4, Macao JLH., Jinumasos 10.B., Tpoce Pl. sap. // Becrunk apwtaox0rin, 2002, Ne 28. C. 67-78. 6, Mensuuixosa E.B., Jlauxun B.3., 3euxow HK. 1 ap. // Oxucaurensauii crpecc. Mpooxcuaairas w axTi 5, Maczoe JLH., JTaumwanop 10.B., Cwarun TH. // oxcnaanres. M.: C:roso, 2006. 556 c. Scmepuns. Kau. bapwannsiorUs, 2002, Ne2, C. 70-75. aoo0 Bucoxoodypexrupiias axixKocrias xponarorpadpiin (B92KX) p wacroamee ppens stmaseres speapwalitio TepenexTHBHM MeTOROM, NOSBOAAOLIS B OCTATON- ofl crenenit HateRHO HecTenONAT» 3aKOHOMEpHOCTH yaepxunania tpyanoxeTysHx H TepMomaGiLADIX cO- eaunennit, Taxne nccnerosanna mpuo6peraot ocoGoe stfavenie pM anrause Gxloxorsveckix OGveKTOD 1 GHo- sroruveckit axtipibtx wemtecrs. K nlocaeamia oTHocsT= ‘ca chaaponionans, conepaanitecs 8 teKapcraeHtiisx pac- ‘Tenia, Takwe KaK AMTHApoKBepKeTHH (/LKB) i HTH poxeseripepot (/AK@), ru coenmmenns oGnaaNOT un ‘Pokwnw cnlekrpom Gitostoruvieckolt akritBHOCTH, BKTIOAS ANTHOKCHLTAHTHYTO, MpoTHBOOMyxoneny!O, MpoTHBOAT- “Teprivieckyio, ipoTiBoBOCnaZiTEBHYD, KANHAASPOYE- pertanionty1o, renaronpotexropnyio [4 6,7, 10-13] mp. (Onenika conepsxanna cbrapononon BaKCTpaKTax 7eKap- Ccroenminx pacresmtit NogBOAAeT NOYES WeHHy!O HH- cbopaauumo orHocuTe:IBHO MepcmeKTHB HetTomBOOBAHHA pacrirresHoro ckpsst 8 ¢bapsaayeTpHat. ‘Here paGome — orrusarsanuts yeropuil aassa ra- sonowt08 AKB u JUK® ¢ rooms BOIKX wt kosue- ‘cTpenias onenka Wx conepacaHa B 9KeTpAKTAX JleKap- ccrwenmnrx pacrenil Marepuams 1 sevonst OGvexrann nayseniia eayaknm aKeTpAKTEX neKap- crmenirx pactenui, sxoasmuex B mpomien npenaparon ‘TuGercxoll Meauunes [5]: naTumcTanKa KycrapHHtKO- ‘oro (IIK) (Penthaphylloides fruticosa), Gana toxcT0- amcruoro (BT) (Bergenia crassifolia), ropeyasunka Go- poxatoro ('B) (Gentianopsis barbata), uztesnunxa 6aii- Kazsckoro (IIIB) (Scutellaria baicalensis), sonia ayGnenocnoro (3K) (Phlomis tuberosa), xeBscia Bet- coxoro (1B) (Inula helenium), unnopiita naypexoro (CULA) (Rosa dahurica Pall), :1enoxs xpyumnnosnanofi YAK 615.451.16.001.8 ILB. IpaxenaaGaes, A.A. jlytos, B.C. Xsoumeryen, C.M. Huxonaes, A.B. Canun OIEHKA COXEPKAHHA OIABOHOU OB B OKCTPAKTAX JIEKAPCTBEHHDIX PACTEHHIi METOJIOM BO2KX umunexaa zocydapemeesnan ueduyucran axadesus, 2. Yuma Peawme B paGore npesraraeres werozynca omoupentenoro Ko- suvectnetmoro onpexesemus duraponot.20n aarwIpoxxepte- ‘Tua w unIpoxesendpepona c mommy oGpameuo-astolt mucoxondpdpextunnoll xwixocruoli xposatorpaduot 9KcT- ‘parrax aexaperemcx pacremul. Toxasano npewymects0 ‘MeToaa mo cpansiemmno c oncammunar B aurTepaType. P.B, Tsydendambaev, A.A. Dutov, B.S. Khyshiktuev, ‘SIM. Nikolaev, A.V. Savin HPLC ANALYSIS OF THE FLAVONOIDS IN EXTRACTS OF MEDICINAL PLANTS. (Chita State Medical Academy, Chita Summary “The objective ofthe paperis to show synchronous quan- titative determination technique of dihydroquereetin dinydrokempferol flavonoids in the extracts of medicinal plants with reversed-phase high-performance liquid chro- ‘matography. The advantages of this technique compared with all previously described ones are presented in the pa per. (OK) (Hippophae rhanmoides) 1 noaopoxinnxa Gos ‘oro (IIB) (Plantago majoris). Crannapt JIKB (wacconas 2028 ne menice 99,0%, co- inyrtermyimtero chranoxowza suTitpoxenmpepota He 6o- sree 5,7%) npenocranten OOO HILITH “Buotexnpom’, (¢. Mocksa). Basontut pacrsop craniaapra (10 mt/sia) ro0- ‘Topicta na 0,01 M chocrparnom 6ycbepe (pH 7,49), paGo- ‘stil nosysazn pasGantenwen Gas0B0ro 10 Koute!TpALItConepacaume KB u KD ‘s axerpasrax aexaperaeminx pa, wxs/ (Mm) Decry KB AKO 6 13597, 02146 01249 16 OTT 192207 OK 2698/4292 163209 15 20054285 aoe ma siasse69 | 668203 ar. 1052,12109) 489206 2 Teepe 116205 3K 248587 576202 mK 1597273, 11205 100 nar /sex [18 nocrpoetnts KasmuGpoBosmtoit xpHBOt T0- ‘TopiLTH pactaopsi cravizapra ot 10 x0 100 sexr/tt. Si- ranterarite 9xctpaxr pacreni, honyesse 8 1a60p3- Topint Hicrirryra oGuielt n axcneprstenrrarsnoit Guox0- runt CO PAH (r, Yaan-Va), pacroopam now ae 6ytbe- pe (10 mr/2s1), O6eM saoaHMoro B eT MiEKEKTOPA Ccrartxapra — 2.01, npoGi— 10 4c. Anais npopOmET xa xponarorpaipe “Iria” (Anon), xonowxa 100 4 a € auracibep Cy, 5, 5000 TT (“ButoXsoMax”, 1. Mock a) + mpenkononKacC,, 43.0 (“Phenomenex”, USA), YO-nerexsusa np 290 1, smoeH aneroRIeTpHEA — 0.0025 M NaHLPC, (pH 3,0) — irsompontation (30 : 69 : 1 v/v), ‘exopocre noroxa 600 snca/sans, xannexne 40 Gap. Mapa- _Metputicnomayemrx bocrparHoro 6ythepa H MoGTKOtt «aati (woaapnocrs, pH, cooruouente) omperererts akc- epaMenTabitbin MyTeM, pit KOTOPHIX PerweeTpHpORAAH MaKcHMAIBHYTO ToNHOCTE HW MEaTBHOe CooTHOMENE coma /mym, 70 nlospomtao yaryanntT® pees eveKuHH ompeneasemoro nemecrna 110 cpabitenitio ¢ Meronanit, “omitcanrama 9 aiteparype [2, 9]. Las pernecrpaivtxpoMatorpasar ienomssonaica npo- rpamaio-annaparnuifi Kownaexe “Multichrom for Windows” (*Anmepcena”, Poccis). Conocrasene npe- ‘Meu yaepsRiBanns MHHKoD Ha xposaTorpanoe scmsTye- ahix OBpaation c BpeMenten Yepacnpamin Crarixapra no- Sv0mIUI0 WleHTHKpULEHPOBATE B HEX HcKOMBe BeMecTBa. Perucrpyposam mrkn paccanrramant xonerTpatino AKB w AKO p axcrpaxrax no neicore nikon cranimapTa. Bpes ynepacipanst nirkos crasitapra (200 nr): LKB — 3,390.2 som, JIK® ~ 5,1240,2 stint aitcora matka KB = 19,7409 wB, JUK® — 1,6620,6 mB. Bpesta yaepausa- sus maxa JIKB mpo6 — 3.4320,2 samt, AIK — 5,2520,2 ‘ami, Buicota InukOB paatiNsan B SaBHICHMOCTHE OT cone ants rapoHoWo8 B npoGax. Lupita nitKoB HeKOSEIX emlecra cranaapra mpoGw cocraBitxa 0,4+0,03 watt. Tipenen aerexnt — 10:ur/aut (curnan/tmyse~3). Vismepene xaxvioro o6pastia npowosut narruxpar Ho, Ananua Tony¥eHHMX AaNIBIx MpoBereH MeToAOM apwannoHnolt crarHcrHKGt, Peaymsranat w o6cyaczemme Pane coaepxanue ¢)aanoKouz08 necaexoBatn pas nvsmenvat MeToxamt [2, 9}. Cnoco6 H.B. BockoGoftim- xopoit nt coasr. (1992) meer cnesyiome nexocrarxst [2] 1) mexocrarouno ypcTarreseHt 3a cer TOrO, WTO B xasecTBe O1H0TO Hs KOMTONEHTOB 37IEHTA HCHO IYOT Bonn pacrBop ykcycHolt kucroTH (70% no oGxemy), xoTopitit .xanaer BiIcoKOH onTHYecKoit NxOTHOCTENO IE _ypesituntnaet cooTHomesHe CHIFHa/ityM, 47o kitanaeT ‘itioxenne Tostiocri onpeaeaettist JUKB; 2) amrrensHocT® Mponenentia axazmtsa 3a cueT Toro, ‘yro ykasanutail KoMMOHetT Ypem¢naer BASKOCTD 9m10- e11Ta, 470, 8 CBOW OHePEAB, NPUDOAIT K ypEAHeHINO Bpe- Ment antantsa, KPoMe TOTO, cHitxKaeTeR CcpOK cayx6ut KOMIOMKIt 3a CHET CoMAHIA BLICOKOTO NaBEHHS NAHM KownowentoM azioenTa. Cnoco6 E.II. Xpamovoii (2004) 3a c¥er neoGxonHMocTH axcrparuposaxiia /IKB na KoH- ewrpupyiomten narpove “JIuranax C16” yamtser npe- 1s uecaegonants [9]. Kpose sroro, MeTox wexocraro%- No yBcrattrenen, Tax KaK B KaNECTBe SaIDeHTa MpHMe- eves 48% werani,ricrosTasoBanTe KoTOPOTO OOyCTOB- _Tiwaer mupoxte risks [8], #7o cymtecTBeHO saTpYsIs- ler pacers xomuentrpanni H ciirxaer TosHOCTb OMpeste- srexnta. B jowoaenne, mpi HcmomTssoBaNHH TORBHAHOH ‘asst ¢ Goxrbumim conepxanem MeTaHOxAa Ka KONOHKE ¢ alaMeTpom 2 Mm peaKo YReHHBAeTER TARTEHHe, TO neGzaronpuario reficrayer tia KonomKy, yMebUIaA CPOK ¢ cayrx6u Tipeaaraewnsit cnoco6 orayaeres oF suumeyKasat- ais Tes, TO Las antaTisa MpMeNeTCA sTiLTaMETATIA KCTPAKT PACTeMHA, 4TO NORAAUET NAAVSHTS IKCTPAKTH ‘ocoGoit snicTors 1 axastifTesbHO MpOAITITTS CpOK SKCILAYa- ‘Tauuin KononKu, B ramen cnocoBe » xavecTne amoettra cniomssopatca eNO noxOGHEKi pacrsop, Tak Kak: a) mpusenenne anetomerpitta nosnonsier nosy‘aT» BB OKHE H YSKHe MG, 70 NOBBAMTAeT SYBCTBHTENDHOCTE H ‘TownocTs crocoGa, a MeraHa OGyCrORIBAET tH*pOKIE “paswrurrete” mnt 6) wsonponanon mpunesneres BKae- cerpe craGiumsaTopa EpeMeti yaepsxHBaHIL, TAK KAK 4eM ccraGvixoitee apes yepaxtinanits, Test cnet tuHee WteH- Tuitkanmia nemecraa. Tarun OGpasom, mpextaraemt en10co6 simxseres Gone Toss, GrICTpH, HeTpya}0eM- 1M 1 OG.TaMAeT BHICOKOII BOCTPOHBOANOCTND. Tpeanoxeriin eToxoM onpeaeztena KOHHeHTpanit ‘raporion.zon 8 usy-uaentx akcTpakrax (TaGrtta). Peay.Abe ‘Tart oxcriepiietrTon nokase.n, 0 sHa‘HTeMBHBIM CORED sxaunest JUKB ormeaotes axcrpaxrat HB 1 TB (13,5 1 4,94 str/r coorvereTpen0). Mestne scero UKB oGwapy- “xenon axerparrax ITK u 3K. To xomectsy [1K sue Pyromyio rosmMo saKHMAeT ZIeNOTK GafiKaTSCKO — 420 wxr/r, a Taroke Gavtat ~ niosra 49 MKr/t aRCTpaKTA. Minmnnsatbae savers storo WoxasaTea saperncrpH- ponaitty LIL it 3K, Bocnpoxtssomueocrs citocoGa ouesit- ‘BUI TO srayenHo Koogypunnenra napraxitn (V): V=(S/M) 100%, rae S — cpeanexsaaparieckoe orkxonenne, M — cpeniiaa apnderisieckas. Koadyputnent papnarut (V) ‘rroco6a cocraBiia 9,1%, ‘Maerores santie 0 renaronporexroptioll, Mext6pa- noctabittoupyiomtell 4 arrrHoxitcairressHolt axrHBHO- ev IIIB [1, 3], oenony Koropitx, xpome npowts, Moxer coctannstT» BiicoKoe conepaKartite GhxapoHOHLAOB. Bumoxst 1. PaspaGoran Guicrpeii, ocTarosno BocnponsBaatt- Musi meron xommmectoennoro onpeneennia KB nAKD -b grusianeraTibrx oxcTpaxax sekapcTBeHtax pacretttil, oTopiti MoxeT Oho NeTIOTESONAH w Hex CralLLapTH- a mpenapaToo na OcHone aux GyaBOHONAOR. 2. Mpextoxentism MetoxoM otetteno conepxarttic JAKB 1 AK@ p noysaeanx oGpasnax. HauGonee Gorana akeTpaKrii unremaKa GailxatbcKoro H ropesaBitn- ¥a Gopoxaroro. Jurepatypa 1. Axxytona T.A. // Ax. mpo6a. cbapmaxoz. w nowcKa ux eKapero. mpenapatos. Tomek, 1987. C. 70-72. 2. BockoGoiiimxosa HB. Teoaaxas C.B., Troxapkit- na H.A. wap. // Dapania, 1992. 26, C, 74-75. 3. TomwaGepr E. JL, Jisralt A. M., Jeraunenko B. H. ap. ULnenannx Gaftxanscxati:dirroxnaci at cbapmano- ormueckne cnojicrsa. Tock: H31-no Tomek. yii-ra, 1994, 223 c, 4. Komxup B.K., Toxapkima H.A., Busxos B.A. 1 2p. // Xv. apne akypwas. 1995. 9. C. 61-64, 5. Mar-nt 10 sisy'exino TpARMUNOKKOH citcreNts nngo-ruGercKoit mennnitust. HopocnOupex: Hayka, 1982, 102 ¢. 1 6. Tapacosa E.A. // Tiparrwseckas ¢rrotepamus 1999, Nel. C. 37-41 7. Troxapkia H.A., Py.tenko H.A., Konecnitk 10.4. // Bonpocss murranta, 1997. N26. C. 12-15. 8. Xeqpraant 3. Xpowarorpachiia: mpaxtiseckoe mpi roxetne metona / Tlep. ¢ aura. M: Mup, 1986. 9, Xpamona E.IT // Anamrixa CrOupit it Jlatbnero Bocroxa - 2004: Mar-nsx VII xontp. C. 154. 10. Bronner C., Landry Y. // Agents Actions. 1985. Vol. 16 (3-4), P. 147-151. 11.ChuS.C, Hsieh Y.S, Lin J.Y. //J.Nat, Prod. 1992. Vol. 55 (2), P. 179-183, 12. Kandaswami C., Petkins E., Drzewiecki G. etal. // Anticancer Drugs. 1992. Vol. 3 (5), P. 525-530. 13, Schwarz A. Middleton : Immunopharmacology. 1984. Vol. 7(2). P. 115-126. 900 YAK 612397811 TH. YepnoGemcxuit, O.H. Hosoxpememnil, 0.A. JleGeasto, E.H. Casonona CO3AHHE ®YHKIMOHAJIBHBIX MACHBIX TIPOAYKTOB C YCHJIEHHBIMH AHTHOKCHJAHTHBIMH CBOHMCTBAMH Alarnesocmooit zocybapemaennazo meduyunceuit ynusepcumem; 000 “TPHT Yepuobexvcxo20”, z. Xa6apoock Cosnane ynkunonanbitsx nponykTos sBaseres axiiefimun HaNpansleHMeM KYTPHIIMOOTHN, OSDOAR- ‘ouui pemmirrs npoG.tenry zncGanancon B pautnorie conpe- ennoro sexonexa, OszopanamBaionute MpOYKTH ¢ 32- ania cpoiicreann ory Gorre ypexrMoHN cpes- THAME Hlony.ARUMOKHO! MpodpwLtaKTHKH HaMGonee co- uya.nbHo siaunoeix narorori (7, 16]. avtee a cooGua.nk o buartyeke cepin cpymkunoHa.ID- Hix MCHA NPoAYKTOR ¢ MlOWICKEHMENM cONepAHMEM ‘xutpa w xoxecrepina A213 nepawsnolt w propiinoli mpo- ‘uviaxrinat atepocicteposa [9]. Cymectnenitia aneH0M MHOrHx naTOOrHMeckitx COCTOaHHI MenOveKA, BK MONA Peaxitio opranitsma Ha HeGraronpustruee axropst yeumnedi cpeans, tasieTcs oxcutarupeetcrpece. B kot- Hike IIMpOKO HeosbayloTes sleKapcTnenHBLe epexCTRA ‘antoxcinanTHoro aelicraus, Buecre ¢ Tex, peatatoc- ‘Tob craHopwTes HocTynmenne B opranuaM cymecrbeH- Noro KomtuecTEa cBoGomNNX pAIHKAIOB ¢ NPORYKTaNH itTeabHoro xpanentia. MspectHo, sro xmtTexbHoe xpa- ene 3aMopoxkeHHOTO MUICA MpMBOAMT K SHAUHTETEHO- My NopéeHitio KoNHIeHTpalINK MpOAYKTOB NepeKsIcHO- Fo oxncaenns sumtnos (TOI) [13, 15]. Haxonrenne mpoaykron TIOJI smaxeres octiosnoii mpxssmmofi nops- mena skecrkocTHt Gena mpu xpartennt [10]. JL ci 2xeHNAL MITTEHCHBHOCTH MpowteccoB NepoKcHAaIUNM Mit [BKOTOMILIH B PetLeMTypY HOBLIX paspaarimpaennx Miic- ‘uBIx MpOAYKTOB GHOAOTHYECKH AKTHBHYIO MIMLeByI 20- Gapky cexeiitr Marpita, a Tarcke HCTONHNK MpMpoAHX ‘Toxorhepo.tos — opex Keapopiti. He-tio npoponunoro ccrexOBaHia GbL10 COMOCTABIeHHe AKTHOKCHAHTHNS cwolicrs HoBiIx mpoykroe o cucTene in vitro. ‘Marepuai 0 MeToas Ocymecransnm aaa nponecco ceobonHopaan- ambHOro OKNICAEMNS D CCeAYeMBIX MpoAyKTAX MeTO- Row xeanimoumnecttentm (XMII). Persicrpattino XML ‘Mpowoai.an na mostectieTHOM chiektponerpe LS 50B “PERKIN ELMER’. Cranizapritsamino enriana u ware- Matiieckyio oGpa6orky Kpussix XMJI punoansan ¢ omouusto mporpasoass Finlab, Croonrantiyio 1 waitynupovamyio Fe XMII nceae- oat no weroay 10.A. Braniowposa 1 coast (1991). ‘Onpeaeasar: enerocyany sa 1 wen enonrantiolt XM (S,), neamrunna xoropoil Koppesmpyer c murTeHcHBHOC-