National Institute of Virology

Dr. Panjwani Center For Molecular Medicine and Drug Research

International Center For Chemical and Biological Sciences
University of Karachi

Name : Ifza S/O, W/O, D/O : M Ali CNIC : 4220114794794

Age / Gender : 17 / Female MR / Lab No : 45449 Sample Received : 23.09.2020
Reporting Date : 25.09.2020 Referred By : Const. Virologist : Dr. Muhammad Rashid

Result : SARS-COV-2 RNA (COVID-19) " NEGATIVE "

Test Name : SARS-COV-2 (COVID-19) RNA DETECTION BY PCR (QUALITATIVE)
Sample : Nasopharyngeal/Oropharyngeal Swab
Principle : The Real-Time PCR Coronavirus (COVID-19) assay is an in vitro diagnostic test based on
fluorescence-based detection of specific SARS-CoV-2 targets in the viral genome.

Method:
The test was performed after RNA extraction using automated RNA extraction machines and amplified using
real-time PCR machines (BioRad CFX-96/ABI QuantStudio 5). The following controls were included: RNA
extraction control, PCR positive, and negative controls.

The analytical sensitivity of this assay is 0.58 copies/µL.

Electronically verified on 25.09.2020 11:46 AM No Signature required.