Tanggal :

RT PCR
Operator :
Alat : BIORAD
Kit : GB

½ Reaction Full Reaction

PCR Mix 1X 35X 35X

RT-Master Mix 12.5 µL 218.7 µL 437.5 µL

GB SR (100) 0.5 µL 8.75 µL 17.5 µL

Multiplex Mix 2 µL 35 µL 70 µL

15 µL

RNA/NFW/PC 10 µL

PCR Conditions Fluorophore

480 : 15 menit FAM :
0
95 : 10 menit TEXAS RED :
950 : 15 detik VIC :
40x
600 : 30 detik