Steps in making recombinant plasmids:

The gene of interest is isolated and cut into fragments using restriction
enzymes. They are attached to the plasmid DNA. The plasmid DNA is cut
into fragments using the restriction enzyme. Using the DNA ligase, the gene
of interest and the plasmid DNA are joined together. This results to the
recombinant DNA. The recombinant DNA is introduced to the bacterial cell
through transformation. This host follows the instructions of the foreign
recombinant DNA. Its multiplication with the foreign DNA goes on and on.
The colony of bacteria having rDNA fragments arises. These recombinant
DNA fragments will then be multiplied also. These bacterial cells which
contain the recombinant plasmid are induced to undergo gene expression
and create large amounts of their encoded protein.