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Separation of Amino Acids by Ion-Exchange Chromatography Mixtures of amino acids can be

analyzed by first separating the mixture into its components through ion-exchange chromatography.
Amino acids placed on a cation-exchange resin (see Figure) containing sulfonate ( ) groups flow down
the column at different rates because of two factors that influence their movement: (1) ionic attraction
between the sulfonate residues on the column and positively charged functional groups on the amino
acids, and (2) aggregation of nonpolar amino acid side chains with the hydrophobic backbone of the
polystyrene resin. For each pair of amino acids listed, determine which will be eluted first from the
cation-exchange column by a pH 7.0 buffer and explain your answer.

(a) Aspartate and lysine

(b) Arginine and methionine

(c) Glutamate and valine

(d) Glycine and leucine

(e) Serine and alanine