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Benedict’s Test Procedure

Definition:
Benedict’s test is a procedure performed to detect simple sugars such as glucose
Materials:
Benedict’s qualities reagent
Test tube
Alcohol lamp
Droppers (2pieces)
Urine (Early morning, mid stream flow)
Match
PROCEDURE RATIONALE
1. Place the paper lining clean side out on a table
or any clean, flat surface away from the client.
2. Spread the plastic lining over the paper lining,
clean side in.
3. Place the bag in the area away from the client,
tucking handles beneath the bag.
4. Open the bag; take out soap in dish, towel and
apron.
5. Do thorough hand washing. Dry hands, place
soap/dish and towel near the bag.
6. Put on apron, right side out.
7. Take out the kidney basin and place 3 CB with
alcohol.
8. Take out 2 droppers, a test tube and holder,
and place these in the kidney basin. Take out the
alcohol lamp, match .Benedict’s sol’n and place
them on the work area.
9. Place the waste receptacle at one corner of
the work area.
10. Light the lamp.
11. Place 6-8 drops of urine in the test tube.
12. Add 3-5 ml. Of Benedict’s sol’n to the urine

Used droppers should be placed near the waste


receptacle.
13. Pass the test tube with mixture over the
flame, mouth of the TT should be away from you
and the client.
14. Remove from flame and stand for one
minute. Put off flame.
15. Interpret:

Clean blue to marked cloudygreen———


negative

Bluish green—————————————-+

Yellowish green————————————++

Yellow————————————————-+++

Orange————————————————++++
16. Wash the TT, TT holder and droppers with
soap and water. Drain,place them in the work
area.
17. Disinfect the kidney basin, TT, TT holder and
droppers with 3 pieces of CR with alcohol,
Discard 2CB. Place the articles inside the kidney
basin(Save the last CB with alcohol)
18. With one CB with alcohol, disinfect the lamp
and the plastic lining.
19. Discard the waste receptacle.
20. Do thorough hand washing. Dry Hands with
the towel.
21. Return all articles in their proper places in
the bag
22. Remove apron away from the body, folding
contaminated part in. Place over the flaps of the
bag.
23. Fold the plastic lining contaminated part in.
Place over the flaps of the bag.
24. Hold the bag with one hand, and with the
other hand fold the paper lining , contaminated
part in. Place over the plastic lining.

Close the bag.


Student’s Grade:
C.I. Signature:

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