Laboratory Exercise No.

4 PREPARATION OF CULTURE MEDIA

RESULTS

(In paragraph form, describe the appearance of the prepared tubes

and plates. Provide any illustrations of your results.)
Questions:

Short but concise answers must be placed after every

question. Limit your answer to 5-7 sentences.

Cite your references (as well as in-text citations).

1. List the factors that will guide you in choosing what media to
prepare.

2. Why do we always use distilled water instead of tap water in

dissolving our media?

Tap water should not be used as it contains impurities such as

calcium and magnesium and their metal ion traces. In addition
chlorine and fluorine will alter the characteristics of selective media.
(Thermo Scientific)

3. What are the problems encountered in media sterilization?

What could have been the cause of such problem?

4. Classify the different culture media according to:

a. Composition

b. Consistency

c. Manner of preparation

d. Function

REFERENCES
CABRI Consortium. (1998). Appendix I: Preparation and Storage of
Culture Media. http://www.cabri.org/guidelines/micro-
organisms/M203Ap1.html

Thermo Scientific (n.d.) Technical Support-FAQs.

http://www.oxoid.com/UK/blue/techsupport/its.asp?
itsp=faq&faq=tsfaq009&cat=culture+media
%2C+supplements+and+raw+materials&lang=EN&c=UK