ROSENBACH’S

MODIFICATION OF
GMELIN’S TES

Test Determination for

BILIRUBIN

Principle:
Bilirubin, in the presence of an
acid, is oxidized to varying
degrees.

Positive result:
Play of colors
 HARISSON SPOT TEST

Test Determination for

BILIRUBIN

Principle:
BaCl2 combines with sulfate
radicals in urine to form BaSO 4
precipitates. Bilirubin will adhere
to these molecules FeCl3, in the
presence of TCA, oxidizes
bilirubin to biliverdin
WALLACE-DIAMOND
METHOD
Positive result:
Blue to green
Test color
Determination for
UROBILINOGEN