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  • XII-Bio-Expt No. 4

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    Aryan Khode
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    45 views4 pages

    XII-Bio-Expt No. 4

    Uploaded by

    Aryan Khode

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 4
    teas OO To study an identification the stages of gamete development in mouse in (mammial) i-e.'T. S. of testis and L. S of ovary through permanent slide. ae Objective. To study and identify the stages of gamete development in mouse (mammal) i-e., T.S. of testis and L.S. of ovary through permanent slide. REQUIREMENTS Permanent slide of T:S. of testis and L.S. of ovary, microscope. PROCEDURE Fix the permanent slide under the microscope. First observe it under the low power and then under high power. OBSERVATIONS TS. of Testis 1. The testis of a mouse (mammal) is covered by a thick fibrous tissue called tunica albuginea. 2. The testis consists of numerous seminiferous tubules embedded in the interstitial tissue. 3. Various types of germinal cells are present from outside towards lumen in the following sequence. ‘Spermatogonia > Spermatocytes +> Spermatids > Spermatozoa — Sperms. 4. Between the germinal cells, pyramid shaped cells called sertoli cells are present. 5, Allarge number of spermatozoa with their heads embedded in the lumen of seminiferous tubule. 6. The interstitial tissue also contain leydigs cells, which produce male sex | testosterone. VS. of Ovary 1. A mouse ovary is a solid structure bounded by germinal epithelium followed by a thick. layer of fibrous tissue, the tunica albuginia. Inert { f alle m . Fig. 11.1, A. A Part of transverse section of testis of mouse (mammal) j BB. Sectional view of a part of seminiferous tubule (enlarged) Eagnest primary flee Secondary & \

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