Form No.

Research Proposal Submitted for Thesis Financial Support

Fiscal Year 2019
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1. Research Proposal’s Title
Research Title: Immobilization EDTA Functionalized Fe-Mn Binary Oxide into
Hydrochar from Green Tea Waste
Strategy Research Group: Material Science
2. Research Proposal’s Type (specific only 1 Type)
       Scientific Experiment (Master Degree)

3. Curriculum
Study step:Master Degree Major:Chemistry
Faculty of Science

4. Research’s Name
1. Student’s Name : Mr. Septian Perwira Yudha       Student ID: 6010220021
E-mail address: septianyudha92@gmail.com   Telephone’s Number:0948365741
2. Major Advisor Name: Asst. Prof. Dr. Surajit Tekasakul
E-mail address: surajit.t@psu.ac.th   Telephone’s Number: 089 462 8304
3. Approved the thesis proposal on: 24 July 2018

5. Objectives
The objective of this research are:
1. To prepare hydrochar from the green tea waste that immobilized with ethylenediaminetetraacetic
acid and Fe-Mn binary oxide (EDTA-FMHC) via hydrothermal carbonization process.
2. To characterize the obtained EDTA-FMHC.
3. To evaluate the adsorption capacity of Rhodamine B, Ibuprofen and Copper (Cu2+) using the
obtained EDTA-FMHC.
6. Background information / Significance of Research Project
This research could increase the value and usefulness of green tea waste. The obtained EDTA-
FMHC would become efficient and low-cost adsorbent for Rhodamine B, Ibuprofen and Copper
(Cu2+) removal. Furthermore, it is expected that the results of this research could be applied on an
industrial scale.
7. Research Boundaries and Methodology
The conversion of green tea waste to hydrochar that immobilized with
ethylenediaminetetraacetic acid and Fe-Mn binary oxide (EDTA-FMHC) via hydrothermal
carbonization process to remove contaminants such as Rhodamine B, Ibuprofen and Copper (Cu 2+)
from aqueous solution.

7.1 Material and Equipment

a. Green tea waste as the raw material is obtained from a coffee shop around the Faculty of
Science, Prince of Songkla University
b. Copper (II) sulfate pentahydrate (CuSO4.5H2O, 98.0%, Sigma-Aldrich)
c. Ferric chloride hexahydrate (FeCl3.6H2O, 99.0%, Loba Chemie)
d. Manganese (II) sulfate monohydrate (MnSO4.H2O, 99.0%, ANaPURE)
e. Ammonium ferrous sulfate ((NH4)2SO4.FeSO4.6H2O, 99.0%, Univar)
f. Rhodamine B (C28H31CIN2O3, 95.0%, Sigma-Aldrich)
g. Ibuprofen (C13H17NaO2, 98.0%, Sigma-Aldrich)
h. EDTA disodium salt dihydrate (99.0%, Anapure)
i. DI water (De-Ionized water)
j. Elemental Analyzer to determine chemical composition of green tea waste
k. Zetasizer to measure the zeta potensial of EDTA-FMHC
l. Vibrating sample magnetometer (VSM) to measure the magnetic properties of EDTA-FMHC
m.BET Surface Area Analyzer to measure the specific surface area, pore volume, and average pore
diameter of green tea waste and EDTA-FMHC
n. X-Ray Diffraction (XRD) to observe diffraction patterns and crystalline systems of FM/HC
o. X-ray photoelectron spectroscopy (XPS) to determine the amino-functional groups, iron and
manganese species on the surfaces of EDTA-FMHC
p. Scanning Electron Microscope–Electron Dispersive X-Ray Spectrometer (SEM-EDX) to
describe the morphology and elemental analysis of green tea waste and EDTA-FMHC
q. Transmission Electron Microscope to observe atomic structure and crystallographic information
of EDTA-FMHC
r. UV-Visible Spectrophotometer to measure the absorbance of adsorbates before and after
adsorption process

7.2 Method
7.2.1 Preparation of EDTA-FMHC
Green tea waste as the raw material is washed with tap water several times, followed by
washing using DI-water until clean and not release the color. It is dried in the oven at 110 oC for 12
h. Then 20 g of green tea waste, 4.86 g of FeCl 3.6H2O, 7.84 g of (NH4)2SO4.FeSO4.6H2O, 1.7 g of
MnSO4.4H2O and 0.93 g of EDTA are mixed together and homogenized into 100 mL of DI-water
in a beaker glass, then heated at 80oC for 2 h. After that, the mixture are transferred into a 400 mL
Teflon lined autoclave reactor. Then, 50 mL of NaOH 3 M is added into reactor and stirred at
room temperature for 1 h with strong agitation at until a stable suspension is obtained. The reactor
is heated at 220oC in the oven for 24 h (Asfaram et al., 2017; Meng et al., 2015). The obtained
samples will be filtered, then washed by acetone and DI water until clear and reach neutral pH.
The samples are dried in the oven at 60oC for 12 h. The obtained sample is labeled as EDTA-
FMHC.

7.2.2 Characterization of EDTA-FMHC

The physicochemical properties of EDTA-FMHC are characterized by Elemental Analyzer,
zetasizer, VSM, BET Surface Area Analyzer, XRD, XPS, SEM-EDX and TEM.

7.2.3 Adsorption of Rhodamine B, Ibuprofen and Copper (Cu2+)

The batch experiments are used to study about the adsorption kinetic and isotherm of all
adsorbates. The pH values of initial solution will be varied from pH of 2-6 using 0.01 M HCl and
0.01 M NaOH to investigate the effect of pH in the adsorption process. For kinetic adsorption, 50 mg
of EDTA-FMHC will be mixed with 50 mL of 100 mg/L of all adsorbates at fix pH of 3.5 and 6 for
Ibuprofen and Rhodamine B as well as Copper (Cu 2+), respectivelyThe sampling process is carried
out sequentially from 0-36 h. For isotherm studies, 50 mg of EDTA-FMHC is added into 50 mL of
each adsorbates solution, then the initial concentration of all adsorbates will be varied from 0-350
mg/L at 298, 308 and 318 K. Copper (Cu2+) is analyzed by adding EDTA as a complexing reagent to
make complex Cu2+ solution (Cu-EDTA).

7.2.4 Regeneration of EDTA-FMHC

50 mg of EDTA-FMHC is added into 50 mL of each adsorbates solution, then shaked for 36 h at
room temperature. The used adsorbent were desorbed by acetone for Rhodamine B and Ibuprofen
removal, then 0.2 M of HCl for Copper (Cu2+) removal. The adsorbents are washed with DI water
until neutral pH, then dried at 60oC for 12 h. Finally, the EDTA-FMHC will be reused to remove all
adsorbates in four successive cycles.
 8. Planning

Activity Month
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Literature review
Preparation of raw
material and chemicals
Preparation of adsorbent
Characterization of
adsorbent
Adsorption Studies
Writing a thesis
Defense of the thesis
Writing and publishing
manuscript

9. Budget of the study

Items Total Expenditure

- Expense
Analytical expense 9,000

- Material
Office items/photocopy 1,000
Scientific items 2,000

Total 12,000

10. If you receive other funding providing financial supports for research activities in thesis, please
specify the name of funding (either from faculty/ university or other external agency), sources of
funding, amount of funding and duration of funding (if any)

1. Thailand’s Education Hub for Southern Region of ASEAN Countries (TEH-AC)

   Source of funding เงินงบประมาณแผ่นดิน Fiscal 2017 Amount 520,000.00 Baht
   Amount of funding 494,000.00 Baht
   Duration of funding 2 Years 0 Months ( Duration August 01, 2017 - July 31, 2019 )

I hereby certify that while applying for this grant I have not received other fundings providing
financial supports for thesis research activities or the duration of other funding supporting for thesis has
ended. All information provided here is entirely true. If it is found that all above information is not true,
I agree to deprive of my application’s right immediately.

..............................................…Student ................................................Major Advisor

(…Mr. Septian Perwira Yudha…) (…Asst. Prof. Dr. Surajit Tekasakul…)
…………/……………../………… …………/……………../………
Note Please submit to your faculty within March 31, 2019 and the Graduate School will receive
thosse documents from faculty within April 09, 2019