Paciente, Angelie S.

1. The color of glycogen extract is cloudy-white.

2. The alcohol has been top up when it was added into the glycogen extract, the
glycogen extract was in the bottom of the test tube while the alcohol solution is at the
top of it. In short, Glycogen is insoluble to alcohol solution because in the existence of
sodium chloride it forms a precipitate that will pin down the objective nucleic acid.

3. The difference between starch and glycogen in terms of reaction to iodine and
structure are: Starch reacts on iodine by its blue-black color which means it acts
positively, it is stored in plants as a food reserve, and is present in two forms- Amylose
and Amylopectin. On the other hand, Glycogen reacts with iodine by its reddish-brown
color which means it acts negatively, is found in animals, and is stored in the liver, and it
is made up of monomer units α-D-Glucose.

4. The unhydrolyzed glycogen has a negative reaction, no reducing sugar found, and
has a pale-blue color/sky-blue when it was tested with Fehling’s reagent because it
doesn’t have free aldehyde or keto group so they are known as non-reducing sugar and
knowing that non-reducing sugar can’t have a positive reaction for Fehling's test.

5. The hydrolyze glycogen has a positive reaction, reducing sugar, and has a brown
color when it was tested with Fehling’s reagent because it acts as a reducing agent in
alkaline solution, it has an open-chain aldehyde or ketone form in alkaline solution
where it reacts with Fehling's solution by reducing the copper ions to copper oxide.

6. The purpose of hydrolyzing the glycogen before submitting it to Fehling’s test is to

phosphate esters of glucose, be able to give a positive reducing test, and establish the
effect of concentrated HCL on glycosidic bond in glucose.