Optimize Performance

and Maximize Efficiency

in Your Lab
Accessories and Supplies
for UV-Visible Spectroscopy
2
 Table of Contents

General Information ..........................................................................................4

Standard Cells ..................................................................................................10
Cylindrical Cells ................................................................................................15
Flow-through Cells ...........................................................................................16

Spacers ..............................................................................................................2l
Stirring Bars .......................................................................................................2l
Cleaning Fluid ...................................................................................................22

HP 8452A Spectrophotometer .......................................................................25

HP 8453 Spectrophotometer ..........................................................................25

Cell Holders .......................................................................................................26

Multicell Transport...........................................................................................26
Peltier Temperature Controller ......................................................................27
Autosampler .....................................................................................................27
Sipper System ..................................................................................................27

Cell Holder Supplies ........................................................................................28

Multicell Transport Supplies ..........................................................................28
Peltier Temperature Controller Supplies......................................................29
Autosampler Supplies .....................................................................................29
Sipper Supplies ................................................................................................29
Tubing and Fittings ...........................................................................................30
Dissolution Testing System Supplies ............................................................31

Checkout Samples ...........................................................................................32

Certified Calibration Standards .....................................................................32

HP revolutionized UV-Visible Spectroscopy in 1979 with the world’s first

diode-array spectrophotometer, the HP 8450A. Since then the popularity
of HP spectrophotometers has grown enormously and our users have
come to expect the highest standards for quality. Agilent Technologies is
fully committed to continue the tradition established by HP.

This guide to UV-Vis Spectroscopy accessories and supplies will help

you keep your system operating at peak performance. It provides a full
Visit our web site at: range of supplies with the same high quality standards as our spectro-
www.agilent.com/chem/uv photometers. Except for product specific supplies all cells and acces-
for the latest news about sories can be used with confidence with spectrophotometers from other
UV-visible Spectroscopy manufacturers.

3
 General Information

Quality
Being a world leader in the manufacture of high-precision
products we are committed to producing the highest possible
standards of quality. For many years our Quality Control
Systems have been devised and operated to meet the require-
ments of the international quality standards
ISO 9000-9004.

Material
Cells illustrated in this catalogue are manufactured from Quartz
Glass or Optical Glass. The most important criterion for the
choice of a particular type of glass is the spectral range for
which the cell is intended. The range of window materials is co-
lor coded, each different color denoting the spectral range of the
specific material. Colored logos are fused onto each cell, thus
indicating the spectral range over which the cell can be used.

We supply cells in two materials: Quartz Glass and Optical Glass

Quartz consists entirely of silicon dioxide (SiO2) and shows some

remarkable properties.
• Quartz displays a high transmittance for UV light down to
below 200 nm, the extent of transmittance at low wavelengths
depending on the degree of contamination by impurities.
• The thermal expansion of quartz is extremely low, its coeffi-
cient of thermal expansion being 6 x 10-7 K-1 between
20 °C and 300 °C.
• Quartz is chemically very resistant and maintains its shape up to
approximately 1000 °C.

The grade of quartz used for the cells is characterized by its high
purity and homogeneity. It is produced from a silicon compound and is
also called synthetic quartz.

4

Note: Plastic
In general we do not recom-
mend the use of disposable
plastic cells. If plastic cells are
used it is important not to use
”apertured” cells where there
is a possibility that part
of the light beam may pass
through the side walls of the
cuvette and not through the
sample. This will cause erro-
neous absorbance measure-
ments (also see Semi-
micro cells, page 11).
Transmittance
Transmission values for empty
cuvettes
General Information
We use quartz SUPRASIL from Heraeus Quarzglas GmbH, which
gives transmission values of > 80 % between 200 nm and 2500 nm
for an empty cell.
Optical Glass cells are lower in cost but may only be used for
measurements in the visible range of the spectrum. The glass
we use is Schott Glaswerke type UK 5 and is characterized by re-
latively low values of refractive index and dispersion. This crown
glass is made from exceptionally pure raw materials, which gives
an improved transmission in the near ultraviolet range. An empty
cell will give a transmittance of > 80 % between 320 nm and 2500
nm.
Note: Plastic
In general we do not recommend the use of disposable plastic
cells. If plastic cells are used it is important not to use ”apertu-
red” cells where there is a possibility that part
of the light beam may pass through the side walls of the cuvette
and not through the sample. This will cause erroneous absor-
bance measurements (also see Semi-
micro cells, page 11).
Transmission curves, taken using empty cells are shown in the
figure left. The relatively low maximum transmission values of
slightly better than 80 % are caused by reflection losses at the
four glass/air boundaries. The losses by reflection depend solely
on the refractive index of the material used and can be precisely
calculated for each wavelength.
For example, at a wavelength of 587.6 nm we obtain the following
values:
Window Material Refractive Index Reflection Losses Transmittance
SUPRASIL 1.458 13.2 % 86.8 %
UK 5 1.523 16.1 % 83.9 %
5
 General Information

The window thickness of the cells is 1.25 mm. As the absorption

in the material can be ignored the effect of the window thickness
on transmission is minimal.

When comparing transmission data, it is absolutely essential that

identical measuring conditions prevail. Should a measurement
with a clean, empty cell yield significantly higher transmission
values, it is likely that this is due to a measuring error.

Production Methods
Ever-increasing demands on the cells have led to continuous
development and improvement of the manufacturing processes.
Concerning our precision cells, the most important procedure
are:
• Polishing
• Ultrasonic Machining
• Fusion

Polishing: Due to the use of advanced machines and based on

the development of new procedures, we can offer parts with
polished surfaces meeting the highest standards.

The surfaces of the cell windows in particular fulfil two indepen-

dent criteria, freedom from surface imperfections and a maxi-
mum degree of surface flatness.

Ultrasonic Machining: Many cell types, especially flow-through

cells, require borings and cavities with highly complex shapes.

With ultrasonic machining, which resembles the spark erosion

procedure used in metal working, we possess a technology that
allows us to produce high-precision borings and cavities of
0.5 mm to 60 mm in the brittle glass material.

6
General Information

Precision cells demand production processes that have to meet

the following high standards:
• the individual components must be made with extremely tight
dimensional and angle tolerances
• the polished surfaces must be exceedingly plane and free from
scratches and holes
• the fusion technique used must on the one hand guarantee a perma-
nently stable connection of the parts while maintaining the toleran-
ces, and on the other it must ensure a high degree of resistance to
chemicals and extremes of temperature.

Nowadays nearly all cells are assembled by direct fusion. This techni-
que ideally fulfils all of these requirements. However, it also presup-
poses that the surfaces to be fused together be polished and possess
a flatness tolerance of less than 1 µm.

Since no adhesives whatsoever are employed for fusing the glass

parts, the seams display the same high resistance against chemicals
and increased temperatures as the solid glass itself.

Antireflection Coating: Whenever radiation passes

through the cell part of it is reflected by the exterior surfaces.
The overall transmission is reduced as a result of this reflected
portion by approximately 8 %. Vacuum evaporation of thin layers of a
suitable material can reduce these reflections, thereby giving a higher
transmission for the cell.

Our cells are supplied with a multilayer antireflection coating that

reduces reflection over a broad spectral range. Reduced reflectance
is at most 0.4 % between 440 nm and 650 nm. This coating is highly
adhesive and resistant to both abrasion and climatic influence.

Dimensions and Tolerances: The path length is a particularly impor-

tant parameter for some photometric applications.

7
 General Information

Please note the following data for light path tolerance

Material Path Length Tolerance
[mm] [mm]
Quartz 0.01 to 0.05 ± 0.001
Quartz 0.1 to 0.2 ± 0.005
Quartz 0.5 to 100 ± 0.01
Special Optical Glass 0.1 to 20 ± 0.01
Special Optical Glass 30 to 100 ± 0.02

Handling of Cells
Our precision cells are manufactured from glass and quartz and
possess all the advantages and disadvantages of this material.

We generally recommend to clean and dry the cells immediately

after use and to return them to their storage case.

Do not keep the cells in the open in a corrosive atmosphere, and

do not leave the polished windows in contact with liquid over
long periods of time. Both conditions could lead to formation of
deposits or stains and could render the cells useless.

To avoid scratches on the precision-polished windows, the cells

should never come into contact with objects made of hard mate-
rials like glass or metal.

• Care is required when inserting the cells into a metal cell

holder.
• When filling the cells with liquids using a pipette, never touch
the polished windows with the pipette.
• Never use metal tweezers or pliers for carrying or holding
the cells.

Cells sealed with stoppers may crack as a result of increased pressu-

re. The most common cause of a pressure increase is the expansion of
the liquid within the cell due to an increase in temperature.

8
 General Information

A temperature increase can be caused by:

• heat conducted from an exterior source
• a chemical reaction within the liquid
• radiation absorption within the liquid

You can avoid the destruction of the cell by too much pressure in the
following ways:
• Fill the cell just high enough so that the light beam can pass
through the liquid. The liquid can expand into the remaining
cell volume when its temperature increases.
• Should you fill the cell to the brim, put the stopper on loosely so
that the extra liquid can escape. Do not try to force the stopper
into place, as this will inevitably result in damage to the cell.
• Use stoppers with a pressure release capillary.

Please note that high pressure may destroy some other kind cells as
well. This occurs if the liquid contained is subjected extreme changes
in temperature, e.g. when working with cell anaerobic measurements
or if a filled cell is submerged in liquid nitrogen.

Cleaning of Cells
Quartz as well as the other optical glasses used for our cells are
highly resistant to chemicals. Only hydrofluoric acid (HF) will etch
the surface within a short period. Inversely, this means that with few
exceptions all alkaline and acidic solvents, including organic solvents
may be used for cleaning.

From this, the following general recommendation can be deduced:

• If you know the nature of the contaminating substance, use the
solvent in which the substance was dissolved in for cleaning.
• In general and also with regard to environmental aspects we re-
commend cleaning with an aqueous solution of a cell cleaning fluid.
See pages 22–24.

9
 Standard Cells

General Description
Standard cells are rectangular cells with standardised exterior
dimensions. The cells either have an open top or a fused cover
which can be sealed with a PTFE stopper. Some of the cells with
open tops are delivered with fitted lids.

Macro Cells
The macro cell, which is defined by DIN 58963 as a rectangular
cell with an inner width greater than 5 mm, has emerged as the
standard for photometry. The most widely used macro cell is a
rectangular cell with outer dimensions of 45 x 12.5 mm
(height x width). The length of the cell is dependent on the desi-
red path length.

Macro Cells with PTFE lid

Path Length External Internal Volume Part No. Part No.
[mm] Dimensions Dimensions [µl] Glass Quartz
H x W x D [mm] H x W [mm]
1 45 x 12.5 x 3.5 44.5 x 9.5 350 5063-6546 5061-3384
2 45 x 12.5 x 4.5 44.5 x 9.5 700 5063-6547 5061-3385
5 45 x 12.5 x 7.5 44.5 x 9.5 1750 5063-6548 5061-3386
10 45 x 12.5 x 12.5 44.5 x 9.5 3500 5063-6549 5061-3387
20 45 x 12.5 x 22.5 44.5 x 9.5 7000 5063-6551 5063-6553
50 45 x 12.5 x 52.5 44.5 x 9.5 17500 5063-6552 5063-6554
Matched pair, set of two:
10 45 x 12.5 x 12.5 44.5 x 9.5 3500 5063-6550 1000-0544

Macro Cells with PTFE stopper

Path Length External Internal Volume Part No. Part No.
[mm] Dimensions Dimensions [µl] Glass Quartz
H x W x D [mm] H x W [mm]
5 48 x 12.5 x 7.5 42 x 9.5 1750 5063-6555 5063-6557
10 48 x 12.5 x 12.5 42 x 9.5 3500 5063-6556 5062-2477

Note: Spacers are required for cells with outer depth less than 12.5 mm
in order to hold them securely in the cell holder.
See page 21.

10
 Standard Cells

Semi-micro Cells
Semi-micro and micro cells have an inner width of 4 mm to 2 mm.
The thickness of the base is 9 mm. All semi-micro and micro cells
are for use with spectrophotometers having a beam height (z) of
15 mm.

It is important for the successful operation of these cells that

the light passes only through the measurement chamber and
that no light passes through the side walls. Since the cross-sec-
tion of the measurement beam can vary significantly from one
instrument to another it is often impossible to get a good match
between cell aperture and beam size. To ensure that no light
passes through the side walls or bottom of cells these parts are
made of blackened glass. For very narrow aperture cells used
with narrow beam instruments it is also necessary to carefully
position the cell to match aperture position to beam position to
achieve best throughput.

Semi-micro Cells with PTFE lid

Path Length External Internal Volume Part No. Part No.
[mm] Dimensions Dimensions [µl] Glass Quartz
H x W x D [mm] H x W [mm]
10 45 x 12.5 x 12.5 41.8 x 2 700 5061-3391
10 45 x 12.5 x 12.5 36 x 4 1000 5063-6558 5063-6559

Semi-micro Cells with PTFE

stopper Path Length External Internal Volume Part No. Part No.
[mm] Dimensions Dimensions [µl] Glass Quartz
H x W x D [mm] H x W [mm]
10 46 x 12.5 x 12.5 37 x 4 1000 5063-6560 5063-6561

11
 Standard Cells

Ultra-micro Cells
Ultra-micro Cells – with PTFE stopper
These cells are specifically designed for use in the µl range (down
to 50 µl). These cells fit into any standard cell holder and have the
advantage of requiring much smaller sample volumes than stan-
dard cells. Conventional cell designs required several times the
theoretical filling volume to reach a certain filling height, due to
the sharp edges of the cell and the resultant capillary action. The
ultra-micro cells consist of a bottom section, a measuring cham-
ber, and a top section. Due to the unique design of the top section,
capillary action has been eliminated. A 50 µl cell, for example,
requires only additional 20 µl to completely fill the measuring
chamber. With the exception of the windows, these cells are made
entirely of black quartz.

The cells are constructed so that filling and emptying can easi-
ly be accomplished with commonly available pipette tips, as is
shown in the diagram at left.

Handling
• Filling: The large cylindrical bore of the cell and the funnel-
shaped reduction above the measuring chamber allow the sample
to be directly pipetted into the measuring chamber.
• Emptying: The sample may be removed completely by inserting a
pipette tip into the chamber as deeply as possible.

Ultra-micro Cells with PTFE stopper

Path External Aperture Center Volume Filling Part No.
Length Dimensions Height Volume Quartz
[mm] H x W x D [mm] H x W [mm] [mm] [µl] [µl]
2 45 x 12.5 x 12.5 2.5 x 2 15 10 20 5062-2497
10 45 x 12.5 x 12.5 2.5 x 2 15 50 70 5062-2496

12
Standard Cells

Ultra-micro Cells – with Eppendorf pipette

filling/emptying
These cells are designed to handle extremely small volumes.
Since often only a minute amount of a sample is available, these
cells had to be designed with a filling volume only slightly larger
than the measuring chamber volume.

Despite the extremely small measuring chamber volume, the

chamber aperture is large enough for proper positioning in the
light path.

Filling and emptying of the cells is accomplished through short

funnel-shaped openings, which end in the measuring chamber
channel directly behind the windows. To fill the cells, pipette tips
are inserted into the openings and 10 pi (or 5 µl) are injected into
the cell using a suitable pipette. Best results are obtained by
using tips for extremely small volumes, such as the ”crystal” tips
made by Eppendorf, where the conical shape of the tip exactly
matches the angle of the funnel-shaped openings. The use of
tips, which do not match the shape of the openings, can result in
sample loss or the appearance of air bubbles in the measuring
channel.

Handling
• Filling:
a) Draw the sample (5–10 µl) into the pipette tip and insert the tip
carefully but firmly into one of the conically shaped openings in
the top portion of the cell. Slowly release the sample into the
measuring chamber while holding the cell at an angle to allow air
bubbles to escape through the other opening.
b) Check that no air bubbles are present in the measuring chamber.
Bubbles present can be removed by tilting the cell and reactivating
the pipette.

• Emptying: The sample can be removed through one of the pi-

pette tips. We recommend to set the pipette to a larger volume
than the filling volume.

13
 Standard Cells

• Cleaning: The measuring chamber can be cleaned and air dried by

connecting pieces of tubing to each of the two pipette tips and
allowing cleaning solution, distilled water, and air to flow through
the cell.

Ultra-micro Cells with Eppendorf

pipette filling/emptying Path External Aperture Center Volume Filling Part No.
Length Dimensions Height Volume Quartz
[mm] H x W x D [mm] H x W [mm] [mm] [µl] [µl]
0.1 40 x 12.5 x 12.5 1x5 15 0.5 5 5063-6562
1 40 x 12.5 x 12.5 1x5 15 5 10 5063-6563
5 40 x 12.5 x 12.5 0.8 round 15 2.5 5 5063-6564
10 40 x 12.5 x 12.5 0.8 round 15 5 10 5063-6565

14
 Cylindrical Cells

General Description
A cylindrical cell is a cell with plane-parallel optical surfaces
whose inner volume is cylindrical in shape and has a
longitudinal axis parallel to the direction of the radiation beam.

The cells are equipped with two fittings to facilitate filling and
emptying of the cells. It is recommended not to remove the cylin-
drical cell between blank and sample measurement.

Cylindrical Cells with PTFE stopper

Path Length External Internal Volume Part No. Part No.
[mm] Dimensions Diameter [µl] Glass Quartz
L x Diameter [mm] [mm]
100 102.5 x 22 19 28000 5063-6566 5061-3392

15
 Flow-through Cells

General Description
Measuring Chamber: Flow-through cells, until now, had measu-
ring chambers that are either rectangular or circular in shape.
These shapes were dictated by limitations in the manufacturing
processes. Such cells do not offer the best geometry for clean
flushing and thereby reduced cross-contamination. We have
now introduced new oval apertured cells, which combine low
volume with excellent flow characteristics. These oval flow cells
are strongly recommended for automated analyses such as
dissolution testing.

Black quartz is used in the vicinity of the aperture to ensure that

no light passes through the side walls of the cell.

Volume: In the tables, where the flow-through cells are listed, we

have specified in the column ”volume” the measuring volume,
i.e. the volume of liquid, which is actually irradiated. Please note
that, depending on the cell type, the filling volume can be signifi-
cantly larger.

Light Beam Cross Section: It is important especially for cells with

very small apertures, to ensure that the light beam lies in the
center of measuring chamber.

Center Height: A very important dimension for flow-through cells

is height of the center of the light beam. All our flow cells have
the aperture centered at 15 mm from the bottom of the cuvette.

Bubble Formation: Flow-through cells are often subject to bubble

formation in the measuring chamber, which can lead to errone-
ous measurements. This problem is most prominent in cells with
light paths and small apertures. In the design of all our flow-th-
rough cells, one or all of the following steps are taken to combat
the problem:
• A dome-shaped cavity, into which bubbles can rise and thus
cannot interfere with the measurement, is introduced above
the measuring chamber.
• Each flow-through cell is subjected to a special chemical
treatment that inhibits the adhesion of bubbles to the surfaces
within the measuring chamber.

16
Flow-through Cells

• The channel that runs from the inlet tube to the outlet tube is
designed to have minimal changes in cross section.

We also recommend that you note the following points:

• The choice of suitable inflow and outflow tubing, such as the
recommended PTFE or FEP, is in part dependent on their cross
section.
• When working with a sipper system you should make sure that
the partial vacuum does not get too high in order to prevent
gases from coming out of solution.
• Each cell should be thoroughly cleaned and rinsed after it has been
in use for a given interval of time.

Carry-over: Carry-over occurs when, during the process of

changing samples residual fluids of one sample become mixed
with the next sample or the cleaning solution. This phenomenon
can lead to inaccurate results. Carry-over is influenced by the
following factors:
• design of the cell
• method used for filling / emptying the cell
• physical properties of the sample, such as viscosity and surface
tension.

Flow Rate: The flow rate is dependent on the cross-sectional area

of the inlet/outlet tubing, the smallest cross-sectional area within
the cell, the power of the pump being used, and the physical
properties of the sample (viscosity). Because of the many factors
influencing the flow rate, we are unable to offer any details for
specific cell types.

Pressure Load: Every single flow-through cell is pressure tested at

3 bars during final inspection. Due to their design, many cells can
withstand much higher pressures. However, the maximum sustai-
nable pressure is also dependent on the type of connectors used. For
example, compact cells with screw connectors con sustain higher
pressures than cells with glass inlet/outlet tubes, and are pressure
tested at 5 bars.

17
 Flow-through Cells

Cleaning: These cells should not be submerged in a cleaning

bath, but rather the cleaning solution should be pumped through
them. Connect the inlet tubing, via a pump, to a container holding
the cleaning solution. Both the inlet and outlet tubings should
lead into the cleaning fluid container.

As a cleaning solution for this closed circulatory system we

recommend water to which 2 % cell cleaning fluid
(5062-8529) has been added.

The cells should be rinsed with distilled water. Once the water
has passed through the cell it should be disposed of and not re-
circulated.

The cells may under no circumstances be put in a cleaning bath.

Such baths will destroy compact cells relatively quickly, espe-
cially if heat and/or ultrasound are used to increase the effec-
tiveness of the cleaning process.

Dirt on the outer surfaces of the cell can be removed with a

damp cloth. The above-mentioned cleaning solution or another
solvent such as alcohol can be used for this purpose.

Handling Instructions for Compact Cells with Screw Fittings:

A compact cell is a flow-through cell to which the tubings are
connected by means of screw fittings.

The cell consists of two parts, the actual quartz or glass cell
body and a U-shaped frame mode of black anodized aluminium.
The aluminium frame has taps for the fittings and is glued to the
cell body. The glue is only applied to the side surfaces of the cell.

Screw fittings into place finger tight, starting with the fitting with
the shorter screw connector. Do not use any tools such as pliers
to tighten the fittings.

18
 Flow-through Cells

Should a leak between the cell body and the flange occur, its
cause may be one of the following:
1. The screw connectors were not tightened adequately or have
loosened themselves. Check the tightness of the screw con-
nectors periodically, especially after long periods of use, and
tighten the connectors if necessary.
2. The flange at the end of the tubing is broken or damaged.
3. The holes on the top of the glass body of the cell are damaged
or chipped, thus preventing the flange from creating a proper
seal. For this reason, no hard or sharp tools should be used for
cleaning.
4. There are dirt particles present between the flange and the
top of the cell.
5. Component parts of the screw fitting were replaced with parts not
having the proper dimensions (such as a screw connector with too
short a thread).

Please check for any of the above causes before sending in a claim
for a leaking cell.

Flow-through Cells with round

aperture, screw fitting connections Path Length External Aperture Center Volume Part No.
[mm] Dimensions Diameter Height [µl] Quartz
H x W x D [mm] [mm] [mm]
10 35 x 12.5 x 12.5 2 15 30 0100-1224
10 35 x 12.5 x 12.5 3 15 80 0100-1225

Note:
Flow-through cells do not in-
clude tubing/fittings. See page
31 for tubing and fittings.

19
 Flow-through Cells

Flow-through Cells with rectangu-

lar aperture, Path Length External Aperture Center Volume Part No.
screw fitting connections [mm] Dimensions Diameter Height [µl] Quartz
H x W x D [mm] [mm] [mm]
1 35 x 12.5 x 12.5 17.5 x 3.5 15 62 5061-3396
2 35 x 12.5 x 12.5 17.5 x 3.5 15 124 5061-3397
5 35 x 12.5 x 12.5 11 x 3.5 15 230 5065-9918
10 35 x 12.5 x 12.5 11 x 3.5 15 390 5061-3398
10 35 x 12.5 x 12.5 8x2 15 160 5062-2476

Flow-through Cells with oval aper-

ture, screw fitting connections Path Length External Aperture Center Volume Part
No.
[mm] Dimensions Diameter Height [µl]
Quartz H x W x D [mm] [mm] [mm]
1 39 x 12.5 x 12.5 8x3 15 40
5063-6570
2 39 x 12.5 x 12.5 8x3 15 80
5063-6571
5 39 x 12.5 x 12.5 8x3 15 200
5063-6572
The new flow cell (PN 5065-9907) with an optimized shape of the
measuring chamber, inlet and outlet channels exhibits superior
flow characteristics. Compared with different shaped cells the
time to exchange the liquid in the cell has been minimized. This
allows the user to reduce pump time, sample volume and mini-
mize cross contamination. The new cell has been compared with
a similar cell (path length and volume) by using the flow test util-
ity of the Agilent UV-Visible ChemStation Software and the 8453
Spectrophotometer. With a flow rate of 6 ml/min 99.5% of the
volume in the new cell is exchanged within 14s, whereas it takes
more than 30 s to replace 99.5% of the volume in the other cell.
Flow characteristics
(1 mm path length)

20
Spacers
For cells with outer depth of less than 12.5 mm spacers are
required in order to hold the cell properly in the cell holder.
Description Part No.
Spacer for 1 mm cell 5061-3388
Spacer for 2 mm cell 5061-3389
Spacer for 5 mm cell 5061-3390

Stirring Bars
Stirring bars are for use with cells having internal dimensions of
10 x 10 mm (W x D) and cell holders that have magnetic stirring
capability.

Description Part No.

Magnetic stirring bar (2/pk) 9301-1161

Cell Tray
Description Part No.
Cell tray for 16 cells (10 mm) 5063-6577

Cleaning paper
Description Part No.
Lens cleaning paper, lint free (50/pk) 9300-0761

21
Cleaning Fluid
Description Part No.
Cell cleaning fluid, 1l 5062-8529

Cells serve as vessels for liquid or gaseous substances whose

interaction with light is to be observed, investigated, or measu-
red. To ensure that these photometric measurements are not
influenced or even become erroneous, great demands must
be placed on the cleanliness of cells. The cleaning of cells is
of great importance, as it demands the complete removal of all
contaminants without causing any damage to these delicate and
high-quality optical components.

The cell cleaning fluid is an alkaline liquid concentrate, which

must simply be mixed with water to yield an effective cleaning
solution for quartz and glass cells. It can also be used to clean
other sensitive optical components made of glass, quartz, sap-
phire, and porcelain.

The cell cleaning fluid completely removes even the most stub-
born contaminants such as fats, oils, waxes, dried blood, prote-
ins, silicon oil, and traces of many other organic and inorganic
substances.

Characteristics: The cell cleaning fluid significantly reduces the

surface tension of water. The removal of dirt particles is also assured
by the good wetting action of the aqueous solution, while the high
emulsifying and dispersing capabilities prevent the re-deposition of
the loosened particles. Special surface-active substances facilitate
the residue-free rinsing of the optical components once they have
been cleaned.

The fluid is low in phosphates and its cleaning agents are biode-
gradable. This product therefore complies with the newest require-
ments for the reduction of environmental pollution. Highly corrosive
and etching substances such as potassium hydroxide and chlorine
were specifically replaced with cleaning agents, which are gentle on
materials and skin.

22
As far as cleaning power is concerned, the cell cleaning fluid is
a complete replacement for chromosulphuric acid. Furthermore,
we specifically warn against the use of chromosulphuric acid on
cells, which contain materials other than quartz or glass (such as
plastics or metals).

The cell cleaning fluid is filtered to 1 µm and is therefore virtually

free of solid particles.

Cleaning: The optimal dilution depends on several factors, such

as the hardness of the water, the degree and type of contami-
nation, the temperature, etc. The use of de-mineralized water
improves the cleaning characteristics.

The cells are placed in a bath containing an acqeous solution of

cleaning fluid. Flow-through cells are cleaned by pumping the
cleaning solution through the cell in a closed cycle. The duration
of the cleaning cycle depends on the degree of contamination
and may last up to several hours. An increase in temperature
speeds up the cleaning process. However, since thermal shock
must be avoided, the cells must be pre-warmed before being
submerged into hot cleaning solutions. The following table list
conditions that have proved useful in practice.
Cleaning treatments which have proved useful in practice
Concentration [% by volume] Temperature [°C] Time [minutes]
0.5–2 25–30 120–180
0.5–2 30-35 30–40
0.5–2 50–60 (quartz only) 10–15
0.5–2 70–80 (quartz only) 2–5

Caution should be taken when using ultrasound to clean cells.

Although ultrasound improves the cleaning process noticeab-
ly, especially at higher temperatures, commercially available
ultrasonic baths do not lend themselves to the cleaning of cells.
Powers that are too high or cleaning durations that are too long
can result in cavitation damage, especially if the cell is placed
on the bottom of the cleaning bath. Compact cells and all other

23
 cells, which are made of several materials (glass, metal, plastic)
must never be placed in an ultrasonic cleaning bath.

Rinsing: After treating the rinse them carefully with demineralized

and filtered water. (Conductivity below 1 µS/cm) We recommend
Ultra Pure Water, part number: 5062-8578

Composition: Wetting agents, emulsifiers, ampholytic surface-ac-

tive agents, completing agents, and potassium phosphate.

Material Suitability: The cell cleaning fluid when diluted to its

working concentration, is completely suitable for use with glass,
quartz, sapphire, porcelains, ceramics, plastics, and ferrous me-
tals. It can also be used, with some limitations, on corrosion sen-
sitive metals such as aluminium and other non-ferrous metals.

The concentrate can be stored in vessels made of stainless

steel, polyethylene, polypropylene, polyvinylchloride, or similar

Physical Data
pH values: at 0.5 % (by volume): 10.7
at 1.0 % (by volume): 11.2
at 2.0 % (by volume): 11.5
Concentrate density: 1.42 g/cm3
Surface tension: at l.0 % (by volume): 30.10 mN/m
Phosphate content: at 1.0 % (by volume): 0.92 mg/cm3

24
8452A Spectrophotometer
Description Part No.
Deuterium lamp for HP 8452A and 8452A, option 002 08452-60104
Tungsten lamp for HP 8452A, NIR version 08452-60121

08452-60104

8453 Spectrophotometer
Description Part No.
Deuterium lamp 2140-0605
Tungsten lamp G1103-60001

2140-0605 G1103-60001

Literature
Description Publication No.
Fundamentals of modern UV-Visible Spectroscopy –
Primer 5965-5123E
Fundamentals of modern UV-Visible Spectroscopy –
Workbook 5965-6357E

Application Notes
Description Publication No.
Good Laboratory Practice with a UV-Visible
Spectroscopy System 5963-5615E
UV-visible spectroscopy as an alernative to liquid
chromatography a analysis method for dissolution testing 5968-8810E
Quantitative UV-Visible Analysis in the Presence
of Scattering 5963-3937E
Uses of Derivative Spectroscopy 5963-3940E
Measuring Very Low Volume Samples with a
Diode-Array Spectrophotometer 5964-3965E
Determination of Total Protein Contents
by UV-visible Spectroscopy 5968-1999E
Screening Environmental Samples Using
UV-Vis Spectroscopy 5988-1557EN

25
Cell holders
Description Part No.
Standard cell holder 08451-60104

A jacketed cell holder for standard 1 cm cuvettes.

Description Part No.

Thermostattable cell holder 89054A
08451-60104

A jacketed cell holder for standard 1 cm cuvettes with provisions

for attaching to a water bath (not supplied) with temperature-re-
gulated, circulating water.

Description Part No.

89054A Cell stirring module 89055A

Add to the 89054A thermostattable cell holder to provide stirring.

The stirrer is driven by the circulating water from the water bath
(not supplied).

Description Part No.

89076A
Long path-length cell holder 89076C

For cylindrical and rectangular cells with up to 10 cm path length.

Includes stops at 1.2 and 5 cm.

Multicell transport
Description Part No.
8-position multicell transport for 8453 G1120A

Includes water thermostattable 8-position cuvette holder for

standard 1 cm cuvettes with provisions for attaching to a water
bath (not supplied) with temperature-regulated
circulating water.
G1120A

26
Peltier Temperature Controller
Description Part No.
Peltier temperature controller 89090A

Includes digital controller with LED display of set and actual tem-
peratures and other parameters, choice of C, F, K units, magnetic
stirrer control and HP-IB interface.

Includes heat-exchanger for pre-heating samples for flow cells,

solid state magnetic stirrer. The temperature range is 10–100 °C.

Description Part No.

External sensor 89090A#200

For measuring the actual temperature in the sample. Includes

molded plastic collar for precise, air-tight fit in ”stoppered” cu-
vette or secure positioning in standard 1 cm rectangular cuvet-
tes. PTFE coated for inertness.

Autosampler
Description Part No.
XY Autosampler G1811A

XYZ robot with stationary rack design. Provides automatic

sampling of up to 240 samples; minimum sample volume is 2ml.
Includes wash station and set of 4 racks (code 21, 13x100mm).
Compatible with Gilson 20 or 30 series racks. Requires sipper
system below.

Sipper system
Description Part No.
Automated sipper system for
HP 8453 spectrophotometer 89068D

Includes peristaltic pump, flow cell (0100-1225, 10 mm path

length, 80 µl), tubings, fittings and interface cable.

27
Cell Holder Supplies
Description Part No.
Optical Filter Wheel, to prevent photosensitive samples
from being irradiated by UV light. Comprises three filters
(265 and 295nm cut-off, and UV roll-off filter). Can be mounted
on standard cell holder (08451-60104) and thermostattable cell
holder (89054A) 08451-60302

Cell stirring module for the thermostattable cell holder 89054A 89055A

Magnetic stirring bar (2/pk) for use with

08451-60302 cells of 10 x 10mm (W x D) 9301-1161

89075C
Description Part No.
7-Position Multicell Transport
Optical Filter Wheel, to prevent photosensitive samples from
Supplies
being irradiated by UV light. Comprises two filters with cut-off
wavelength at 265 and 295nm and one UV roll-off filter. 89075-60002

Multicell transport adjustment tool 89075-23800

89075-23800

G1120A
Description Part No.
8-Position Multicell Transport
Supplies Optical Filter kit. Set of three optical filters to prevent
photosensitive samples from being irradiated by UV light
(265 and 295nm cut-off, and UV roll-off filter). G1120-68707

Stirring module kit. The stirrer is driven by the circulating

water from the water bath (not supplied). G1120-60006

Magnetic stirring bar (2/pk) for use with cells

of 10 x 10mm (W x D) 9301-1161

Multicell transport adjustment tool 89075-23800

G1120-68707 Plastic cover kit G1120-68708

28
89090A
Description Part No.
Peltier Temperature Controller
Supplies Heat exchanger tubing, for pre-heating of samples 5042-1336
Union, cell holder (Kel-F) 5021-1870
Flow cell, 10 mm, 8 x 2 mm aperture, 160 ml 5062-2476
Quartz Cuvette, 10 mm, with PTFE stopper 5062-2477

G1811A
Description Part No.
XY Autosampler Supplies
Needle G1811-23200
Rack for 13 x 100mm tubes (9 ml) G1811-04500
RS 232 connector cable 5181-1520

Sipper Supplies
Description Part No.
Sipper tubing kit 5042-1333
Sipper/Autosampler tubing kit 5042-1334
Flow cells see pages 19–20
Cassette, fixed pressure 5041-2167
Cassette, variable pressure 5042-1356
Sipper/sampler cable for 8453 G1103-61609

5042-1356
5041-2167

29
Tubing and Fittings
Description Part No.

Teflon tubing 0.8 mm id., 1.6 mm od., 10 m 5041-2191

Pump tubings 2.06mm inner diameter (12/pk) 5041-2166

Pump tubings 1.3mm inner diameter (12/pk) 5041-2184

Pump tubings 2.8mm inner diameter (12/pk) 5041-2185

Tefzel Ferrules (10/pk) 5022-2154

Union (10/pk) 5022-2155

Cell fittings, black

(8/pk, 4 short and 4 long fittings) 5022-2156

Conical adapter kit,

connects teflon tubing 5022-2157
to pump tubing (2/pk)

Teflon Nuts, 1/16" (10/pk) 5022-2158

Peek fittings and ferrules

for 8-port valve (10/pk) 5042-1337

Spiral wrap, 2 m 5041-2200

Waste tubing, 80 cm 5042-1335

Mounting tool for

flange less nuts 0100-1710

8710-1930 Cutter for plastic tubing 8710-1930

30
Dissolution Testing System
Description Part No.
Supplies
Tubing/Fitting kits Multicell system tubing kit 5042-1330

Valve tubing kit for one bath 5042-1331

5042-1332
Dissolution probes kit 0.9mm id 5042-1332

Peek fittings and ferrules for 8-port valve (10/pk) 5042-1337

Valve, for 89079 Valve Unit 5063-6575

Dissolution filters for 1/8 in. probe, 45 µm pore size (1000/pk) 5181-1246

Flow cells see pages 19–20

5042-1330

5042-1331

31
Certified Calibration Standards
Note:
Certified calibration standards
are shipped with a Certificate
of Analysis. The Certificate of
Analysis for the batches can
be viewed on our web site at:
www.agilent.com/chem
32
In recent years quality requirements, as outlined by
ISO 9000, GLP, GMP and NAMAS have assumed increasing im-
portance and as a consequence, in the pharmaceutical industry,
the recommendations of pharmacopoeias have become more in-
fluential. Formal performance verification of UV-Visible spectro-
photometers is now essential but, because of the non-availability
of appropriate and/or easy-to-use standards, it has been done
either in ways which do not fully meet regulatory requirements or
it has been very time consuming.
It is important to note that GLP requirements specify that verifi-
cation of instrument performance should be done in such a way
that ”... the system or subsystem performs as intended throug-
hout representative or anticipated operating ranges”. The key
words here are as intended and representative operating ranges.
For a UV-Visible spectrophotometer used in the pharmaceutical
environment the consequences of this are, for example, that
the instrument should be verified for photometric accuracy
and wavelength accuracy in the UV range and not in the visible
range. Also, since the intended sample types are usually liquids
in cuvettes, the instrument performance should be verified using
liquid standards in cuvettes.
Agilent Technologies has made performance verification of the
8453 spectrophotometer much easier by making available sets of
liquid standards in snap-open ampoules.
These standards have the following advantages:
• They fully meet the recommendations of the US and European
Pharmacopoeias.
• They are ready-to-use. Just snap open the ampoules and use.
There is no preparation time and no chance of error.
• They come with a certificate of analysis and are traceable to
NIST standards.
• Because they are prepared in bulk they are inexpensive compared
to other types of standards.

The standards can be used with standard open-topped cuvettes but,

to reduce the time required and to minimize errors due to cross-con-
tamination from standard-to-standard, we recommend the use of flow
cells for the verification. A hardware kit is available which includes
all the necessary items.

The reusable hardware kit makes testing

reliable, quick and easy

33
Certified calibration standards
Note:
Ultra pure grade water
(5062-8578) is recommended
as blank for the stray light
tests.
34
Standards kit-I contains:
Standard
60.06 mg/l potassium dichromate in
0.01N sulphuric acid
0.01N sulphuric acid
12 g/l potassium chloride in water
10 g/l sodium iodide in water
50 g/l sodium nitrite in water
0.02% v/v toluene in hexane
hexane
* European Pharmacopoeia test methods
** ASTM methods
Standards kit-II contains
Standard
40 g/l holmium oxide in 10 % v/v
perchloric acid, traceable to NIST 2034 and visible ranges
10 % v/v perchloric acid
Purpose
Photometric accuracy
at 235, 257, 313 and 350 nm *
Blank for photometric accuracy test
Stray light at 200 nm *
Stray light at 220 nm **
Stray light at 340 nm **
Resolution (ratio of the absorbance
maximum at 269 nm to the absorbance
minimum at 266 nm*)
Blank for resolution test
Purpose
Wavelength accuracy in UV
Blank for wavelength accuracy
Standards and accessories for
system validation Description Part No.

OQ/PV Standards kit-I for UV-Vis 5063-6503

OQ/PV Standards kit-II for UV-Vis 5063-6521

OQ/PV Test sample for the Validation of Dissolution

Sampling systems (Cross Contamination Test).
150mg/l caffeine in water optimized for tests with
1 mm path length cells at 273 nm. 5042-6476

OQ/PV Manual G1115-90006

OQ/PV Hardware kit, contains 2 flow cells (10mm),

tubings and syringes, cell cleaning fluid, multicell
transport adjustment tool, temperature sensor
support, and OQ/PV manual 5063-6523

Tubing kit for UV-Vis OQ/PV test, contains:

2 sets of tubings, fittings and adaptor to
OQ/PV standards kit and hard- flush flow cells. 5063-6522
ware kit
Multicell transport adjustment tool 89075-23800

Hoya 056 filter, used for performance verification

of HP 8452A 08450-60300

Checkout Samples The following are checkout samples only (not certified)

Description
Holmium oxide glass filter Part No.
08450-601l7

IQ Test sample for UV-Vis (Caffein solution) 5063-6524

35
For greater confidence in your
UV-visible spectroscopic re-
sults, you can optimize your to-
tal system with quality supplies
from Agilent – the perfect fit
with your Agilent instrument.
A full line of cuvettes, supplies
and test standards are desig-
ned, manufactured and tested
to our rigorous specifications
under a quality system regis-
tered to ISO 9001. So why risk
compromising your analytical
results with anything less than
genuine Agilent consumables?

A perfect fit with your spectro-

photometer also reduces the
risk of system failures and loss
of productivity.

For more information on our products visit

our home page on the worldwide web at:
www.agilent.com/chem/chemsupplies

Copyright © 2002 Agilent Technologies

Printed in USA
April 15, 2005

Publication Number 5988-5967EN