CLASS 12 BIOLOGY

CHAP 12 BIOTECHNOLOGY AND ITS

APPLICATIONS
1.What is transgenic organism?
2.Write 2 applications of biotechnology
3.What are probes?
4.Name the toxin produced by B.
thuringiensis
5.What is the utility of Bt-Toxin gene?
6.How inactive Bt is converted to active
form?
7.How Bt causes death of insects?
8.Name a few forms of cry gene
9.List the specific insects killed by cryIAc
and cryII Ab
10. Name the insects killed by proteins
coded by cry IIIAb and cry IIIBb
11. What is unique about transgenic
animals?
12. What is silencing of mRNA?
13. What is cDNA?
14. Mention the chemical change that
proinsulin undergoes to be able to act as
mature insulin
15. What is the application of genetically
engineered bacteria?
16. How does Rosie differ from other
cows?
17. Give characteristics of GMOs
18. List 3 critical research areas of
biotechnology.
19. Highlight the major limitations of the
earlier green revolution.
20. What is reporter or marker gene?
21. Name different transfection methods.
22. Why mice are considered most suitable
for transgenic production?
23. Define germ line gene therapy
24. Name a few useful products obtained
from animal cell lines
25. Why is insulin extracted from bacteria
rather than an animal source?
26. Why is the use of probes considered
better than conventional diagnostic tools
for disease diagnosis?
27. What is golden rice?
28. Does our blood have proteases and
nucleases?
29. How to make orally active protein?
30. What is downstream processing?