ENZYME

Enzyme Mechanism

Enzymes do two important things:

• they recognize very specific substrates,
• they perform specific chemical reactions
on them at fantastic speeds.
 ACTIVE SITE
The active site is a specialized region of the
protein where the enzyme interacts with the
substrate

TRANSITION STATE
The transition state is the highest-energy
arrangement of atoms during a chemical
reaction.
 CATALYSIS
The reaction happens at a faster rate. The
catalyst is regenerted

LOCK AND KEY

Specificity model—the correct substrate fits into
the active site of the enzyme like a key into a
lock. Only the right ke fits
 INDUCED FIT

The binding of the correct substrate

triggers a change in the structure of the
enzyme that brings catalytic groups into
exactly the right position to facilitate the
reaction
 NONPRODUCTIVE BINDING

Poor substrates bind to the enzyme in a large

number of different ways, only one of which is
correct. Good substrates bind only in the proper
way.
ENZYME KINETICS
• The concentrations of substrate and product
are invariably in molar units (M; this includes
mM, M, etc.), but
• enzyme concentrations may be given in molar
(M), milligrams per milliliter (mg/mL), or
units/mL.
• The amount of enzyme you have can be
expressed in molecules, milligrams, nanomoles
(nmol), or units. A unit of enzyme is the amount
of enzyme that will catalyze the formation of 1
mol of product per minute under specifically
defined conditions.
• A unit is an amount, not a concentration.
Line Weaver Burk
=
+

1 1 1
= +
Eadie Hofstee
=
+1

+ =

=− +
Hanes-Wolf
1 1 1
= +

= +
COMPETITIVE INHIBITION
 .
=
+ = E.S
= + +
Kecepatan reaksi: =
.
=
1+ +
1 1 1
= + 1+

′ = 1+
UNCOMPETITIVE INHIBITION
NONCOMPETITIVE INHIBITION
Pengaruh pH pada Aktivitas Enzim
Pengaruh Suhu pada Aktivitas Enzim