FSSAI Report

ANALYSIS REPORT No. 2008120105 DATE: 12.08.
2020 PAGE 1/1
Client:
22008120105
PA300872
Dabur India Ltd.
9, N.S.C. Bose Road, P.O.: Narendrapur E-Mail: Intertek Indien
700103 Kolkata
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India
Our reference no. : PI2008110011

Product : Honey
Sample description / Batch : Ifs-200804021 - Bulk Honey - Batch No.: NP5190 -
: Mfg date. 30.07.2020
Sample received on / transported by : 10.08.2020 via Parcel service Seal : none
Sample temp. when received / stored : RT Sampling : Client
Packaging / Quantity : Plastic container / ca. 150g Start / End of analysis : 11.08.2020 / 12.08.2020
ANALYSIS REQUESTED: Macrolides-1 by LC-MS/MS (101050)

Parameter Result Unit Method
Tylosin A n.d. µg/kg PM DE01.059 (a) 1
Sum Erythromycin A n.d. µg/kg PM DE01.059 (a) 1
Lincomycin n.d. µg/kg PM DE01.059 (a) 1
n.d. - not detected < limit of quantification of 5 µg/kg

(a) : accredited method. (na) : not accredited method. (1) Inhouse procedure
This document may only be reproduced in full. The results given herein apply to the submitted sample only.
Interpretation:
Regarding the examined parameters and the mentioned limit of quantification the sample corresponds
to the legal regulations (Regulation (EC) 470/2009 in conjunction with regulation (EU) 37/2010 (dated Feb. 9th
2010)).
The result for tylosin A includes the identified metabolite tylosin B.
The result for sum erythromycin A includes the identified metabolite anhydroerythromycin A.
Hauke Zinow
Responsible Scientist, Certified Food Chemist
Intertek Food Services GmbH Tel.: +49 421 65 727 1 Sitz Bremen Geschäftsführer Durch die DAkkS nach DIN EN ISO/IEC
17025 akkreditiertes Prüflaboratorium.
Olof-Palme-Straße 8 Fax: +49 421 65 727 222 Registergericht Bremen, HRB 28046 Reinhold Gehling Die Akkreditierung gilt für die in der
28719 Bremen, Germany food.germany@intertek.com USt-IdNr. DE 185128973 Urkunde aufgeführten Prüfverfahren.
ANALYSIS REPORT No. 2008140642 DATE: 14.08.2020 PAGE 1/2
Client:
22008140642
PA300872
Dabur India Ltd.
700103 Kolkata
India

Product : Honey
: Mfg date. 30.07.2020
ANALYSIS REQUESTED: 13C Isotope Analysis by EA/LC-IRMS (C4/C3-Sugars) (101158)

Protein -25.58 d13C(%o) PM DE01.026:2019-05 (a) 1
Honey -26.20 d13C(%o) PM DE01.026:2019-05 (a) 1
Fructose (F) -26.28 d13C(%o) PM DE01.094:2019-05 (a) 2
Glucose (G) -26.01 d13C(%o) PM DE01.094:2019-05 (a) 2
Disaccharides -27.01 d13C(%o) PM DE01.094:2019-05 (a) 2
Trisaccharides n.a. d13C(%o) PM DE01.094:2019-05 (a) 2
Oligosaccharides n.a. d13C(%o) PM DE01.094:2019-05 (a) 2
Delta d13C (F-G) -0.27 d13C(%o) PM DE01.094:2019-05 (a) 2
Delta d13C (max.) 1.43 d13C(%o) PM DE01.094:2019-05 (a) 2
C4 Sugar Content 0.00 % PM DE01.026:2019-05 (a) 1
F/G Ratio 1.15 PM DE01.094:2019-05 (a) 2
Portion of Disaccharides 5.06 % PM DE01.094:2019-05 (a) 3
Portion of Trisaccharides n.d. % PM DE01.094:2019-05 (a) 3
Portion of Oligosaccharides n.d. % PM DE01.094:2019-05 (a) 3
LC-IRMS is not an official method for F/G ratio; n.a.: not analyzed; n.d.: not detected < 1.0 %;
d13C value in permil. vs. V-PDB standard
SPECIFICATIONS OF PURE HONEY:

Delta d13C (F - G): not more than +/- 1.0 %o
Delta d13C (max.) of individual d13C values: not more than +/- 2.1 %o
C4 sugar content: <= 7 %; portion of oligosaccharides: n.d.
(a) : accredited method. (na) : not accredited method. (1) AOAC 998.12:2013 (2) Apidologie 39/5 (2008) 574-587
(3) Apidologie 39/5 (2008) 574-587; % related to total sugars.
to be continued...
22008140642
PA300872
Interpretation:
Foreign sugars were not detected. Regarding the examined parameters, the sample meets the requirements for
unadulterated honey (Council Directive 2001/110/EC of 20 Dec 2001).
Ulrike Burmester
Dept. Head Authenticity, Chemical Engineer
Client:
22008120543
PA300872
Dabur India Ltd.
700103 Kolkata
India

Product : Honey
: Mfg date. 30.07.2020
ANALYSIS REQUESTED: Diastase activity with p-nitrophenol substrate (101206)

Diastase 7.0 DZ PM DE01.090:2014-04 (a) 1
n.d. - not detected < limit of quantification (LOQ) 0.1 DZ

(a) : accredited method. (na) : not accredited method. (1) Inhouse procedure (5E-pNP-G7)
Interpretation:
Regarding the examined parameter the sample corresponds to the legal requirement for honey if it is a naturally
enzyme-poor honey (like acacia and citrus honey) and its HMF content is not higher than 15 mg/kg (Art. 1 in
connection with Annex II Council directive 2001/110/EC).
Christopher Schielmann
Client:
22008130522
PA300872
Dabur India Ltd.
700103 Kolkata
India

Product : Honey
: Mfg date. 30.07.2020
ANALYSIS REQUESTED: Proline by Photometry (101211)

Proline 206 mg/kg DIN 10754, mod. (a) 1
n.d. - not detected < 50 mg/kg

(a) : accredited method. (na) : not accredited method. (1) 2002-08
Interpretation:
The examined proline content of the analysed sample lies within the expected range.
Thus, regarding the examined proline content, the analysed sample corresponds to the legal regulation
(EU honey directive 2001/110/EU dated Dec 20th, 2001, Article 2 No.1 in connection with Annex I No. 1, in
connection with relevant literature: Deutscher Imkerbund e.v, Honiguntersuchung, Prof. Dr. Jost H. Dustmann;
Reife, Naturbelassenheit und Unverfälschtheit von Honig, Dr. Werner von der Ohe, Niedersächsisches Landesinstitut
für Bienenkunde, Celle,1999).
Christopher Schielmann
Client:
22008140276
PA300872
Dabur India Ltd.
700103 Kolkata
India

Product : Honey
: Mfg date. 30.07.2020
ANALYSIS REQUESTED: Elements by ICP-MS (101491)

Arsenic (As) 0.019 mg/kg DIN EN 15763 mod. (a) 1
Lead (Pb) 0.025 mg/kg DIN EN 15763 mod. (a) 1
Cadmium (Cd) n.d. mg/kg DIN EN 15763 mod. (a) 1
Copper (Cu) 0.131 mg/kg DIN EN 15763 mod. (a) 1
Mercury (Hg) n.d. mg/kg DIN EN 15763 mod. (a) 1
Tin (Sn) 0.746 mg/kg DIN EN 15763 mod. (a) 1
n.d. - not detectable < limit of quantification (LOQ): Ag, As, Cd, Co, Cr, Cu, Hg, Mn, Mo, Ni, Pb, Sb, Se, Sn, Te: 0.01 mg/kg;
Li: 0.02 mg/kg; Ba, Fe, Mg, Tl, V, Zn: 0.10 mg/kg; Al, Ca, K, Na, P: 1.0 mg/kg
(a) : accredited method. (na) : not accredited method. (1) 2010-04
Interpretation:
Regarding the examined lead content and the mentioned limit of quantification the sample corresponds to the legal
regulations (Regulation (EC) 1881/2006. For all further examined elements there are no maximum levels in honey set
by the Regulation (EC) 1881/2006. The determined contents do not exceed the maximum levels set for comparable
foodstuffs and lie within the range of the naturally occurring range for honey (ref.: Swiss Food Compendium, Chapter
23A, EDMZ, 1995; Stefan Bogdanov, Contaminants of bee products, Apidologie 37 (2006); MAFF UK - Analysis of
bee products for heavy metals, MAFF Food Surveillance Information Sheet no. 53, Feb 1995).
Dr. Martin Schubert

Client:
22008120070
PA300872
Dabur India Ltd.
700103 Kolkata
India

Product : Honey
: Mfg date. 30.07.2020
ANALYSIS REQUESTED: Screening for specific marker - rice syrup by LC-MS/MS (SM-R) (101604)
SM-R (rice syrup) negative PM DE01_190 (a) 1
(a) : accredited method. (na) : not accredited method. (1) Inhouse procedure
Interpretation:
The result does not indicate an unauthorized addition of rice syrup in the investigated honey sample.
Hauke Zinow
Client:
22008130490
PA300872
Dabur India Ltd.
700103 Kolkata
India

Product : Honey
: Mfg date. 30.07.2020
ANALYSIS REQUESTED: Determination of honey-foreign oligosaccharides by LC-ELSD (101606)

Foreign oligosaccharides 0.12 % PM DE01.191:2019-05 (a)
n.a.: not analyzed; n.d.: not detectable < limit of quantification (LOQ), LOQ: 0.1 %;
Reported value represents foreign oligosaccharides with a degree of polymerization > 5
SPECIFICATIONS OF PURE HONEY: foreign oligosaccharides: n.d.
(a) : accredited method. (na) : not accredited method.

Interpretation:
The content of honey foreign oligosaccharides in this sample is equal or over the limit of quantitation (LOQ), but they
were detected in an amount that lies within the expanded measurement uncertainty of the LOQ and the positive
finding is therefore uncertain. Further analyses for adulteration with starch based syrups (e.g., 13C EA-/LC-IRMS,
activity of beta-/gamma-amylases, NMR-Profiling) are recommended.
Ulrike Burmester
Dept. Head Authenticity, Chemical Engineer

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ANALYSIS REPORT No. 2008120105 DATE: 12.08.

2020 PAGE 1/1

Our reference no. : PI2008110011

ANALYSIS REQUESTED: Macrolides-1 by LC-MS/MS (101050)

n.d. - not detected < limit of quantification of 5 µg/kg

Our reference no. : PI2008110011

ANALYSIS REQUESTED: 13C Isotope Analysis by EA/LC-IRMS (C4/C3-Sugars) (101158)

SPECIFICATIONS OF PURE HONEY:

Our reference no. : PI2008110011

ANALYSIS REQUESTED: Diastase activity with p-nitrophenol substrate (101206)

n.d. - not detected < limit of quantification (LOQ) 0.1 DZ

Our reference no. : PI2008110011

ANALYSIS REQUESTED: Proline by Photometry (101211)

n.d. - not detected < 50 mg/kg

Our reference no. : PI2008110011

ANALYSIS REQUESTED: Elements by ICP-MS (101491)

Dr. Martin Schubert

Our reference no. : PI2008110011

Our reference no. : PI2008110011

ANALYSIS REQUESTED: Determination of honey-foreign oligosaccharides by LC-ELSD (101606)

SPECIFICATIONS OF PURE HONEY: foreign oligosaccharides: n.d.

(a) : accredited method. (na) : not accredited method.

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