GROUP 3 March 7, 2017 SCORE:

9:30 – 11:30 TTHS 0819L

Experiment No. 12
Acid – Fast Staining

I. Acid – fast Bacilli

Organism: Mycobacterium tuberculosis

Total Magnification: 1000x
Characteristics: Acid-fast cells will stain fuchsia. Non-acid-fast cells
will stain blue.

II. Flagella

Organism: Vibrio cholerae

Total Magnification: 1000x
Characteristics: Monotrichous. Gram – negative stain.

III. Endospores

Organism: Bacillus
Total Magnification: 1000x
Characteristics: Endospores will stain green. Parent cells will stain red.
 IV. Capsule

Organism: Klebsiella pneumoniae

Total Magnification: 1000x
Characteristics: The background will be dark. The bacterial cells will be
stained purple. The capsule will appear clear against the dark
background.

Reference:
Afshar, P., et. Al. Stain Protocols. Retrieved from http://www.austincc.edu/microbugz/index.php