Sputum Collection AFB

INDICATION:
1.To detect mycobacterial infection.
2.To diagnose TB (mycobacterium TB)
3.Monitor effectiveness of treatment.
When is the best time to collect the sputum?
The best time, but not the only time, to collect a sputum specimen is upon awakening in
the morning.
PRECAUTION:
Precautions to keep the specimen out ofsunlight at all times will be taken because
ultraviolet light kills the TB bacteria and exposure to sunlight could alter the test result.
After specimen collection the container should be shielded from sunlight during transport
into the facility.
PREPARATION:
1.Bottle for sputum collection
2. Blue colour form
3.Gloves
*Label innermost tube with patient name and date of birth before obtaining specimens
and before giving container to patient.

Procedure Rationale
1.Wash hand
2.Greet client and explain procedure
3.Provide privacy.
4. Identify client by checking ID band
5. Give bottle for sputum collection.
6. Specimen collection should be done
without the client rinsing the mouth or
brushing teeth.

7. The client should be instructed to sit or

stand and open the specimen container

8. Inhale deeply 2-3 times, breathe out hard

each time.

9. Cough deeply from the chest

10. Place the open container close to the

mouth to collect the specimen.

11. Trace lab results ( AFB within 24hours)

12. Analyse the result