pKa del grupo pH pH pH pH pH pH pH pH

funcional 1 1.96 4.25 6 7 8.18 9.67 10.53

9.67 𝑁𝐻3 + 𝐺𝑙𝑢 +1 +1 +1 +1 +1 +1 +0.5 0

4.25 R Glu 0 0 -0.5 -1 -1 -1 -1 -1

6.0 R His +1 +1 +1 +0.5 0 0 0 0

10.53 R Lys +1 +1 +1 +1 +1 +1 +1 +0.5

1.96 COOH Cys 0 -0.5 -1 -1 -1 -1 -1 -1

8.18 R Cys 0 0 0 0 0 -0.5 -1 -1

CARGA TOTAL +4 +3.5 +2.5 +0.5 0 -0.5 -1.5 -2.5

𝑝𝐾𝑎1 + 𝑝𝐾𝑎2 6 + 8.18

𝑃𝐼 = = = 7.09
2 2