SOP for Glassware Washing Standard operating procedore ocean the gaseware wih soap salion ncvsing cleaning of Ppaties and Bursts. 4.0 0BJECTIVE To describe the procedure for the procedure for glasswar washing. 2.08coPE ‘This SOP is applicable to the procedure for glassware washing 3.0 RESPONSIBILITY Officer! Executive - Quality Control 4.0 ACCOUNTABILITY Manages — Quality Control 5.0 PROCEDURE 15.1 Preparation of cleaning solutions For the cleaning of olassware, those are used for chemical analysis special precautions will ake place to avoid any chemical residus in glassware, hich may later interfere withthe results of chemical analysis. Following cleaning solutions were used for cleaning of glassware: ‘AJ 0.5% Labolene 3] 2% Liquid soap solution 5.205% Labolene 5.2.1 Measure § ml Labolene and add ito container containing 995 mi of putiied water and str it wel, For better results use hot water. 5.2.2 Use it for cleaning of laboratory glassware such as bollles, flasks, beakers, and tubing 5.2.3 Recor the solution preparation details in glassware cleaning solution preparation record 5.3 2% Liquid soap solution 5.3.1 Measure 20 mi soap solution and add itta container containing 980 ml of purified water and sit wall, 5.3.2 Use it when 0.5% Labolane is nt a lable then itcan be used for glassware clearing ‘5.9.3 Record the solution preparation details in glassware cleaning solution preparation recor, 5.4 General Cleaning 5.4.1 Cleaning of glassware, which has contained hazardous materials, must be solely undertaken by experienced personnel 5.4.2 Most new glassware is sight alkaline in reaction. For precision chemical tests, new glassware should be soaked several hours in aca water (1% solution hydrochloric aed or nite aca) before washing 5.4.3 Wash glassware as quickly as possible after use i it's nol possible then the articles should be allowed to soak in water. 5.4.4 For cleaning of glassware such as bottles, tasks, beakers, lest lubes, etc use 0.5% Labolene. For better results use hot water 5.4.5 For goneral cleaning 2% quid soap solution may also be used when Labolene are not availble 15.4.8 During the washing all paris of the article should be thoroughly scrubbed with a brush selected for the shape and size of the glassware. Brushes should alvays be in gooe condition to avoid any abrasion ofthe glassware 5.47 Special types of precipitate material may require removal with nic acid, aqua regla or fuming sulphuric acis. These are very corrosive ‘substances and should be used only when required. 5.4.8 Bofore cleaning of glassware remove the labeling of marker pon with the help of IPA or acetone. For plastic ware do nat use acetone for removing marker pen labeling 5.43 Its imperative that all soap detergents and other cleaning fhids be removed from glassware before use. This is especially important with dotorgents, sight traces of which wil interfere wih serological and culture reactions. After cleaning, thoroughly rinse with tap water ensuring that containers ars party ited with water, shaken and emptied several times. Finally rinse with purfed water. 5.4.10 After cleaning dy the glassware in Hot air oven at 60 °C temperature + 5 °C. 5.4.11 Aways protect clean glassware from dust by use of tomporary closures or by placing ina dust ree cabinet, 5.5 Cleaning of specific types of glassware 5.5.1 Pipettes 5.5.1.1 Place pipettes tips down, in a cylinder or tll jar containing disinfectant solution immediately after use 55.1.2 Do not drop them into the cylinder, since this may break or chp the tps and render the pipettes useless for accurate measurements, A pad of cotton or glass woo! atthe bottom ofthe cylinder wl help to prevent breaking ofthe tips. 5.5.1.3 Ata convenient time, the pipeties may then be drained and placed in a cylinder or jar of dissolved detergent or, exceptionally diy, in ajar of Chromic acid cleaning solution. 455.14 After soaking for soveral hours, or overnight, drain the pipattes and run tap water aver and through thom ntl all races ofa aro removed.‘Soak the pipettes in puree water fora least one hour 55.1.5 Remove from the puried water, dry outside witha cloth, shake out the water and dry in hot air oven, 5.5.1.6 After drying, place pipettes in a dust ree drawer 5.5.2 Burettes (with glass stopcock) 55.2.1 Remove the stopcock key and wash the burette with detergent and water. 5.5.2.2 Rinse with lap water unl all the dr removed, Then rinse with puted water and dry. 5.5.23 Wash the stopcock key separately. Before the stopcock kay is replaced inthe burett, lubricate the joint with @ small amount of lubrant. Remember that burette stopcock keys are not interchangeable. 5.5.24 Almays cover bureltes when notin use 6.0 ABBREVIATIONS 6.1 SOP - Standard Operating Procedure 6.2 IPA Isopropyl Alcohol ANNEXURE-1 GLASSWARE CLEANING SOLUTION PREPARATION RECORD. Name of glassware clearing solution Date of preparation Prepared by! Use befor: Checked by Method of preparation: Take. ml. purified wa in clean and dry 8.8. container or sutable and add lig of cleaning solution and mix wall Put label on container having details of name of cleaning solution, date of preparation, use before and prepared by. Ankur Chowtnan snc’ rat profess ‘sharmaceutial Blog since 2008. Sign-up forthe ree email updates Need! Hole: Ask Question ly 080 of pharm