Title: Spectrophotometric Analysis of Aspirin

Objective: To determine the amount of aspirin in a commercial aspirin product.

Results:
Mass of acetylsalicylic acid used: 401.3mg
Solution Concentration Absorbanc
(mg/L) e
A(1ml) 16.05 0.126
B(2ml) 32.10 0.275
C(3ml) 48.16 0.410
D(4ml) 64.21 0.565
E(5ml) 80.26 0.724

Unknown 19.11 0.178

solution

M1 = 401.3mg / 0.25L
      = 1605.2mg/L
M1V1 = M2V2 
(1605.2mg/L)(0.005L) = M2 (0.10L)
                                M2 = 80.26mg/L
The concentrations for B,C and D was obtained by substituting the value of V1 as 0.004L,
0.003L ,0.002L and 0.001L
 Absorbance versus Concentration
0.8
0.7 0.72
0.6
0.57
0.5
Absorbance

0.4 0.41
0.3 0.28
0.2
0.13
0.1
0
16.05 32.1 48.15 64.21 80.26
Concentration(mg/L)

Absorbance

From the graph plotted,

Molar absorptivity for aspirin at 530 nm, ε = 9.313x10-3cm-1ppm-1
A = ε l CAspirin
0.178 = 9.313x10-3cm-1ppm-1 x 1cm x CAspirin
CAspirin=19.11mg/L
Thus,
M1V1 = M2V2
M1(0.005L) = (19.11) (0.10L)
             M1 = 382.2mg/L
M1 = mass / volume
382.2mg/L = mass / 0.25L
Mass of acetylsalicylic acid in the commercial aspirin product: 95.55mg
Amount of aspirin in unknown solution: 95.55mg 
Accepted value: 100.00mg 
Percentage error: 4.45mg
Conclusion:
The amount of aspirin in a commercial aspirin product was successfully determined to be
95.55mg with a percentage error of 4.45mg.