INSTRUCTION MANUAL HVG-1 HYDRIDE VAPOR GENERATOR (P/N 206-17143) SHIMADZU CORPORATION SPECTROPHOTOMETRIC INSTRUMENTS PLANT ANALYTICAL INSTRUMENTS DIVISION KYOTO, vAPAN 122-£5764TABLE OF CONTENTS 1, Parts Checking ....... 2. Installation ..... 2.1. Installation Table and Layout 2.2. Installation Requirements os... 2.3. Required Facility ss... 2.3.1 Power Supply 2.3.2 Carrier Gas... 2.4 Assembling 24.1 Line Voltage Setting 2.4.2. AC Cord Connection 2.4.3 Gas Hose Connection 2.4.4 Absorption Cell Mounting 2.4.5 Pump Tube Connection ssesssssene Gas/Liquid Separator Mounting Carrier Gas Tube Connection... Drain Tube Connection sj. Front Cover and Side Cover Mounting... Guide Plate Installation 3. Construction and Operational Principle ......cesstssesseserses 3.1 Names and Functions of Control Parts 3.1.1 Front Panel... 3.1.2. Side Panel 3.1.3 Rear Panel 3.2 Principle of Operation 3.3 Construction 3.4 Specifications 4, Operation 4.1 Preparation Bunn e e eewD oe i it 12 13 14 15 16 17 74.1.1 Reagent Preparation 7 4.1.2 Sample Preparation... 18 4.1.3 Reagent Loading . TE 18 4.1.4 Pump Preparation 18 4.1.5 Speed Controller Setting 19 4.1.6 Atomic Absorption Spectrophotometer Start-up susetaene 19 4.2. Measuring Operation ses 20 4.2.1 General Measurement Procedures 20 4.2.2 Combination with AA-660/670/680 snes » 4 4.2.3. Flush Switch Operation 21 4.3. Shutdown Operation ssseuetintusestneuetitnsienavasenennense 22 5. Connection with ASC-60F _.. 23 6. Maintenance 24 6.1, Pump Tube Replacement 24 6.2 Absorption Cell Maintenance .. 24 | 6.3. Gas/Liquid Separator Maintenance ssisssstsnsneniststintestnnneetnees 25 Te Repair Parts nssunnninnnnnnnn 26 7.1 Consumables ssssavanenansvensaneones 26 7.2 Replacement Parts. sacssssssstnesetntnsistssietnenetntneneetee 261. PARTS CHECKING When uncrated, make sure that the following parts are included. Table 1. A list of standard components No. Description Quantity] PartNo. 1 | Hydride vapor generator main body 1 2 | Reagent bottle (polyethylene) 2 | 204.09363-01 3. | Gashose 1 | 204.05845.02 4 | Drain tube (Tygon®) 1 | 206.60250-02 5 | AC cable (for 100 ~ 117V area) or 1 | 20613774 | Power cable (for 220 ~ 240V area) 200-81008-02 6 | Fluorinated rubber tube 1 | 206-60250-01 7 | Guide plate 1 | 20618033, 8 | Fuse 0.5A (for 100 ~ 117V line) or 2 | 07201023 Fuse 0.3A (for 220 ~ 240V line) 072.01660.06 9 | Cell holder 1 | 206-18034 10 | Absorption cell 2 | 20066584-03 11 | Hose band 1} 037-61019 12. | Pump tube 080 (for reagent) 4 | 200-54612-08 13 | Pump tube 130 (for sample) 2 | 200-54612.13 14 | Front cover 1 | 206.17878 15 | Side cover 1 | 206.17879 16 | Gas/liquid separator 1 | 206.17839 17 | Manifold 1 | 206.17825 18 | Resection coil 1 | 206-18037 19 | Aspiration tube (yellow-marked) for NaBH 1 | 206-18036-01 20 | Aspiration tube (blue-marked) for HCL 1 | 206.18036-02 21 | Aspiration tube (not marked, 30cm) for sample 1 | 206-18036-03 22 | Aspiration tube (not marked, 80cm) for ASC 1 | 206-18036-04 23 | Wire mark (yellow) 5 |072-83083.03 24 | Wire mark (blue) 5 |072-83083-02 25 | Vinyl cover 1} 206-18068 26 | Grounding line 1 | 20232982 27 | Label (flow path diagram) 1 |206-18616 28 | Instruction manual 1 | mi22.576| | \ | \ 2 ® Fig. 1 Standard components -2-2. INSTALLATION 2.1. Installation Table and Layout The present device is to be installed in front of the Atomic Absorption Spectrophotometer. Provide a table of the same height as that carrying the latter and place as illustrated in Fig. 2. Run the AC cord and the gas hose beneath the Atomic Absorption spectrophotometer. Gas hose |—AC cord About 200mm 220mm, OO 340 mm Fig. 2. Layout2.2 Installation Requirements ‘The same conditions as those for the installation site of the Atomic Absorption Spectrophotometer are required. It is necessary to keep the exhaust duct working during the ‘measurement s0 as to remove poisonous metal hydride gas emitted from the device. 2.3. Required Facility For operating the present device, the following facilities are required: 2.3.1 Power Supply Voltage: 100/115/117/220/240V Tolerable voltage range: within £10% Line capacity: 30VA Line frequency: 50/60H2 a 2.3.2 Carrier Gas Gas species: ‘Argon gas | Supply pressure: 3.2.4 0.2 kgflem? Consumption: 70ml/min Purity: 99.9% or better2.4 Assembling 2.4.1 Line Voltage Setting Pull out the line voltage selector at the rear of the present device, insert a fuse as specified in Table 2 and remount the selector so that a numeric value corresponding to the line voltage to be used is displayed (Fig. 9). Table 2 Line voltage and fuse rating Tine Volage | Woltage Selector Scring | Fuse T00V area 100 [ose 115V area | 17 0A 117 area | 220V area 220 | 0.34 240V area 240 [oan 2.4.2. AC Cord Connection Make sure that the POWER switch is OFF and connect the AC cord to a socket at the rear of the main body (Fig. 9). A power outlet with grounding is to be used. Otherwise, the system is to be grounded from the grounding terminal. 2.4.3 Gas Hose Connection Connect the open end of the gas hose to the outlet of the gas cylinder and fasten it with a hose band. Then, connect the other end of the hose to GAS IN at rear of the main body (Fig. 9). 2.4.4 Absorption Cell Mounting (1) Place the cell holder on the standard burner head of the slot length 10cm and fasten with a screw belonging to the cell holder. (2) Connect one end of the fluorinated rubber tube to the inlet pipe of the absorption cell, and run the other end through the central hole of the cell holder. (3) While pushing both ends of the absorption cell to the leaf spring in the V-groove of the cell holder, insert the tip of the absorption cell entrance into the cell holder hole. Make sure that both ends of the absorption cell are not lifted above the V-groove of the cell holder. NOTES: . 1. When handling the absorption cell, wear clean gloves so as not to touch the cell with bare hands. 2. As the T-junction of the absorption cell is fragile and vulnerable, insert the cell to the tube while holding the pipe at the cell entrance. 5.Vigroove Leaf spring Absorption cell Fluorinated Screw rubber tube Fig. 3 _ Installation of the absorption cell 2.4.5 Pump Tube Connection (1) Fit two reagent pump tubes (provided with orange bridge) with yellow and blue wire marks so as to identify them as tube for NaBH, and that for HCl, respectively. (2) Pass the markless end of the NaBH, pump tube to the side panel through the left bottom hole of the front panel. (3) Connect the pump tube to the NaBH, port of the manifold, in reference to Fig. 4. ‘ (4) Connect the HCl pump tube in the similar way to the HCl port of the manifold. (5) Similarly, pass the sample pump tube (provided with blue bridge) from the front panel to the side panel, and connect it to the sample port of the manifold, (6) Connect the reaction coil at the outlet port of the manifold, and set the reaction coil and the manifold on the side panel as shown in Fig. 8. (7) Hook the bridges of NaBH, , HCl and sample pump tubes to the lower grooves of the tube holder, run tubes along the pump rotor, and hook bridges on the other side to the upper groove of the tube holder, Pump tubes are to be arranged from the recess to the front in this order: NaBHg, HCI and sample (Fig. 5).(8) Pass the NaBH pump tube to the side panel through the top left hole of the front panel, and connect the aspiration tube (marked yellow), (9) Similarly, connect the HCI pump tube with the aspiration tube (marked blue). (10) Hold the NaBH, and HCl aspiration tubes with the clamp at the side panel as shown in Fig. 8. (11) Hold the sample pump tube with the clamp at the side panel and connect with aspiration tube (not marked). NOTE: Insert the pump tube to the joint as deep as possible, Pull the tube lightly with a hand, so as to confirm that the tube does not come off, Reaction coil _—Port for outlet Port for sample Cartier gas inlet Port for NaBH Port for HCL 4 Diagram of manifoldYellow wire ‘mark Blue wire mark For NaBHs For HC For sample Brid, Bric Tube holder Rotor eR orange) rated bie) Fig. Diagram of pump tubes 2.4.6 Gas/Liquid Separator Mounting (1) Place the gas/liquid separator at the side panel and fasten with a rubber ring, as shown in Fig. 8. (2) Connect an end of the reaction coil to the reaction product inlet of the separator. (3) Connect to the gas outlet of the separator the free end of the fluorinated rubber tube connected to the absorption cell. (4) After having connected tubes, make sure that both ends of the absorption cell are not lifted from the V-groove of the cell holder. NOTE: Do not pull the fluorinated rubber tube strongly, otherwise the absorption cell may be displaced or damaged.New! Carrier gas inlet To absorption cell — Gas outlet Carrier gas tube Reaction product inlet j= From reaction coil 1 To drain reservoir Drain outlet Fig. 6 Diagram of the gas/liquid separator 2.4.7 Carrier Gas Tube Connection (1) Connect the shorter tube protruding from the top right of the side panel to the carrier gas inlet of the manifold. (2) Connect the longer tube protruding from the top right of the side panel to the carrier gas inlet of the separator. 2.4.8 Drain Tube Connection (1) Connect one end of the drain tube to the drain outlet of the gas/liquid separator. (2) Set an appropriate reservoir to receive drain and put the other end of the tube into i. The tube is to be suspended as straightly as possible so as not to let drain accumulate in the mid-portion of the tube. The tube is to be cut at an appropriate length so that the tip does not sink in the drain.2.4.9 Front Cover and Side Cover Mounting Insert the hinge pin of the front cover into the hinge of the front panel from the above. Slide the side cover along the guide pin of the reagent tray and hook the claw within the cover to the reaction coil holder. 2.4.10 Guide Plate Installation Align the tip of the guide plate footing to the pin hole of the reagent tray and set the guide plate, which prevent the reagent bottles from turning down, -10-3. CONSTRUCTION AND OPERATIONAL PRINCIPLE 3.1 Names and Functions of Control Parts 34d Cramp Joint Sample aspiration tube ——_ Z ee © 6oO®8 Front Panel | Guide plate POWER switch Flush switch Reagent tray Pressure monitor lamp Flush pilot lamp Speed control knob Pump tube Tube Screw fitting stopper Hinge | (For mounting ] | the front cover) To start up the device. When pressed, the rotor speed is accelerated for about 20 sec to flush the flow path with quick flow, Lights up when the supply gas pressure lowers below 2.8 kef/cm?. While lit, the rotor does not turn. To indicate the flushing action. To set aspiration rate of sample or reagent. Identified by color code. Bridge Color | Wire Mark Color NaBH | orange yellow HCL orange blue Sample | __blue one Adjuster screw To adjust the squeeze pressure on the pump tube. Fig.7 Front panel -1-3.1.2 Side Panel Joint Clamp Drain tube Carrier gas Carrier gas tube (longer) tube (shorter) (connected to Gaslliquid (connected to maniforld) separator) @ __ Reaction coil ‘To generate hydride through the reaction between sample and reagent while passing within, . @® _ Gas/liquid separator _To separate hydride from the residue after the reaction. ® Manifold ‘To mix NaBH, and HCI solutions and lead the mixture to the reaction coil. Fig.8 Side panel | a23.1.3. Rear Panel Voltage selector i 0.5A(100~117V) 0,84(200~240V) GND Carrier gas inlet Power connector Fig.9 Rear Panel -13- 1 Grounding terminal3.2. Principle of Operation Some elements in the aqueous solution, such as As, Se, Sn, Sb, Te or Bi, react with nascent hydrogen to generate gaseous hydride. Feeding hydride into the atomizer and pyrolyzing it to atomize allow to ensure the high sensitivity analysis. On the other hand, inorganic mercury (Hg) in the aqueous solution is vaporized as free mercury in the presence of reducing agent. Leading mercury vapor to the atomizer, the atomic absorption measurement can be made without heating. Sample, HCl and NaBH, solutions are fed into the manifold by the pump, mixed and sent to the reaction coil to generate hydride. ‘The reaction mixture composed of hydride, hydrogen, water vapor and reagent residues are fed to the gas/liquid separator by carrier gas. In the separator, the gas phase is separated from the liquid and sent to the absorption cell by carrier gas, while the latter is drained, The absorption cell is heated by the air-acetylene flame to pyrolyze hydride. ‘This allows to atomize the target element for the atomic absorption analysis. For the analysis of mercury, the same cell is operated without being heated. -14-3.3. Construc A flow path diagram is shown in Fig. 10. Absorption cell Burner head Peristaltic pump Manifold Reaction coil sanple ——+| (p< Hce)| ———» Gas/liquid separator Nabe os Nv PRK a MV Nva 1 Carrier gas 4Vt iT Drain Pressure switch | Solenoid valve Pressure regulator Needle valve Fig. 10 Flow path diagram -15-3.4. Specifications The specifications for the HVG-1 are given in Table 3. Table 3. Specifications Measurement mode | continuous flow method Reducing agent sodium borohydride (NaBHa ) Sample aspiration | 0 ~ 8 ml/min, variable Reagent aspiration | 0~ 3 ml/min, variable Atomizer heated absorption cell (heated by the air-acetylene flame) Carrier pas argon gas, supply pressure 3.2 kgf/cm*, consumption 70 ml/min. Power requirements | 100/115/117/220/240V AC, 50/60 Hz, 30 VA. Dimensions 340(W) X 220(D) X 200(H) mm 0 -16-4. OPERATION 4.1. Preparation 4.1.1 Reagent Preparation (1) Sodium Borohydride Solution (0.4% NaBH, solution) Dissolve 2.5gr sodium hydroxide and 2.0gr sodium borohydride in distilled water in this order and add water to 500m. NOTE: Aged NaBH, solution has the sensitivity reduced. The reagent is to be prepared immediately before use. 2) Hydrochloric Acid (for elements other than Sn: 5M HCl, for Sn: 0.5M HCl) ~~ Dilute HCI solution to make 500ml of 5M HCl. NOTE: With HCI solution of higher concentration, Sn will be interfered resulting in reduced sensitivity. 0.5M HCI solution is to be used. (3) Standard Solutions Prepare standards solutions of appropriate concentration. Some of standard concentrations are given below. Table 4 Recommended concentration ranges of standard solutions Elements | Concentration (unit: ppb) ) As 5~20 Se 10~40 Hg 20~ 80 Sn 30~90 Sb 5~20 Te 5~20 Bi 5~20 NOTE: Since the concentration of the standard solution is very low, elements in the solution may readily adsorbed to the container or evaporated. The standard solution is to be prepared immediately before the measurement. -17-4.1.2. Sample Preparation (1) Samples for As, Se and Hg are required to be inorganic. Organic samples are to be digested and ashed by an appropriate acid. (2) The measurement with As may give different sensitivity depending upon whether the oxidation state is [Il or V. It is necessary, therefore, to reduce the sample previously to AsIII. To get the AslIl sample, for example, add 2me of 20% W/V potassium iodide solution to 20m of the standard solution or the sample solution, and leave it for about 15 minutes. NOTES: 1. Since potassium iodide interferes with Hg, Te and Bi intensively, the analysis for these elements can not be made in the flow path having run KI. It is ‘ recommended to provide another set of tubes, manifold, gas/liquid separator and absorption cell and use in exchange. 2. Incase of Sn, it should be avoided to use HC] for the pretreatment. 4.1.3. Reagent Loading Install the bottles for 0.4% NaBH, solution and 5M HCI solution (or 0.5M HCI solution) with the latter in front. 444 Pump Preperation | (1) Loosen the adjuster screw fully, turn the screw stopper and open the tube fitting (2) Hook the pump tube bridge to the tube holder groove and set as shown in Fig. 5. | Tubes are to be arranged from the recess in this order: NaBH, , HCl and sample. NOTE: Set the tubes vertically to the roller without being twisted. | (3) Close the tube fitting, and turn the screw stopper so that the tubes are held lightly. (4) Make sure that the carrier gas is supplied at the pressure 3.2 + 0.2 kg/cm?, and turn the POWER switch ON. (5) Pour distilled water into a suitable container and put 3 aspiration tubes into it. (6) Turn the adjuster screw on the sample side little by little until the solution begins to be aspirated smoothly through the sample aspiration tube and further tighten by about 1/2 turn, (7) Similarly, tighten the adjuster screw on the reagent side until the solution begins to be aspirated smoothly through two reagent aspiration cube and further tighten by about 1/2 turn. ‘ -18-NOTES 1. Do not overtighten the adjuster screw. Otherwise, the service life of the may be reduced remarkably. 2. Itis necessary to run in the pump for about 10 minutes until the pumping | rate is stabilized. 3. When a new tube is used, the aspiration may fail even when the adjuster screw is fully tightened. In such a case, do not tighten the screw forcibly. Run in the pump for about 10 minutes with the screw tightened halfway and then set the screw again, 4.1.5 Speed Controller Setting ‘The sensitivity may be affected by the aspiration rate of sample and reagents, The sample aspiration rate is to be set normally to 5 ~ 6 ml/min. Generally speaking, the higher the aspiration rate, the higher the sensitivity, but the higher the noise level grows to deteriorate the reproducibility. It is recommended to set the aspiration rate to a previously determined value ensuring both appropriate sensitivity and good reproducibility. 4.1.6 Atomic Absorption Spectrophotometer Start-up (1) Set the lamp current, wavelength, slit and other parameters as specified in the Instruction Manual for the Atomic Absorption Spectrophotometer. (2) Adjust the absorption cell position so that the absorbance reading is minimized when the beam passes through the center of the absorption cell by the proper adjustment of the burner position. (3) Ignite the air-acetylene flame. For the analysis of Hg, no flame is needed. NOTES: 1. The acetylene flow rate is to be set to 2.0 lit/min, whatever element is being analyzed. The higher acetylene flow rate may result in the deposition of soot in the absorption cell, markedly reducing the service life of the cell. 2. When the atomic absorption spectrophotometer provided with the automatic gas stop mechanism involving a flame monitor is used, it may occur that the light emission from the flame is blocked by the absorption cell and the flame monitor is actuated to extinguish the flame automatically, In such a case, the flame monitor is to be turned OFF, -19-4.2. Measuring Operation 4.2.1 General Measurement Procedures (1) When the pump run-in is finished and the flame is ignited, put the reagent aspiration tubes into the specified bottles. Do not mis:place aspiration tubes identified by color marking: yellow-NaBH, and blue HCl. Make sure that the tube tip reaches the bottom of the bottle. (2) Set the sample and put the sample aspiration tube in. (3) After a short delay following the start of the sample aspiration, the absorbance signal increases and attains to a steady state in 30 ~ 70 seconds. (4) Make sure that the absorbance signal is stabilized and read signals. NOTES: 1, The time interval between the start of aspirating sample to the steady state may vary depending upon the element and the sample concentration. That for ‘As and Se is shortest with about 30 seconds, and Hg longest with about 70 seconds. 2. Do not extinguish the flame until the sample is fully expelled from the flow path so as to ensure the complete decomposition of poisonous hydride gas. 4.2.2. Combination with AA-660/670/680 (1) Set INSTRUMENT CONDITION to conditions for the target element. (2) Set MEASUREMENT CONDITION as shown below: SIGNAL PROC,: INTEG HOLD (INTEG or INTEG AV in case of the AA-660) INTEG TIME: 10 sec (3) Wait for about 5 minutes after ignitting and reset MODE. t Depress [STARTISTOP] key, and wait until the zero adjustment is finished. ) ) Set the sample and put the sample aspiration tube into the sample container. ) (6) When the absorbance signal reading is stabilized, press the following keys: For unknown sample: [MEASURE] key For blank solution: BLANK] key For standard sample: [STANDARD] key When the measurement is finished, the absorbance of that sample is printed out. (7) Repeat steps (4) through (5) with another sample. The following sample sequence is recommended: blank — unknown (or standard) sample — blank -- unknown (or standard) sample ~ blank -—- unknown (or standard) sample — blank ..... It is essential to alternate blanks and samples. -20-NOTES 1, The time interval between the start of sample aspiration to the steady state can be set as PRE-SPRAY TIME. In such a case, press [MEASURE] ( [BLANK] STANDARD] ) keys immediately after starting the aspiration. 2, In case of doing the measurement with As, Se, Sn, Sb or Bi by using the AA.660 in the BGC-SR mode, it should be noted that the sensitivity is reduced to 1/2 ~ 1/3 as high as that in the HCI mode. 4.2.3 Flush Switch Operation It may occur in the measurement with blank solution following that with sample of higher concentration, that it takes longer time for the signal to return to the zero level as shown in. Fig, 11, or the noise level increases. Such a phenomenon occurs frequently with Sb and also with Te and Bi. To avoid this trouble, put the aspiration tube into the blank solution and depress the flush switch as the signal begins to decline, The pump rotor starts to turn quickly to flush the flow path in a short time. When flushing is over and the zero level signal is stabilized, press [BLANK] key. Not flushing Absorbance signal Time Flushing Absorbance signal Time HIGH Pump rotor rate ° Low FLUSH switch depressed Fig. 11 Operation of the Flush switch -21-4.3 Shutdown Operation (1) When the measurement with the last sample is finished, put three aspiration tubes (sample and reagents) into distilled water, run the pump for 5 ~ 10 minutes to continue the aspiration 50 as to wash the flow paths. (2) Take aspiration tubes out of distilled water, and aspirate air through until the flow paths are freed from water droplet. (3) Extinguish the flame of the atomic absorption spectro photometer, and turn the POWER switch of the HVG-1 OFF. (4) Turn OFF the gas supplies. (5) Loosen the adjuster screw, turn the screw stopper and open the tube fitting. ) Release the pump tube from the rotor. ') Disconnect tubes from the absorption cell and the gas/liquid separator. Dismount these components, clean them with distilled water and dry in a clean place. NOTES: 1. The burner head, the cell holder and the absorption cell are heated to a very high temperature. The absorption cell is to be handled after having been cooled adequately. 2. After having finished the measurement, discard drain in the drain reservoir. Remaining sample is to be discarded separately. If it is joined with drain, the reaction with reagents in the drain may generate poisonous metal hydride, =22-5. CONNECTION WITH ASC-6OF When the HVG-1 is connected with the ASC-60F, the automatic analysis is available. Operate through the following procedures: (1) Replace the sample aspiration tube of the HVG-1 with that for the ASC connection. (2) Mount the ASC connecting aspiration tube to the tube holder on the sampling head of the ASC-60F. (3) In order to set the length of the aspiration tube as short as possible, install the ASC turn table as close as possible to the HVG-1. Cut the aspiration tube to a suitable Iength so that the tube is not forcibly pulled by the movement of the sampling head. (4) Determine the time interval from the start of the sample aspiration to the steady state of the absorbance signal, and set it as ASPIRATION TIME. When the HVG-1 is connected with the ‘AA-660/670/680, that time interval is to be set as PRE-SPRAY TIME. In the latter case, the setting for ASPIRATION TIME is ignored, and the sum of PRE-SPRAY TIME with INTEG TIME is newly set to ASPIRATION TIME. (5) Set ASPIRATION MODE to SOLVENT/SAMPLE. (6) For the other operation, follow the instruction given in the Instruction Manual for the ASC-60F. -23-6. MAINTENANCE 6.1 Pump Tube Replacement When the pump tube is aged, replace it through the following procedures: (1 (2) Loosen the adjuster screw, turn the screw stopper and open the tube fitting. Disengage the pump tube bridge from the tube holder. ic (5) ) ) (3) Take the manifold out of the side panel, and disconnect the aged tube from the joint. ) Disconnect aged tubes from the joints connected to three aspiration tubes. ) Connect new tube following the instruction given in 2.4.5, NOTES: 1, As the pump tube is aged, the aspiration rate declines slowly with time. It is 9 recommended to monitor the aspiration rate by using a measuring cylinder at times, and when the aspiration rate drops to about 80% of the initial level, replace the tube with new one. | 2. Do not leave the pump tube as squeezed under the rotor. This may reduce the service life of the tube markedly. 3, It is recommended to coat the pump tube with a small amount of silicone grease, to extend its service life. 6.2 Absorption Cell Maintenance ‘The absorption cell made of transparent quartz glass may be abruptly deteriorated if handled improperly. ‘The cell should be handled carefully with the following points taken into considera- tion. The standard service life is about 50 hours. 2 NOTES: 1. Clean the cell thoroughly with distilled water every day after finishing the operation. 2. Do not touch the cell with bare hands. Do not use the cell as contaminated. with dusts. Impurities such as sodium, potassium may accelerate devitrification and reduce the service life. 3. Do not clean the cell with surface-active agent or soap. Avoid to contaminate the cell with these agents. -24-The contamination of the absorption cell is unavoidable even when it is cleaned every day. If contaminated heavily, the surface of the absorption cell may be activated to reduce the sensitivity markedly. In such a case, soak the cell in about 25% nitric acid solution for 30 minutes or longer and rinse with distilled water thoroughly, 6.3. Gas/Liquid Separator Maintenance The separator is to be cleaned thoroughly with distilled water. As in case of the absorption cell, heavy contamination may result in reduced sensitivity and elevated noises. In such a case, soak the separator in about 25% nitric acid solution for 30 minutes and clean thoroughly with distilled water. -25-7. REPAIR PARTS 7.1 Consumables Parts listed below are consumables. Be careful not to use up spare parts. Whenever spare parts are used, give order quickly. Table 5 Consumables No. Description Part No. 1 | Pump tube 080 (for reagent) 200-54612-08 Pump tube 130 (for sample) 200-54612-13 3 | Absorption cell 200-66584.03 7.2. Replacement Parts The following parts are to be replaced when heavy contamination or damage should occur. Table 6 Replacement parts No. Description PartNo. 1 | Gasfliquid separator 206-17839 2 | Manifold 206-17825 3. | Reaction coil 206-18037 4 | Aspiration tube (yellow-marked), for NaBH, | 206-18036.01 5. | Aspiration tube (blue-marked), for HC] 206-18036-02 6 | Aspiration tube (not marked, 30em), for sample | 206-18036-03, 7 | Aspiration tube (not marked, 100em), for ASC | 206-18036-04 8 | Fluorinated rubber tube 206.60250-01 9 | Joine (for reagent) 206-17826-01 10 | Joins (for sample) 206-17826-02 -26-