp and time

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Pr
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oc
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Solus E. coli
0 m 25 g food
10 11

10 11

10 es
O157 ELISA in 225 ml
mTSB+n

s
12

12

Incubate Take 1-2 ml heat

41.5 °C 16-20 hrs. kill, and test

Report +ve result

-ve confirm
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Solus Automated Process
Solus Automated Process
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For sales and support contacts, please visit www.solusscientific.com 10
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PerkinElmer, Inc.
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940 Winter Street

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Waltham, MA 02451 USA

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P: (800) 762-4000 or
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(+1) 203-925-4602
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www.perkinelmer.com
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For a complete listing of our global offices, visit www.perkinelmer.com/ContactUs

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Copyright ©2020, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.

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