1.

USE THE FOLLOWING OPTIONS TO ANSWER THE QUESTIONS THAT FOLLOW

A. CHONDROITIN SULPHATE

B. DERMATAN SULPHATE

C. HEPARAN SULPHATE

D. KERATAN SULPHATE

E. HEPARIN

f. HYALURONIC ACID

a) IDENTIFY THE GAG USED FOR CELL MIGRATION DURING EMBRYOGENESIS

b) IDENTIFY THE GAG USED TO IMPROVE TRANSPARENCY OF THE EYE

c) WHICH GAG LACKS URONIC ACID AS PART OF ITS CONSTITUENTS

d) IDENTIFY THE GAG ESSENTIAL FOR PROMOTING CELL- CELL COMMUNICATION DURING
DEVELOPMENT

e) IDENTIFY THE GAG WHICH AIDS IN GLOMERULAR FILTRATION WITHIN THE BASEMENT MEMBRANE

2. A 4-YEAR-OLD PRESENTS WITH CHONDROITIN SULPHATE IN URINE

a) CHONDROITIN SULPHATE CONTAINS

A. GalNAc AND GlcUA

B. GlcN AND GlcUA

C. GalNAc AND IdUA

D. GlcN, IdUA, GlcNAc AND GlcUA

b) CHONDROITIN SULPHATE BINDS TO CORE BY

A. GlcNAc-Asn

B. GalNAc- Thr

C. GalNAc-Ser

D. Xyl-Ser

E. NO ANSWER
c) WHICH OF THE FOLLOWING ENZYMES IS DEFICIENT IN THIS CHILD

A. β- GALACTOSIDASE

B. β- GLUCURONIDASE

C. ACETYL TRANSFERASE

D. N- ACETYL GLUCOSAMINE-6-SULPHATASE

E. GALACTOSAMINE-6-SULPHATASE

d) WHICH OF THE FOLLOWING MUCOPOLYSACCHARIDOSES WILL LEAD TO APPEARANCE OF

CHONDROITIN SULPHATE IN URINE

A. HURLER

B. SANFILLIPO

C. MORQUIO A

D. MORATEAUX-LAMY

3. WHICH TYPE OF ISOMER IS REPRESENTED BY EACH PAIR OF SUGARS

A. D-SORBOSE AND D-PSICOSE

B. D-GLUCOSE AND D-ALLOSE

C. D-FRUCTOSE AND L-FRUCTOSE

D. D-XYLULOSE AND D-RIBULOSE

E. α-D-GLUCOSE AND β-D-GLUCOSE

a) EPIMERS

b) ANOMERS

c) ENANTIOMERS

d) KETO PENTOSES

e) ALDO HEXOSES
4. THE SUBSTITUTION OF HYDROGEN FOR THE HYDROXYL GROUP AT CARBON 6 OF L-GALACTOSE
PRODUCES

A. L-FUCOSE

B. L-RHAMNOSE

C. L-RIBOSE

D. DEOXYRIBOSE

E. L-MANNOSE

5. THE POSITION OF THE ANOMERIC CARBON OF FRUCTOSE IS CARBON NUMBER

A. 1

B. 2

C. 3

D. 4

D. 5

6. SUCROSE CONSISTS OF

A. Glc(1α 2β) Fru

B. Gal(β1 4)Glc

C. Glc(β1 4)Glc

D. NONE OF THE ABOVE

7. REFER TO THE FOLLOWING FOR QUESTION 7-10

1. GlcNAc

2. GlcN

3. Gal

4. GalNAc

5. GlcUA
6. IdUA

A. 1 AND 5

B. 1 AND 6

C. 2 AND 5

D. 2 AND 6

E. 3 AND 5

F. 3 AND 6

G. 4 AND 6

H. 4 AND 5

7. HYALURONIC ACID

8. KERATAN SULPHATE

9. HEPARAN SULPHATE

10. DERMATAN SULPHATE

11.IN A HEALTH SUEVEY, FIVE STUDENTS V, W, X, Y AND Z HAD THEIR SERUM PROTEIN DETERMINED.
THE ABSORBANCE OF STANDARD PROTEIN(6g/dl), BLANK AND SERUM SAMPLES FROM V, W, X, Y AND
Z WERE 0.565, 0.005, 0.285, 0.585,0.345,0.215 AND 0.725 RESPECTIVELY. USE THIS INFORMATION TO
ANSWER THE QUESTIONS

a) THE NORMAL RANGE OF PROTEIN (g/dl) IN SERUM IS

A. 65-85

B. 6.5-8.5

C. 6.5-85

D. 5.6-5.8

b) THE PROTEIN CONCENTRATION FOR V AND Z RESPECTIVELY WERE

A. 3.0 AND 7.7

B. 2.3 AND 6.2

C. 3.6 AND 7.7

D. 2.3 AND 6.3

E. 6.2 AND 7.0

c) WHICH STUDENTS HAD NORMAL SERUM PROTEIN CONCENTRATION

A. V AND W

B. X AND Y

C. V AND Z

D. W AND Z

E. V, X AND Z

d) WHICH STUDENTS HAD ABNORMAL SERUM CONCENTRATION

A. V AND W

B. X AND Y

C. V AND Z

D. W AND Z

E. V, X AND Y

e) WHICH ONE OF THE FOLLOWING METHODS WAS USED IN THIS EXPERIMENT

A. DIACETYLMINIXIME

B. JAFFE’S METHOD

C. JENDRASSIC

D. BUIRET METHOD

E. BCG DYE BINDING METHOD

12. THE UNITS FOR MOLAR EXTINCTION COEFFICIENT ARE

A. L mole-1 cm-1

b. L mole cm-1
c. mole -1 cm-1

d. cm L-1 cm-1

13. WHAT IS THE ABSORBANCE OF A SOLUTION THAT TRANSMITS 1% INCIDENT LIGHT

A. 1

B. 2

C. 3

D. 4

E. 5

14. IN A TYPE 11 DIABETES SURVEY, FIVE STUDENTS V, W, X, Y, AND Z HAD THEIR PLASMA LEVELS
DETERMINED. THE ABSORBANCE OF STANDARD GLUCOSE (200mg/dL), BLANK AND PLASMA FROM V,
W, X, Y AND Z WERE O.565,0.005,0.285,0.585,0.345,0.215 AND 0.725 RESPECTIVELY

a) THE NORMAL RANGE OF GLUCOSE IN THE PLASMA SAMPLE IS

A. 6-14

B. 1.4-6

C. 60-140

D. 0.6-14

E. NONE OF THE ABOVE

b) THE GLUCOSE CONCENTRATION FOR V AND Z RESPECTIVELY WERE

A. 75 AND 121

B. 207 AND 100

C. 75 AND 100

D. 100 AND 257

E. NONE OF THE ABOVE

c) WHICH STUDENTS HAD NORMAL GLUCOSE CONCENTRATIONS

A. V AND W

B. X AND Y
C. V AND Z

D. W AND Z

E. V, X AND Y

15. WHICH CURVE REPRESENTS BEER-LAMBERTS LAW

ABSORBANCE

CONCENRATION

A. A
B. B
C. C
D. A AND C
E. NONE OF THE ABOVE

16.WHICH CURVE REPRESENTS THE RELATIONSHIP BETWEEN TRANSMITTANCE AND PATH LENGTH

% TRANSMITTANCE

PATHLENGTH

A. A
B. B
C. A AND B
D. NONE OF THE ABOVE

SHORT ANSWER QUESTIONS

17. HOW DOES %TRANSMITTANCE VARY WITH

a) INCREASING CONCENTRATION
b) INCREASING PATH LENGTH

18. THE ABSORBANCE OF AN IRON THIOCYANATE SOLUTION CONTAINING 0.00500 mg Fe/Ml was
reported as 0.4900 AT 540 nm

a) CALCULATE THE SPECIFIC ABSORPTIVITY, INCLUDING UNITS, OF IRON THYOCYANATE ON THE

ASSUMPTION THAT A 1cm CUVETTE WAS USED

b) WHAT WILL BE THE ABSORBANCE OF THE SOLUTION IF THE SOLUTION IS DILUTED TO TWICE ITS
ORIGINAL VOLUME

NEXT WEEK WILL BE ISOENZYMES