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18.00

So in this video, we're going to talk through the shore scream covid-19 antigen test, this test
comprises of a cassette and the spot that and into a buffer to running the sample to the test and
we're taking something from each nostril, is there is interior Mary's test so it's really easy to to
conduct and it will give you the result in 10 minutes.

So first thing to do, when testing is to get the cassette and check on the back to make sure that the
test is within its expiry date. Then it's a case of opening the pouch here and getting cast out ready to
be used, put that down here.

And then we need to do the collection. And so we're going to use the swamp and samples going to
be taking from from my nose. And then we'll be sample through the food, the cassette. So, when it
comes to collection, what we want, is the The Collector to be wearing PPE gear, so that there's some
protection. Therefore, from any sort of antigen positive person. Also it A good idea to make sure that
you're fully aware of the information in the public insert as well. So I'll introduce Allison. She'll come
on and take it, take a sample. So good luck Commander.

So Alice is going to use the interior noise swab to swamp boat of my nostrils and the first thing I'm
going to do is actually blow my nose. So that moved any sample from the back of the nose, to the
front of the nose, make sure we get a good, a good sample on the Swap. This what needs to go in?
Probably a couple of centimeters and then it's rotated again. It's the front wall of the names up here
in this area, just below the bridge of the nose.

I'm so thirsty decided to give the razor blade. Okay, great. So if you like to come and do this,

Okay, so if you just hang on to that for a moment. So what we need to do now is to just prepare the,
the buffer solution. So we have a file here, which we use to mix the buffer and the swap together.
So, what we need to do is Twist off the top of the little vials here, like so.

About him to be vile. Yeah.

Great. So if you want to pop the swab in there and then what we want to do is to pinch this improve
it. There's a flexible part of the little vial here. And by turning that what we're doing is transferring
sample into the book that which will help sort of break it down, so it can flow through your testing,
test can see if there's any antigens present. So, we do that around 10 times and then What we need
to do is actually break this swab. There's a little sort of Notch halfway down and we do that so that
the bottom of the buffer remains in the in the Bible like so there is a case of closing the top over
which works as a dropper, and then we just add the buffer into the cassette. So it requires three
drops So I'll add the sample now. So it's best to add one. And then let it absorb before, adding the
next one. So I've dropped to, and the last drop So, now the buffer is going to flow through the test
and you'll probably see a pink wash coming up through the results window. And then we need to
resolve me the result and compare it to the information and the instruction booklet. So we visit 10
minutes. So 10 minutes have elapsed and it's time to have a look at the resort on the cassette. So
they're actually really easy to to read and what we're looking for is a line at the control region of the
top deck by C and then the the presence or the absence of test line at T. So in the case of a negative
result, like this one, there's no line that forms at the T position. So, it seems that a negative result
with a positive. We'd expect to see a firm line fatigue as well. So as you can see you know it's using a
test to administer just getting a sample from the front of the nose. It's less invasive than within like a
nasopharyngeal test. For example, if you'd like to know anymore about this test these contactors
which your screen, thanks

Throw flowers at technology.

Lover of flowers. Our technology is a very, very straightforward, very effective. Technology for Rapid
tests, the rapid Diagnostics are kind of what it sounds like. You're getting your diagnostic answer
between to 15 minutes. Unlike the traditional testing methods where there's long incubations, it'll
be hours before you get a lab result. Rapid Diagnostics means you have your sample and within You
know, waiting at the doctor's office. You have an answer. So that's where the flow is. Where we take
reagents, like, antibodies are binders that detect certain analytes, we put them onto specific
materials, like, nitrocellulose and different types of pads with pads. And then we can encapsulate
that into a cassette of plastic housing and that holds the device and protects it. Then we combine
that with potentially a sample hand. Labeling methods for instance, taking a finger stick blood
applying that to the test and running the test in very quickly. I'm getting a result on the spot so I
think the example that comes to mind when you talk about lateral flow is the pregnancy test. That is
lateral flow in its most recognizable form. But that same platform where you see one or two limes to
indicate a yes or no.

Be applied to a lot of different things. And not just to tell you, whether you're pregnant or not, it
could be used to say, whether you have an infectious disease like malaria or HIV or covid-19. So, let's
get started with making our lateral flow test. So, step one, we want to prepare in solution tubes of
our control line, antibody and our test line. Antibody. That's why you see two lines. So the Test line,
antibody is the antibody that recognizes the thing you're testing for. So for pregnancy, it's HCG. It
can be different for different applications. Once you have them prepared in the solutions, you can
then dispense it onto nitrocellulose that we have here and this is how you get the two lines.

So, I'm going to place my membrane, make sure it's flush to the bottom. So that my positioning of
the lines is the same from membrane to membrane. And here, you can designate the x b, y and the Z
of these needles right here. First, I want to select the dispense pattern so I'll select front lines since
I'm stripping the membrane and I'm going to go through each parameter and make adjustments
accordingly and then I hit go. So there's two parts Lateral flow. There's we're dispensing, the capture
antibody, and then later, we will dispense the detector antibody. So I've just finished up marking
where the lines were dispensed. I've labeled the control line, where we dispensed, our control
antibody to ensure that the test was run correctly. And then, here are test line, that's kind of the line
that we care. The most about that is where our capture antibody. Body is, and then you would let
that dry in the oven for 30 minutes. And then the second major aspect of your lateral flow strip is
the conjugate. So what is the conjugate? The conjugate is your other antibody that would detect
your antigen and form a sandwich. But on that, antibody will have a visual particle so that we can
see it. When we strive on the test line, we can't really see the antibody other than, you know,
initially when you dispense, so So, in order to visualize whether to antibodies bound to an antigen,
we need one of the antibodies to be labeled to a visual particle. And this visual particle will dry down
and dispensed into what we call a conjugate pad. And then later, we will take all of these and we will
put them together to form our lateral whole strip. Alright so now we are going to dispense our
detection label and the detection label we aren't going to strike like we did for the capture. We are
going to spray it so it's a little bit more spread out. So I'm going to disable our Frontline since we're
no longer dispensing. Onto a membrane and I will enable the air Jets. And now we are good to go
when we strive where immobilizing it. But here we're only kind of temporarily storing. It this allows
us to keep Regent dry down onto a material and allows lateral flow to be run in various different
conditions and places traditional laboratory tests, you would need to use a pipette in order to use
this region but with

18.14

Sprayed, with our detector and a body. And one more part of the lateral flow strip, is the wick pad or
the absorbent pad. This will allow the liquid to flow from the bottom to the top in a continuous
fashion. So what I'll need is is backing card. This is what is going to hold all of the different
components of my lateral flow strip together. So first, I'll take out the adhesive liner. And I will take
our membrane where we dispense the capture antibody and line it on the card.

And then just to make sure it adheres. Very gently press.

Then our absorbent pad on top. Flush to the top. So, we are still making contact with the membrane.
This contact is what allows the liquid to Wick through.

And in this trip, we are just going to have a conjugate pad.

And then just like we did for the wick we are making sure that there is some contact from the
conjugate pad to the membrane and then press it in place. And then now I have an assembled card
ready to cut into strips Now that we have our assembled strip now it's time to cut them to our final
strip so we're just going to load the card here and then adjust our with and how many strips will kind
of the time.
And now we have our final strip ready to put into a casa. So, now that we've cut our card, we have
our lateral, flow, strip, and we are almost done. So, at this point, we will place it into the cassette, or
some sort of device. And at this point is kind of where things start looking like finished products out
there. And we'll have a sample port, where we'll put our liquid. And then we have a window where
we

We'll see either one line or two lines and you can close it either Mallet or through this cassette
roller.

And you have your assembled device ready to run. Alright. So now we have our final letter flow
because that and I am going to test to see if we get positive or negative. So I prepared a positive
solution and we should see two lines. Once it is done, developing in 10 minutes,

Now at this stage we are ready to take this test to either other labs for it to be deployed there or to
be deployed out in the field and your doctor's office or even at home. That's the beauty of lateral
flow.

But this on a screen and drag for one hour and we're now ready to assemble our test.

18.19

So now it's time to put everything together. We have our dried dispensary agents, we have our
nitrocellulose membrane with are immobilized capture antibody. We have our pandu pads, braid
with our detector, and a body. And one more part of the lateral flow strip, is the wick pad or the
absorbent pad. This will allow the liquid to flow from the bottom to the top and a continuous
fashion. So what I'll need is this backing card, this is what is going to hold all of the different
components of my lateral flow strip together. So first I'll take out the adhesive liner and I will take
our membrane where we dispense the capture antibody and line it on the card.

And then just to make sure it adheres very gently press.

Then our absorbent pad on top. Flush to the top. So, we are still making contact with the membrane.
This contact is what allows the liquid to Wick through.

And in this strip, we are just going to have a conjugate pad.

And then just like we did for the wick we are making sure that there is some contact from the
conjugate pad to the membrane and then press it in place. And then now I have an assembled card
ready to cut in distress. Now that we have our assembled strip now it's time to cut them to our final
strip so we're just going to load the card here and then adjust our with and how many strips will kind
of the time.