1. Dengan menggunakan program BLAST (http://blast.ncbi.nlm.nih.gov/Blast.

cgi)
a. identifikasi masing-masing urutan nukleotida yang diberikan
Sequence 5: Eschericia coli

b. tentukan apakah kedua urutan tersebut mengkode protein, jika yam aka cari
informasi terkait protein tersebut

Query length: 666

2. Dari sekuens yang diberikan , carilah 10 sekuens DNA serupa yang berasal dari
beberapa spesies atau galur berbeda dan lakukan multiple alignment
pada tingkat DNA:
pada tingkat protein:
3. Carilah 20 sekuens organisme prokariot/eukariot dan buat pohon filogenetik.
Pohon filogenetik:
 4. Dari sequence DNA/Protein yang diberikan, carilah apakah terdapat urutan promotor,
inisiasi transkripsi, Open Reading Frame dan Terminator
Promotor:
Start End Score Promoter Sequence

91 136 0.87 gtattacgaaattgtcccaacacatgtgccagccgataaa aaccgaccgc

1118 1163 0.85 ttaatgccgcaagctgacgtaagcattctccggcatgata gtggctggcg
1708 1753 0.91 ttttccgtgggctggacggtggcacaaagtcgttgataac ttaacaccag

1 tccttgttgc tgaagcatgg ggaataattg ttcgcaaagt aaaacaccgt tattcattgc

61 ttctacccgt gcctcgcttt ctgtattacg aaattgtccc aacacatgtg ccagccgata
121 aaaaccgacc gcggagaggt cattcgccag caactctgcc tgactaatag cgctctgttc
181 ctgatagcgc cagccgttat ggagcagttg aataagtaac gcctggcagc gcatcagcaa
241 ctgatgagcg gtagacggca caggcaaaac gctggcagaa ggtagcggtg ccacaggcgc
301 agtttctgcg tccagcgccc aggcgcgggt ttttgtcatc atcacccgtg gttccagtgt
361 caattgccca tcaacaaaac tgacaaagcc agaaaccaga ctcacggggt cgtctgtttg
421 ttgcaaaagc gccgccatgc gttcaacggc aaaaggagaa caggcagatg ccggaaggga
481 taacgtcagg agattatctt caccttcgcc gctgattacc tgcgcatcca gcgtctggcg
541 gctgctatcc caaccgagcg aaatacactc agcgaccggc agaataaata agttatcgac
601 ctgattaaga ggccgtatgc aggcgggggg acgctggcgt aaatattccc gcaaagccac
661 aatgcccggc tggcgtaacg gcgcgctcaa catttgccag gcatcaggcg acagcggcac
721 aacgctgctt aagcggttgc gggtagctaa cagcagctcg ccatcggcac tgcgttttgc
781 tgcttgtgaa acaatttgcc cacccgccag tgcgccagcc tgaaaactaa acagccgacg
841 cgtagctgcc ggtgagtttt cctgttcact tcgcggccaa ctgcgcgaaa ggtgcaaaat
901 actgccggtg tcgggatcgg taaaccagat gcgtaaacca taatgctcaa tatcctgcca
961 gcaacgcata cctaaagaca ccagccgcag atgatcaagc tttgcttctc cggcaatgcc
1021 agagccaacg accgtgcgcc acggcacagg aggaacttca ccaacactgt cgcgccgggc
1081 catctcttgt gcgcaattta atcgactgtt taatgccgca agctgacgta agcattctcc
 1141 ggcatgatag tggctggcgc gagtgtggaa ggcatcaacg cttgcccgta gctgtcgtag
1201 tgattcactc acccagcgcc agttgcaggc ctctgccgcc tgcaatgcgc ggttgaatgc
1261 tgcctcgtag tgaataagcg gctggctgat gccgccaagc cataatgcct ggcttaattg
1321 ctgaacatat tgacgacacg tttggccttc ttcgctggca aacggatcgt cagatgatgt
1381 gacgtgttcg ctgcgcatct gccagattaa atggttaaat tctgcttgct gcgctttggc
1441 ctcgacgaag gcctgcacag ccagtacgac atgttcgcaa agtgtgcctt caatacaatc
1501 acaacgggcg aaacgaatac tgctacggga ataaaaacgc acatcgctca tcggtaagcg
1561 ggcagaggga atttcgcccg gcgcacagaa caactcaatg gtgatgcctt tagcgaccag
1621 cgcctgcgcg cgtttgcggg tggcatcggg cagggtagcc agttcttcca gccagattgc
1681 cggatcccac tcttcttctt tttccgtggg ctggacggtg gcacaaagtc gttgataact
1741 taacaccagc atcacgcgat gacggcacat gccgctggcc ccgcagctgc actgagcctc
1801 tttcagtgcc tggctgttcg ccagctgggt acggacaccg tcactgaagg tggcgattaa
1861 agcgctgttc tcatggctga tttccgggac gttgccattt tccagttcct taagactgcg
1921 cttaacaaaa ccggcattgc ttaacgccgt cagggcctgc ggtgtcagtt ctaataattc
1981 cggacgtagt gaattcat

tccttgttgctgaagcatggggaataattgttcgcaaagtaaaacaccgttattcattgcgcctcgctttctgta
ttacgaaattgtcccaacacatgtgccagccgataaaaaccgaccgcggagaggtcattcgccagcaactctgc
ctgactaatagcgctctgttcctgatagcgccagccgttatggagcagttgaataagtaacgcctggcagcgcat
cagcaactgatgagcggtagacggcacaggcaaaacgctggcagaaggtagcggtgccacaggcgcagtttctgc
gtccagcgcccaggcgcgggtttttgtcatcatcacccgtggttccagtgtcaattgcccatcaacaaaactgac
aaagccagaaaccagactcacggggtcgtctgtttgttgcaaaagcgccgccatgcgttcaacggcaaaaggaga
acaggcagatgccggaagggataacgtcaggagattatcttcaccttcgccgctgattacctgcgcatccagcgt
ctggcggctgctatcccaaccgagcgaaatacactcagcgaccggcagaataaataagttatcgacctgattaag
aggccgtatgcaggcggggggacgctggcgtaaatattcccgcaaagccacaatgcccggctggcgtaacggcgc
gctcaacatttgccaggcatcaggcgacagcggcacaacgctgcttaagcggttgcgggtagctaacagcagctc
gccatcggcactgcgttttgctgcttgtgaaacaatttgcccacccgccagtgcgccagcctgaaaactaaacag
ccgacgcgtagctgccggtgagttttcctgttcacttcgcggccaactgcgcgaaaggtgcaaaatactgccggt
gtcgggatcggtaaaccagatgcgtaaaccataatgctcaatatcctgccagcaacgcatacctaaagacaccag
ccgcagatgatcaagctttgcttctccggcaatgccagagccaacgaccgtgcgccacggcacaggaggaacttc
accaacactgtcgcgccgggccatctcttgtgcgcaatttaatcgactgtttaatgccgcaagctgacgtaagca
g
ttctccggcatgata tggctggcgcgagtgtggaaggcatcaacgcttgcccgtactgtcgtagtgattcact
cacccagcgccagttgcaggcctctgccgcctgcaatgcgcggttgaatgctgcctcgtagtgaataagcggctg
gctgatgccgccaagccataatgcctggcttaattgctgaacatattgacgacacgtttggccttcttcgctggc
aaacggatcgtcagatgatgtgacgtgttcgctgcgcatctgccagattaaatggttaaattctgcttgctgcgc
tttggcctcgacgaaggcctgcacagccagtacgacatgttcgcaaagtgtgccttcaatacaatcacaacgggc
gaaacgaatactgctacgggaataaaaacgcacatcgctcatcggtaagcgggcagagggaatttcgcccggcgc
acagaacaactcaatggtgatgcctttagcgaccagcgcctgcgcgcgtttgcgggtggcatcgggcagggtagc
cagttcttccagccagattgccggatcccactcttcttctttttccgtgggctggacggtggcacaaagtcgttg

ataac ttaacaccagcatcacgcgatgacggcacatgccgctggccccgcagctgcactgagcctctttcagtg
cctggctgttcgccagctgggtacggacaccgtcactgaaggtggcgattaaagcgctgttctcatggctgattt
ccgggacgttgccattttccagttccttaagactgcgcttaacaaaaccggcattgcttaacgccgtcagggcct
gcggtgtcagttctaataattccggacgtagtgaattcat

ORF 1: 106-209

ORF10: 1113-1256

ORF4: 1759-1860
TERMINATOR
>CP055256.1:1821442-1823439 Escherichia coli strain AH25 chromosome, comple
te genome
    294  Rnamotif + CAGGCGCAGTTTCTGCGTCCAGCGCCCAGGCGCGGGTTTTTGTCATCA -9.50
    417  Rnamotif - TGTTCTCCTTTTGCCGTTGAACGCATGGCGGCGcTTTTGCAACAAA -7.80
   1919  Rnamotif - GCAGGCCCTGACGGCGTTAAGCAATGCCGgTTTTGTTAAGCG -9.10

Total number of predicted transcription terminators: 3