TY - JOUR

AU - Sethi, Shruti
AU - Joshi, Alka
AU - Arora, Bindvi
AU - Bhowmik, Arpan
AU - Sharma, R. R.
AU - Kumar, Pushpendra
PY - 2020
DA - 2020/03/01
TI - Significance of FRAP, DPPH, and CUPRAC assays for antioxidant activity
determination in apple fruit extracts
JO - European Food Research and Technology
SP - 591
EP - 598
VL - 246
IS - 3
AB - Thirteen apple cultivars were analyzed for their total phenolic content,
total flavonoids, anthocyanins, ascorbic acid in methanolic extracts of both peel
and cortex fractions. Three in vitro assays (FRAP, DPPH, and CUPRAC) were used to
determine the antioxidant activity. Concentration of the phytochemicals studied
varied greatly between the apple peel and the cortex region. Peels showed ~ 2.8
times higher total phenolic content and ~ 2.68% higher flavonoid content than the
cortex. Principal component analysis could successfully explain 76.86% and 84.27%
variability in the antioxidant determinants (antioxidants/assays) in the peel and
cortex region of apple cultivars, respectively. Major contributor for antioxidant
activity in both apple peel and cortex was total flavonoid content. Cultivars ‘Well
Spur’ and ‘Oregon Spur II’ were found to be substantially rich in these two
antioxidants. The antioxidant activity was best expressed by the in vitro FRAP
assay in both the fractions. Non-hierarchical K-medoids clustering reflected the
presence of an antioxidant/ assay protocol apart from the antioxidant/assay we
considered in this study that needs further exploration to get full spectra of
antioxidant profile across apple genotypes. Based on multivariate analysis and the
concept of RACI, the FRAP antioxidant assay is recommended for determining
antioxidant activity in apples.
SN - 1438-2385
UR - https://doi.org/10.1007/s00217-020-03432-z
DO - 10.1007/s00217-020-03432-z
ID - Sethi2020
ER -