2013 Livestock Catalogue-20150318-121800

L i v e st oc k
INTRODUCTION
BioNote, Inc. (now is a member of the Alere group of companies) was established in the
beginning of 2003, and is considered as a pioneer of In-Vitro Diagnostics for veterinary in
needs. BioNote delivers innovative and creative solutions for our customers improving their
vet healthcare. BioNote has own automated facility to manufacture a wide range of high
quality products developed by highly competent R&D center. BioNote has manufactured
diagnostic products in accordance with ISO 9001:ISO13485 certification, and has expanded
overseas sales network over 90 countries and has been still continuing to grow.
HISTORY
03 07
03 Anigen, Inc. established 07 Gyeonggi-do excellent medium and small
enterprises selection
11 Acquired animal medicine import license
09 H5 and general pathogenic avian influenza virus
12 Acquired animal medicine manufacturing industry
antigen diagnostic
permission
11 Name change of Anigen, Inc. to Animal Genetics, Inc.
12 Winner of the Animal medicine manufacturer self-
regulation checking system Prize
04 08
09 Acquired ISO 9001:2000 TUV certification 02 World’s first Influenza H3N2 type confirmation assay
development
07 Novel canine influenza viruses and vaccine patent
acquisition
12 Winner of the Animal medicine manufacturer self-
regulation checking system Prize
05 09
08 Moved to the new Young-tong factory 01 Anigen AIV Ab ELISA knowledge economics
department new product certification (NEP) acquisition
02 Name change of Animal genetics, Inc. to BioNote, Inc.
03 Moved to new Dongtan factory
05 Food & Drug Administration medicine manufacturing
industry permission acquisition
12 ISO 2003:13485 TUV certification acquisition Anigen
Rapid Canine Heartworm Ag Test Kit USDA licensed
06 11
01 Purchased the site for the new Dongtan factory 11. 01 Acuisition by Alere
04 Canine Distemper virus antibody diagnostic kit 12. 02 Enlarged production facility and HQ
patent acquisition
12. 04 Applied for International Patent for A Novel
04 Canine Parvovirus antibody diagnostic kit patent Canine Influenza virus and Vaccine Therefore
acquisition (PCT)
06 Venture business certification (No. 061627021-01133)

07 Technical innovation medium and small enterprises
certification (No.6065-1216)
Avian product >>
IIRapid
05 AIV Ag
06 H5 AIV Ag
07 AIV Ab
08 NDV Ag
09 IBV Ag
10 IBDV Ag
IIELISA
11 AIV Ab ELISA
12 H5 AIV Ab ELISA
13 NDV Ab ELISA
14 IBV Ab ELISA
15 IBDV Ab ELISA
IIPCR Kit for Avian disease

AIV Ag
Validated from
*VLA, Veterinary Laboratory Agency, United Kingdom
*FLI, Friedrich Loeffler Institute FLI, Germany
*CSIRO, Australian Animal Health Laboratory
*FGI «ARRIAH», Federal Center For Animal Health, Russia
Avian Influenza type A virus antigen

Influenza viruses that infect birds are called "Avian Influenza virus (AIV)" with only
influenza type A viruses infecting birds. Influenza type A viruses are divided into
subtypes based on two proteins, hemagglutinin (HA) and neuraminidase (NA), on
the surface of the virus. AIV can infect chickens, turkeys, pheasants, quail, ducks,
geese, and guinea fowl, as well as wide variety of birds. AIV is transmitted primarily
through direct contact from infected birds to healthy birds, and through indirect
contact with contaminated equipment and materials. The virus is excreted through
infected birds' feces and secretions from their noses, mouths, and eyes.
Indications
• Field monitoring of Avian Influenza virus
• Tentative diagnosis for swift control in outbreak suspected situation
• Differential diagnosis of other avian major diseases
Special Features
• Detection of all AIV type A • Validated from OIE reference laboratories
• No cross-reaction with other avian viruses • Specimen: Trachea, Kidney, Avian cloaca, Feces
• Applicable to various species • Sensitivity: 100% by farm (n=19), 77.3% by feces (n=150)
• World's first commercialized rapid test kit for AIV • Specificity: 100% vs. HA, PCR (n=1,402)
Test Procedures
Eye
Lungs Kidney
Ovary
Trachea
Crop
Heart
Oviduct
Cloaca
2 Insert swap into sample tube containing assay diluent and mix it until
sample dissolved from swap and squeeze the swap against well of tube
and then discard it.
Gall Bladder
Spleen Small Intestine

Interpretation
Liver C T
Gizzard
Pancreas Negative
4 drops
C T
20 min. Positive
1 Collect swap sample from trachea, kidney, cloaca or feces. C T S
AIV Ag
C T
C 2 1
3 Take 4 Add
Invalid
the supernatant with disposable 4~5 drops into the sample hole C T
dropper provided. with disposable dropper.
Ordering Information
Cat. No. Description Type Pack size
RG15-01 Rapid AIV Ag Multi Device 1 Test x 30/Kit
RG15-02 Rapid AIV Ag Device 1 Test x 25/Kit
Eye
Superior Larynx
Bronchial Tubes
Lungs
Ovary
Kidney
5
Oropharynx Esophagus Ceca
H5 AIV Ag Validated from
*Giessen University, Gemany
*IZS Umbria e Marche, Peruzia, Italy
Avian Influenza type A subtype 5 virus antigen

Avian influenza A viruses can be classified as low and high pathogenic form based
on the severity of the illness they cause. Avian influenza A subtype H5 and subtype
H7 viruses can be considered as "high pathogenic" strain on the basis of genetic
features of the virus and the severity of the illness. Avian influenza A subtype H5
potentially has nine different subtypes (H5N1 ~ H5N9) and these subtypes can be
highly pathogenic (HPAI) or low pathogenic (LPAI). The Influenza type A subtype H5
infection has been reported among humans and sometimes causing severe illness
and death.
Avian
Indications
• Field monitoring of Avian Influenza virus subtype H5
• Rule out low pathogenic AIV strain
Special Features
• H5 AIV detection only • Specimen: Trachea, Kidney, Avian cloaca, Feces
• No cross-reaction with other avian viruses • Sensitivity: 100% by farm (n=13), 76.6% by feces
• Applicable to various species (n=115) vs. Virus isolation
• World's first commercialized rapid test kit for AIV H5 • Specificity: 100% vs. Virus isolation, PCR (n=1,402)
• Validated from OIE reference laboratories
Test Procedures
Eye
Lungs Kidney
Ovary
Trachea
2
Oviduct
Crop Insert swap into sample tube containing assay diluent and mix it until
Heart Cloaca sample dissolved frpm swap and squeeze the swap against well of tube
Gall Bladder
Interpretation
Spleen Small Intestine C T
Liver
Gizzard
Negative
Pancreas
4 drops C T
20 min. Positive
1 Collect swap sample from trachea, kidney, cloaca or feces. C T S

H5 AIV Ag
C T
C 2 1
Invalid
C T
3 Take the supernatant with disposable
dropper provided. 4 Add 4~5 drops into the sample hole
with disposable dropper.
RG15-05 Rapid H5 AIV Ag Device 1 Test x 25/Kit
6 Eye
Bronchial Tubes
AIV Ab
Avian Influenza type A antibody
Wild birds and some poultry infected by HPAI show no clinical signs, and
their virus shedding amount is too small to be detected by an antigen capture
immunoassay. The AniGen Rapid AIV Ab Test Kit has been developed for screening
these latent AIV carriers by detecting AIV antibodies.
Indications
• Avian influenza virus antibody detection
• Field monitoring of Avian Influenza virus carrier
• Antibody detection for migratory bird surveillance
Special Features
• No cross-reaction with other avian virus positive sera • Specimen: Serum
• Applicable to various species • Sensitivity: 96% vs. HI
• The world’s first antibody for AIV rapid test Kit • Specificity: 96.5% vs. HI
Performance characteristics
• The detection limit of this kit is approximately HI titer 1:16
• Serotype validation
Anigen Rapid Avian Influenza Virus Antibody Test Kit has been validated against following antisera
H1N1 H2N3 H3N2 H3N8 H4N8 H5N1 H5N2
H5N3 H6N2 H7N1 H7N3 H7N7 H8N4 H9N2
H9N7 H10N1 H11N6 H12N5 H13N6 H14N2 H15N6
Test Procedures
Interpretation
20 min.
AIV Ab AIV Ab
1 Serum 2 1 drop of serum 3 3 drops of assay diluent
"Test result with 2 lines is a negative result and 1 line is a positive result."
RB25-01 Rapid AIV Ab Multi device 10Tests x 3/Kit
7
NDV Ag
Newcastle Disease virus antigen
Newcastle Disease (ND) is characterized by sneezing, coughing, and neurologic
sign. Mortality rate is especially very high in the young age group (about 90%).
Affected layer has poor egg quality and reduced egg production in initial phase but
usually return to normal levels within four to eight weeks. ND can cause sudden
death even in vaccinated poultry and spread rapidly to nearby flocks. Diagnosis is
usually made by virus isolation, serology and clinical signs. It has been reported
that hemagglutination inhibition (HI) test which is used widely in ND virus serology
Avian
has cross reactions with other paramyxoviruses. The Anigen Rapid NDV Ag test kit
is highly specific to NDV.
Indications
• Field monitoring of Newcastle Disease virus
Special Features
• Detection of all NDV • Specimens: Avian cloaca or trachea swab
• No cross-reaction with other avian viruses • Sensitivity: 94.7% vs. RT-PCR
• World first commercialized rapid test kit for detection of NDV • Specificity: 96.4% vs. RT-PCR
Test Procedures
Eye
Lungs Kidney
Oropharynx Ovary
Trachea
Oviduct
2
Crop Insert swap into sample tube containing assay diluent and mix it until
Heart Cloaca sample dissolved frpm swap and squeeze the swap against well of tube
Gall Bladder
Interpretation
Spleen Small Intestine
C T
Liver
Gizzard Negative
Pancreas
4 drops
C T
10 min. Positive
1 Collect swap sample from oropharynx, kidney or cloaca. C T S

NDV Ag
C T
C 2 1
Invalid

4 Add 4 drops into the sample hole C T
dropper provided. with disposable dropper.
RG15-03 Rapid NDV Ag Device 1 Test x 10/kit
8
IBV Ag
Infectious bronchitis virus antigen
Infectious bronchitis (IB) is a worldwide distributed viral disease affecting all age of
chickens. The morbidity rate is extremely high and the mortality rate is dependent
on the age of the chickens when infected, and the presence of secondary invading
organisms such as E. coli. It is highly contagious disease that entire flock can be
transmitted within one or two days, through aerosol transmission (sneezing),
contaminated organic material, drinking water and equipment. The target organs
of the virus are trachea and kidney for the respiratory strain and nephrogenic
strain respectively. It is financially important disease in poultry industry because
affected layers have decreased egg production and poor egg quality.
Indications
• Field monitoring of Infectious Bronchitis virus
Special Features
• Detection of all IBV • Specimens: Trachea, kidney, cloaca (feces)
• World first commercialized rapid test kit for IBV • Specificity: 95.2% vs. RT-PCR
Test Procedures
Eye
Lungs Kidney
Ovary
Trachea
Oviduct
2 the swab until the sample has been dissolved into the assay diluent and
Crop Insert the swab into the sample tube containing assay diluent and mix
Heart Cloaca
squeeze the swab against the well of tube and then discard it.
Gall Bladder
Interpretation
Small Intestine
Spleen C T
Liver
Gizzard Negative
Pancreas
4 drops C T
10 min. Positive
1 Collect swap sample from trachea, kidney or cloaca (feces). C T S

C T
IBV Ag
C 2 1
Invalid
C T
dropper provided. 4 Add 4 drops into the sample hole
RG15-13 Rapid IBV Ag Device 1Test x 10/Kit
9
IBDV Ag
Infectious Bursal Disease virus antigen
Infectious Bursal Disease (IBD), or Gumboro Disease, is a viral disease usually
affecting young chickens 3 to 6 weeks old, and transmitted by contaminated
feed and water. Bursa of Fabricious is the main target organ of IBDV, which is an
important organ for young chickens as an immune development. IBDV serotype
1 causes clinical disease in chickens younger than 10 weeks, with older chickens
usually showing no clinical signs. IBDV serotype 2 is widespread in turkeys and is
sometimes found in chickens and ducks. In practice, a diagnosis can be indicated
by the sudden onset of mortality in chickens between 2 and 8 weeks of age, and
Avian
the presence of distinctive lesions in the Bursa of Fabricious and accompanying

blood spots in the musculature of the breast and thigh of affected chickens
Indications
• Field monitoring of Infectious Bursal Disease virus
Special Features
• Detection of all IBDV • World first commercialized rapid test kit for IBDV
• Specimen: Bursa of Fabricious, Cloaca • Specificity: 96.6% vs. RT-PCR
Test Procedures
Eye
Lungs Kidney
Ovary
Trachea
Oviduct
Crop
Heart Cloaca
Gall Bladder
Small Intestine
Spleen
2 the swab until the sample has been dissolved into the assay diluent.
Liver Insert the swab into the sample tube containing assay diluent and mix
Gizzard
Pancreas
Interpretation
C T
Negative
4 drops
C T
10 min. Positive
C T S
IBDV Ag
C T
C 2 1
Invalid
1 Collect swap sample from Bursa of Fabricious
or cloaca (feces) 3 Take the supernatant with disposable
dropper provided. 4 Add 4 drops into the sample hole
C T
RG15-04 Rapid IBDV Ag Device 1 Test x 10/Kit
10
AIV Ab ELISA Validated from
*IZS delle Venezie, Legnaro (Padova), Italy
*University of Hokkaido, Graduated School of Veterinary Medicine
Avian Influenza type A virus antibody

Wild birds and some poultry infected by HPAI show no clinical signs, and
their virus shedding amount is too small to be detected by an antigen capture
immunoassay. The AniGen AIV Ab ELISA has been developed for screening these
latent AIV carriers by detecting AIV antibodies.
Indications
• Avian influenza virus antibody detection
• Monitoring of Avian Influenza virus carrier
• Antibody detection for migratory bird surveillance
Special Features
• No cross-reaction with other avian virus positive sera • Reading time: 45 minutes
• Applicable to various species • Sensitivity: Chicken 98.2%, Duck 97.5%, Turkey 97.1%
• Specimen: Serum, plasma or egg yolk • Specificity: Chicken 97.3%, Duck 93.8%, Turkey 100%
• No sample dilution is required
Quick Procedure
1. Prepare AIV NP antigen coated test plate
2. Add 50㎕ of controls and sample to wells Evaluation of AIV Antibody detection in egg yolk
3. Add 50㎕ of anti AIV antibody-HRP to each wells 3 days

AniGen AIV ELISA
HI test
4. Incubate plate for 30 minutes at 37℃ 1.2
5. Wash plate 6 times 1
6. Add 100㎕ of substrate (Ready to use) and incubated the wells 0.8
for 10 minutes at room temperature

0.6
7. Add 100㎕ of stopping solution Starting point of
Ab detection
0.4
8. Measure the optical density (OD) at 450 nm with reference
wavelength at 620nm 0.2
9. PI value=[1-(OD sample/mean OD negative)] x 100 0

0 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
*AniGen AIV Ab ELISA kit showed that it can detect the antibody in
egg yolk approximately 3~5 days earlier than HI test.
EB45-02 AIV Ab ELISA Microplate 480 Wells/Kit
11
H5 AIV Ab ELISA
Avian Influenza type A subtype H5 virus antibody
Wild birds and some poultry infected by HPAI subtype H5 show no clinical signs,
and their virus shedding amount is too small to be detected by an antigen capture
immunoassay. The AniGen H5 AIV Ab ELISA has been developed for screening
these latent H5 AIV carriers by detecting AIV subtype H5 antibodies.
Avian
Indications
• Antibody titer check after vaccination
• H5 AIV carrier monitoring in non-vaccinated group
• H5 AIV antibody detection for prevention program
Special Features
• No cross-reaction with other AIV subtype antibodies • Reading time: 1 hour and 45 minutes
• Species: Chicken, duck, Quail • Sensitivity: Chicken 96.9% (vs. HI)
• Specimen: Serum, Plasma, Egg yolk • Specificity: Chicken 100 % (vs. HI)
• No sample dilution is required
Quick Procedure
1. Prepare AIV H5 antigen coated test plate
2. Add 50㎕ of controls and sample to wells Serotype validation
3. Add 50㎕ of Mab-HRP (1:100 dilution in the conjugate diluent) - Anigen H5 AIV Ab ELISA detects only H5 serotype
- High sensitivity and specificity compared with HI test serotype
to all wells validation
4. Incubate plate for 90 minutes at 37℃ 120
6. Add 100㎕ of substrate solution and incubated for 15 minutes 80

PI value
at room temperature 60
7. Add 100㎕ of stop solution

PI 50
40
8. Measure the optical density (OD) at 450 nm with reference 20
wavelength at 620nm 0
9. PI value= [1-(OD sample/mean OD negative)] x 100

H1N1 H3N2 H2N9 H5N2 H5N1 H5N3 H7N7 H9N2
Serotype validation
EB45-03 H5 AIV Ab ELISA Microplate 480 Wells/ Kit
12
NDV Ab ELISA
Newcastle Disease virus antibody
Newcastle disease is a contagious bird disease affecting many domestic and
wild avian species. NDV strains can be categorized as velogenic (highly virulent),
mesogenic (intermediate virulence) or lentogenic (no virulent). Velogenic strain
is very contagious and producing severe nervous and respiratory signs and
catastrophic (90% of mortality or more). Mesogenic strain causes cough, affect
egg quality and production and result in about 10% of mortality. Lentogenic strains
produce mild signs with negligible mortality.
In acute cases, the death is very sudden, and, in the beginning of the outbreak,
the remaining birds do not seem to be sick. In flock with good immunity, however,
the signs (respiratory or gastrointestinal) are mild and progressive, and are
followed after 7 days by nervous symptoms, especially twisted heads.
Indications
• Routine monitoring of NDV
• NDV diagnosis of non-vaccinated group
Special Features
• Optimal screening method for NDV • Sensitivity: 98.4% vs. “I” commercial ELISA
• Reading time: 75 minutes • Specificity: 97.7% vs. “I” commercial ELISA
• Specimen: Plasma, Serum
Quick Procedure
1. Prepare NDV antigen coated test plate
2. Dilute test sample with sample diluents to 1:500
Antibody development after vaccination and
Do not dilute controls challenge
3. Add the 100㎕ of control sera and testing diluted samples Positive rate was 100% after 3 weeks of vaccination in both
to each well ELISA (Shade zone)
Anigen ELISA S/P ratio was gnenrally higher than “I” commercial
4. Incubate plate for 30 minutes at room temperature ELISA
6. Add 100㎕ of enzyme conjugate and incubate for 30 7
minutes at room temperature 6
7. Wash the wells 5 times 5

S/P ratio
8. Add 100㎕ of substrate solution to each well 4

Anigen
IDEXX
3
9. Incubate the wells for 15 minutes at room temperature
2
10. Add 100㎕ of stopping solution to each well
1
0
wavelength at 620nm day0
합계 :0 day7
합계 :7 day14
합계 : 14 day21
합계 : 21 day28
합계 : 28 day35
합계 : 35 day42
합계 : 42
-1
12. S/P ratio= [OD sample-mean OD negative] / [mean OD Vaccination (IB,/ND) NDV challenge
positive- mean OD negative] x 100
EB45-06 NDV Ab ELISA Microplate 480 Wells/Kit
EB45-01 NDV velo Ab ELISA Microplate 480 Wells/Kit
13
IBV Ab ELISA
Infectious Bronchitis virus antibody
Infectious bronchitis (IB) is a worldwide distributed viral disease affecting all age of
chickens. The morbidity rate is extremely high and the mortality rate is dependent
on the age of the chickens when infected, and the presence of secondary invading
organisms such as E. coli. It is highly contagious disease that entire flock can be
transmitted within one or two days, through aerosol transmission (sneezing),
contaminated organic material, drinking water and equipment. The target organs
of the virus are trachea and kidney for the respiratory strain and nephrogenic
strain respectively. It is financially important disease in poultry industry because
Avian
affected layers have decreased egg production and poor egg quality.
Indications
• Routine monitoring of IBV
• IBV diagnosis of non-vaccinated group
Special Features
• Optimal screening method for IBV • Sensitivity: 98.8% vs. “I” commercial ELISA
Quick Procedure
1. Prepare IBV antigen coated test plate
Positive rate after vaccination
2. Dilute test sample with sample diluents to 1:500 Anigen IBV ELISA shows higher positive rate and S/P ratio than “B” ELISA
Do not dilute controls Maternal antibody interference effect is shorter in Anigen than “B” ELISA
(Shade zone)
3. Add the 100㎕ of control sera and testing diluted samples
100
to each well
80
4. Incubate the wells at room temperature (18~25℃) for 30
Positive rate
60
minutes
5. Wash the wells at 5 times 40
6. Add 100㎕ of enzyme conjugate to each well 20
7. Incubate the wells at 18~25℃(Room Temperature) for 30 0

1 3 5 7 10 14 18 22
minutes and wash the wells 5 times WPI
8. Add 100㎕ of substrate solution to each well and Incubate

3.5
the wells for 15±1 minutes at room temperature (18~25℃) 3
9. Add 100㎕ of stopping solution 2.5
10. Read the absorbance of the wells with a spectrophotometer

S/P ratio
2 Anigen
IBV Ab ELISA
at 450nm with reference wavelength at 620nm 1.5 “B”
IBV Ab ELISA
11. S/P value=[OD samples - OD of negative control]/[OD 1
positive control – OD of negative control] 0.5
0
1 3 5 7 10 14 18 22
WPI
EB45-05 IBV Ab ELISA Microplate 480 Wells/Kit
14
IBDV Ab ELISA
Infectious Bursal Disease virus antibody
Infectious Bursal Disease (IBD), or Gumboro Disease, is a viral disease usually
affecting young chickens 3 to 6 weeks old, and transmitted by contaminated
feed and water. Bursa of Fabricious is the main target organ of IBDV, which is an
important organ for young chickens as an immune development. IBDV serotype
1 causes clinical disease in chickens younger than 10 weeks, with older chickens
usually showing no clinical signs. IBDV serotype 2 is widespread in turkeys and is
sometimes found in chickens and ducks. In practice, a diagnosis can be indicated
by the sudden onset of mortality in chickens between 2 and 8 weeks of age, and
the presence of distinctive lesions in the Bursa of Fabricious and accompanying
blood spots in the musculature of the breast and thigh of affected chickens
Indications
• Routine monitoring of IBDV
• IBV diagnosis of non-vaccinated group
Special Features
• Optimal screening method for IBDV • Sensitivity: 99.5% vs. “I” commercial ELISA
Quick Procedure
1. Prepare IBV antigen coated test plate
2. Dilute test sample with sample diluents to 1:500 Antibody detection after vacciantion
- Anigen IBDV Ab ELISA detects IBDV Ab 2weeks after vaccination
Do not dilute controls
3. Add the 100㎕ of control sera and testing diluted samples
to each well
4. Incubate the wells at room temperature (18~25℃) for 30
minutes
5. Wash the wells at 5 times
6. Add 100㎕ of enzyme conjugate to each well
7. Incubate the wells at 18~25℃(Room Temperature) for 30
minutes and wash the wells 5 times
8. Add 100㎕ of substrate solution to each well and Incubate
the wells for 15±1 minutes at room temperature (18~25℃)
9. Add 100㎕ of stopping solution
10. Read the absorbance of the wells with a spectrophotometer
at 450nm with reference wavelength at 620nm
11. S/P ratio=[OD450 samples - OD450 NCx] / [OD450 PCx -
OD450 NCx]
EB45-07 IBDV Ab ELISA Microplate 480 Wells/Kit
15
PCR Kit for Avian disease
Indications
• Confirm poultry diseases by antigen detection
• Differential diagnosis of difficult case shows similar signs
• Apply to clarify signs of infection in vaccinated group shows uncertain antibody titer
Avian
Special Features
• Ultimate diagnostic system for poultry infectious disease
• Optimal& unique primers and probes system guarantees highly specific result
• BioNote general PCR Detection Kits contain all components for the detection of agents
• Includes positive and negative control
• More convenient method than virus culture
• One step PCR using Taqman probe (Real-Time)
• Enables quantitative and qualitative analysis (Real-Time)
• Higher detection limit than general PCR : 100~1,000 times more sensitive (Real-Time)
List of PCR Kit for Avian disease

Product Diagnosis Cat. No.
AIV Detection Kit AIV type A (Matrix gene) PD55-01
AIV H5 Detection Kit Subtype H5 PD55-02
General
Velo NDV Detection Kit Velogenic Newcastle Disease virus PD55-08
IBDV Detection Kit Infectious Bursal Disease virus PD55-11
AIV A Real-Time Detection Kit AIV H5/N1 Duplex Detection Kit PD65-01
Velo NDV Real-Time Detection Kit High pathogenic NDV virus (Velogenic) PD65-08
Real-time IBV Real-Time Detection Kit Infectious Bronchitis Virus PD65-09

IBDV Real-Time Detection Kit Infectious Bursal Disease Virus PD65-11
Avian Mycoplasma gallisepticum, and M. iowae,
MYCO G/S Real-Time Detection Kit PD65-14
M. meleagridis , M. synoviae
16
Swine product >>
IIRapid
18 PED Ag
19 TGE Ag
20 Rota Ag
IIELISA
21 CSFV Ab ELISA
22 PRRS Ab ELISA 4.0
24 PED IgA ELISA
25 PCV-2 Ab ELSIA
IIPCR Kit for Swine disease

PED Ag
Porcine Epidemic Diarrhea virus antigen
Porcine epidemic diarrhea virus (PEDV) is a member of the coronavirus group
which causes watery diarrhea, dehydration and high mortality in suckling pigs.
Porcine Transmissible Gastroenteritis Virus (TGEV) is serologically unrelated
with PEDV, but clinically these two virus infections are difficult to differentiate.
The PEDV is transmitted by feces from infected pigs after oral ingestion.
Current available laboratory diagnosis for PED is a cryostat section - direct
immunofluorescence assay of the small intestine or colon. ELISA and RT-PCR
assays to detect viral antigens in feces or intestinal contents are useful, but require
specialized instruments and laboratory personnel. The Anigen Rapid PED Ag test
kit has been developed to provide fast and reliable test result of PED antigen in
field condition.
Indications
• Differential diagnosis of swine diarrheal disease
• PEDV field monitoring
Specifications
• No cross reaction with other etiologic agents [Poper sample amount collected by a swab]
Swine
• World's first & sole PED virus antigen rapid test kit
• Specimen : Diarrheal feces
• Sensitivity: 92% vs. RT-PCR
• Specificity: 98% vs. RT-PCR
Good Too much
Test Procedures
or
1 Collect Sample from diarrheal feces using the swap. 2 Insert swap into sample tube containing assay diluent and mix it until
sample dissolved frpm swap and squeeze the swap against well of tube
Interpretation
C T
4 drops Negative
5~10min.
C T
C T S
Positive
PED Ag
C 2 1
C T
3 Wait 30 seconds for sedimentation and

take the supernatant with disposable 4 Add 4 drops into the sample hole
with disposable dropper. C T
Invalid
dropper provided.
RG14-01 Rapid PED Ag Device 1 Test x 10/Kit
RG14-03 Rapid TGE/PED Ag Dual device 1 Test x 10/Kit
18
TGE Ag
Transmissible Gastroenteritis virus antigen
Transmissible gastroenteritis (TGE) is a viral disease of the small intestine that
causes vomiting and diarrhea in pigs of all ages. The infection spreads rapidly by
aerosol or contact exposure. Severe epidemics are more common during winter
due to survival of the virus in colder temperatures. Depending on the level of
immunity and exposure, diarrhea may be mild in some litters but severe in others.
Because TGE virus is easily spread during an epidemic by persons, animals, and
other means, fast diagnosis and special care should be taken to prevent spread to
unexposed groups of pigs and to neighboring herds. Current clinical signs in the
epidemic form of TGE usually justify a presumptive diagnosis. In the mild endemic
form, laboratory confirmation is required. The Anigen Rapid TGE test kit has been
developed to provide fast and reliable test result of TGE antigen in field condition.
Indications
• Differential diagnosis of swine diarrheal disease
• TGE field monitoring
Special Features
[Poper sample amount collected by a swab]
• No cross reaction with other etiologic agents
• World's first & sole PED virus antigen rapid test kit
• Sensitivity: 92.1% vs. RT-PCR
• Specificity: 95.2% vs. RT-PCR
Good Too much
Test Procedures
or
Interpretation
C T
Negative
4 drops
5~10min. C T
Positive
C T S
TGE Ag
C 2 1
C T

4 Add 4 drops into the sample hole Invalid
C T
take the supernatant with disposable with disposable dropper.
dropper provided.
RG14-02 Rapid TGE Ag Device 1 Test x 10/Kit
19
Rota Ag
Rotavirus antigen
Rotavirus is the most common viral causative agent of diarrhea in pigs, cattle and
dogs. Group A and B rotavirus are involved with group A being most prevalent and
clinically important, containing several serotypes of differing virulence. All ages are
susceptible. If neonatal pigs and cattle do not receive protective levels of maternal
antibody, they are likely to develop profuse watery diarrhea in 12-48 hr. More
commonly, the infection is endemic in a herd. Usually confirmatory diagnosis is
based on histologic demonstration of villous atrophy in the jejunum or electron
microscopy demonstration of virion in the intestinal contents. The methods take
long time and require high priced specialized equipment and expertise. The Anigen
Rapid Rota Ag test kit has been developed to provide fast and reliable test result of
Rota antigen in field condition.
Indications
• Differential diagnosis of bovine and swine diarrheal disease
• Rotavirus field monitoring
Specifications
• No cross reaction with other etiologic agents [Poper sample amount collected by a swab]
Swine
• World's first commercialized rapid test kit for detection of

Rotavirus antigen
• Sensitivity: 92% vs. RT-PCR
• Specificity: 99% vs. RT-PCR
Good Too much
Test Procedures
or
Interpretation
C T
Negative
4 drops
5~10min. C T
Positive
C T S
Rota Ag
C 2 1
C T

C T
take the supernatant with disposable with disposable dropper.
dropper provided.
RG18-03 Rapid Rota Ag Device 1 Test x 10/Kit
20
CSFV Ab ELISA
Classical Swine Fever virus antibody
Classical Swine Fever Virus (CSFV), previously called hog cholera virus, is a
Pestivirus in the family Flaviviridae. The CSFV is closely related to the ruminant
pestiviruses which cause Bovine Viral Diarrhea (BVDV) and Border Disease (BDV).
The virulence of CSFV strains varies widely which is leading to a wide range
of clinical signs. Highly virulent strains result in acute and severe clinical signs
including neurological signs and hemorrhages and high mortality rate. Less virulent
strains can give rise to sub-acute or chronic infections that may escape detection,
while still causing abortions and stillbirth. Herds in high risk areas are usually
serologically tested on a thorough statistical basis.
Indications
• Routine monitoring of CSFV
• Diagnosis of CSFV by antibody titration
• Titer check after vaccination
Special Features
• No cross reaction with other swine disease positive sera Correlation rates with other commercial ELISA
• Blocking ELISA which is OIE reference method for CSFV - Random 274 samples from vaccinated group
• Specimen: Plasma, Serum 'P' ELISA 'I' ELISA
• Reading time: 1 hour and 45 minutes Anigen CSFV Ab Different results
• Sensitivity: 99.3% vs. Commercial ELISA ELISA 17 samples 27 samples
• Specificity: 99.7 % vs. Commercial ELISA Correlation rate 93.8% 90.0%
Quick Procedure
1. Prepare CSFV antigen coated test plate
2. Add 50㎕ of the sample diluent into each well of the plate
3. Add 50㎕ of controls and sample to wells
4. Incubated plated for 60 minutes at 37℃
5. Wash plate 5 times
6. Add 100㎕ of enzyme conjugate(ready to use) into each well
7. Incubate the wells at 37℃ for 30 minutes
9. Add 100㎕ of substrate(ready to use) to each well and incubate for 15 minutes at room temperature
11. Measure the optical density (OD) at 450 nm with reference wavelength at 620nm
12. PI value = [1-(mean OD sample/mean OD negative control)] x 100
EB44-13 CSFV Ab ELISA Microplate 480 Wells/Kit
21
PRRS Ab ELISA 4.0
Porcine Reproductive and Respiratory Syndrome antibody
Porcine reproductive and respiratory syndrome (PRRS) is characterized by
reproductive failure of sows and respiratory problems of piglets and growing pigs.
The disease is caused by PRRS virus, currently classified as a member of family
Arteriviridae, genus Arterivirus. The primary target cell of the virus is alveolar
macrophage of pig. Two major types of the virus exist, the European (EU) and the
North America (NA) strain. The virus is primarily transmitted by contaminated
feces, urine, semen and infected pigs. High level of sanitary management system is
required because fomite infection is also possible.
Indications
• Routine monitoring of PRRS
• PRRS diagnosis of non-vaccinated group
Swine
Special Features
• No singleton reactor (false positive) • Specimen: Plasma, Serum
• Antibodies against European strain, North America • Reading time: 75 minutes
strain and Korean Strain can be detected • Sensitivity: 98.7% vs. IFA
• No cross reaction with other swine disease positive sera • Specificity: 99.7% vs. IFA
Quick Procedure
1. Preparation antigen coated micro assay plate
2. Dilute test samples and controls with sample diluents (1:39 dilution)
3. Add 100㎕ of diluted samples and controls to wells
4. Incubate plate for 30 minutes at room temperature
6. Add 100㎕ of enzyme conjugate to wells
9. Add 100㎕ of substrate solution and incubated for 15 minutes at room temperature in dark
10. Add 100㎕ of stop solution
11. Measure the optical density (OD) at 450 nm with reference wavelength at 620nm
12. S/P value=[OD sample-mean OD negative]/[ mean OD positive- mean OD negative] x 100
Sensitivity and specificity study

-Anigen PRRS Ab ELISA 4.0 has sensitivity, 98.7% and high Specificity, 99.7%
Anigen PRRS Ab ELISA 4.0
Positive Negative Total
Positive 324 4 328
IFA Negative 1 333 334
Total 325 337 662
22
Seroconversion study after challenge of PRRSV
1) Antibody response after challenge with PRRS EU, NA strainr
EU NA
4.0 4.0
3.5 3.5
3.0 3.0
2.5 2.5
S/P ratio
S/P ratio
Commercial Commercial
2.0 Kit A 2.0 Kit A
1.5 AniGen 1.5 AniGen
1.0 1.0
0.5 cut off 0.5 cut off

0.4 0.4
0.0 0.0
0 7 13 18 24 29 35 40 0 7 13 18 24 29 35 40
* Anigen PRRS Ab ELISA 4.0 can detect PRRS Ab from DPI 7 with PRRS EU and NA strain
Compare with other commercial ELISA

Product Anigen “I” Commercial vol.2 “I” Commercial vol.3
Test well No. for 1
1 Well 2 Well 1 Well
sample
Time Incubation 30 min. Incubation 30 min. Incubation 30 min.
Cut off value S/P 0.4 S/P 0.4 S/P 0.4
Singleton reactor No Yes No
AniGen PRRS Ab ELISA Kit is more sensitive than others

Detection period is earlier than "I" commercial ELISA with no singleton reactor
Days post vaccination

4.0
3.0
Mean S/P ratio
2.0 AniGen
“I” Commercial vol.2

1.0
“I” Commercial vol.3
0
-1.0
D0 D14 D35
<PRRS vaccination DPI results>
EB44-04 PRRS Ab ELISA 4.0 Microplate 480 Wells/Kit
23
PED IgA ELISA
Porcine Epidemic Diarrhea virus
Porcine epidemic diarrhea virus (PEDV) is a member of the family Coronaviridae.
PEDV causes acute enteritis in swine of all ages, and it is often fatal for neonatal
piglets.
To protect piglets from PEDV, Sow transfers immunoglobulin through colostrums
to their children until the piglets acquire adaptive immunity. And many reports
suggested that IgA is important for protection of PEDV. Anigen IgA ELISA measures
preventive anti PED-virus IgA titers of sow and predict defense capability of piglets
induce by passive antibody transfer.
Indications
• Quantitative detection of PED IgA antibody
• Screening for defensive capacity against PEDV
Special Features
Swine
• Easy sample collection • Reading Time : 1 hour and 45 minutes

• Optimal screening method for defensive capacity of PEDV • Survival rate of IgA positive confirmed group
• Specimen : Colostrums after challenge : see tables below
Quick Procedure
1. Prepare PED antigen coated test plate
Changes in composition of colostrum
2. Dispense 100㎕ of sample diluents into the all strip well
14.0
3. Dispense 10㎕ of positive, negative control and samples to 12.0
each well 10.0

Concentration
4. Incubate the plate at 37±1℃ for 60 minutes 8.0
6.0
5. Wash the plate at 5 times 4.0
IgA
6. Dispense 100㎕ of diluted enzyme conjugate to each well 2.0 IgG
7. Incubate the wells for 30 minutes at37±1℃ 0.0

7 14 21 28
Colostrum Day
8. Wash the plate at 5 times (The concentration of the IgA is higher than IgG in sow’s milk)
9. Dispense 100㎕ of substrate to each well Antibody development (in pregnant sows. 2007 research)
10. Incubate the wells for 15 minutes at room temperature ◈ Inoculated agent : cell adapted DR13 strain
◈ Specific ELISA was used for IgA detection
(18~25℃) 90
Survival rate of P.O group (IgA)
A-farm-O
11. Dispense 100㎕ of stopping solution

80 B-farm-O
70
12. Measure the optical density (OD) at 450nm with reference 60
50
Survival rate of I.M group (IgG)
A-farm-IM
B-farm-IM
30
13. Cut off value = [0.35+ the mean Negative Control absorbance] 20
10
0
Oral IM Control
Survival rate(%)
(Piglet survival rate)
(Research in Veterinary Scien ce, Song et al., 2007)
EB44-10 PED IgA Ab ELISA Microplate 480 Wells/Kit
24
PCV-2 Ab ELISA
Porcine Circovirus Type 2, or PCV2, is a very small circular‐arranged DNA virus that
belongs to the Circoviridae family. PMWS is a serious manifestation of PCV2 infection.
PMWS is characterized by severe loss of weight (wasting) and generalized lymph node
enlargement. Today we know that there are several PCV2 associated diseases (PCVAD)
and severe systemic PCV2 infection remains as the most important manifestation.
PCV2‐associated Porcine Respiratory Disease Complex (PRDC) is also a very commonly
diagnosed PCVAD in the U.S. less commonly diagnosed diseases include PCV2‐
associated Enteritis, PCV2‐ associated Reproductive Failure, and Porcine Dermatitis
and Nephropathy Syndrome (PDNS).
PCV2‐infection is widespread and essentially all pig herds are infected with PCV2 but
relatively few have PCVAD. In many cases, PCV2 infection requires a trigger such as
co-infection with other pathogens (PRRSV, Mycoplasma hyopneumoniae), immune
stimulation of the host, or other stressors to trigger PCV2 infection to progress to
PCVAD. Host genetics may also markedly affect the outcome of PCV2 infection and
there is increasing evidence of differences in virulence among PCV2 isolates.
Indications
• Diagnosis of PCV2 by antibody titration
• For prevention of PRDC, PDNS, PMWS
Special Features
• Useful massive screening method for PCV2 • Specimen: Plasma, Serum
• No cross reaction with other swine disease positive sera • Reading time: 75 minutes
• Measurement of IgG induced by orf 2 of PCV2 to predict
outcome of infection
Quick Procedure
1. Preparation antigen coated micro assay plate
2. Dilute test samples and controls with sample diluents Correlation of neutralizing antibody titer with IgG and IgM
-Neutralizing antibody (NA) titer was correlated with IgG
(1:39 dilution)
11.5 1.2
3. Add 100㎕ of diluted samples and controls to wells 10.5 1.0

Optical density
9.5
VNT50 (log2)
0.8
8.5
5. Wash plate 5 times 7.5
0.6
0.4
6. Add 100㎕ of enzyme conjugate to wells 6.5
5.5 0.2
7. Incubate plate for 30 minutes at room temperature 4.5 0
8. Wash plate 5 times 0 7 14
Days post infection

21 49 69
9. Add 100㎕ of substrate solution and incubated for 15 *NA: Green line, IgG: Red line, IgM: Blue line (Veterinary Microbiology 125 (2007) 244–255)
minutes at room temperature in dark Dynamics and viral loads in PCV inoculated pigs
10. Add 100㎕ of stop solution -NA has inverse proportion relation with viral load
11. Measure the optical density (OD) at 450 nm with reference 12.0 6
DNA copies/ml (log0)
10.0 5
VNT50 (log2)
wavelength at 620nm 8.0

6.0
4
3
12. S/P value = [OD sample-mean OD negative] / [ mean OD 4.0

2.0
2
1
positive- mean OD negative] x 100 0.0

0 7 14 21 49 69
0
Days p.i.
*NA: Green line, Viral load: Ivory bar (Veterinary Microbiology 125 (2007) 244–255)
Sensitivity and specificity study

PCV 2 Ab ELISA Microplate 480 Wells/Kit
25
PCR Kit for Swine disease
Indications
• Confirm swine diseases by antigen detection
• Differential diagnosis of difficult case shows similar signs
• To clarify signs of infection in vaccinated group shows uncertain antibody titer
Special Features
• Ultimate diagnostic system for swine infectious disease
• Optimal& unique primers and probes system guarantees highly specific result
• Includes positive and negative control
• More convenient method than virus culture
• One-step PCR using Taqman probe
• Enables quantitative and qualitative analysis
• Higher detection limit than general PCR : 100~1,000 times more sensitive
Swine
Differential Diagnosis
• BioNote *PRRS Real-Time Detection Kit
→ Useful for differentiate EU and PRRSV strain
• BioNote *SIV Real –Time Detection Kit
→ Useful for differentiate Swine Influenza Virus (Influenza A H1N1) and original Swine Influenza virus
Real-Time PCR Kit for Swine disease

Product Diagnosis Cat. No.
PCV-2 Real-Time Detection Kit Porcine Circovirus PD64-03
Porcine Reproductive and Respiratory Syndrome
PRRSV Real-Time Detection Kit PD64-04
Virus (Differential diagnosis of EU/US strain)
Real-Time CSFV Real-Time Detection Kit Classical swine fever virus PD64-05
Influenza A (H1N1) Swine
Novel Influenza A (H1N1) PD64-01
Real-Time Detection Kit
SIV Real-Time Detection Kit Original Swine Influenza virus PD64-02
26
Bovine product >>
IIRapid
28 B.Brucella Ab
29 GS.Brucella Ab
30 Bovine TB Ab
31 FMD NSP Ab
32 BoviD-5 Ag
IIELISA
33 B.Brucella Ab ELISA
34 BTB Ab ELISA 2.0
35 TB feron ELISA
37 FMD NSP Ab ELISA
B.Brucella Ab Validated from
*IZS Umbria e Marche, Peruzia, Italy
*Agence Française de Sécurité Sanitaire des Aliments, France
Brucella abortus antibody

Bovine brucellosis is commonly caused by B. abortus and less frequently by B.
melitensis, and rarely by B. suis. Humans may be infected by contact with animals
or animal products contaminated with these bacteria. Available serological tests
include the Rose Bengal, ELISA, complement fixation test and tube agglutination
test. However, these tests require sample preparation or specialized person and
equipment. The Anigen B. Brucella Ab Test Kit provides swift and accurate field
test result.
Indications
• Diagnosis of bovine brucellosis in field
• Screening for test and slaught governmental policy
• Massive screening for bovine import & export market
Special Features
• D etection of antibodies against Brucella abortus, • World’s first commercialized rapid test kit for
melintensis and suis detection of B. brucellosis
• Standardized by OIE standard sera (B.abortus 1119-3) • Specimen: Blood, Plasma, Serum, Milk
• More reliable result than rose bengal test • Sensitivity: 100% vs. ELISA
• High correlation with ELISA test • Specificity: 99.1% vs. ELISA
Advantages of Anigen Rapid B.Brucella Ab test

RBT MRT Anigen
All type (whole blood, Serum, Plasma
Specimen Serum only Milk only
and Raw milk)
Use in field No Yes Yes
Time One day 1 hour 20 minutes
Cross reaction Cross reaction with Yersinia enterocolitica No cross reaction
* RBT : Roes Bengal Test
* MRT : Milk Ring Test
Bovine
Test Procedures
Interpretation
C T
Negative
Whole blood 3 drops C T

20㎕ 20 min. Positive
C T
C T S C T S
B.brucella Ab
B.brucella Ab
C 2 1 C 2 1 Invalid
C T
1 Serum, plasma 2 Slowly add one drop (20㎕) by a capillary 3 Add 3 drops with the bottle
containing Assay diluent.
or raw milk tube of sample to the sample well.
RG23-01 Rapid B.brucella Ab Device 1 Test x 10/Kit
28
GS.Brucella Ab
Brucella melitensis antibody
Ovine and caprine brucellosis is commonly caused by B. melitensis and less
frequently by B. abortus and rarely by B. suis. Humans may be infected by contact
with animals or animal products contaminated with these bacteria. Available
serological tests include the Rose Bengal, ELISA, complement fixation test and tube
agglutination test. However, these tests require sample preparation or specialized
person and equipment. The Anigen GS. Brucella Ab Test Kit provides swift and
accurate field test result.
Indications
• Diagnosis of ovine and caprine brucellosis in field
Special Features
• Detection of antibodies against Brucella melintensis, • Specimen: Blood, Plasma, Serum, Milk
abortus and suis • Sensitivity: 98% vs. ELISA
• More reliable result than rose bengal test • Specificity: 100% vs. ELISA
• High correlation with ELISA test
• World’s first commercialized rapid test kit for detection of
B. brucellosis
Advantages of Anigen Rapid GS.Brucella Ab test

RBT MRT Anigen
All type (whole blood, Serum, Plasma
Specimen Serum only Milk only
and Raw milk)
Use in field No Yes Yes
Time One day 1 hour 20 minutes
Cross reaction Cross reaction with Yersinia enterocolitica No cross reaction
* RBT : Roes Bengal Test
* MRT : Milk Ring Test
Test Procedures
Interpretation
C T
Negative
Whole blood 3 drops C T
20㎕ 20 min. Positive
C T
C T S C T S
GS.brucella Ab
GS.brucella Ab
Invalid
C 2 1 C 2 1
C T
1 Serum, plasma 2 Slowly add one drop (20㎕) by a capillary 3 Add 3 drops with the bottle
containing Assay diluent.
or raw milk tube of sample to the sample well.
RB23-06 Rapid GS.brucella Ab Device 1 Test x 10/Kit
29
Bovine TB Ab
Mycobacterium bovis antibody
Bovine tuberculosis is a chronic infectious disease caused by Mycobacterium
bovis. Bovine tuberculosis infection is usually diagnosed in the live animal on the
basis of delayed hypersensitivity reactions. Subclinical infection is common and
clinical signs are non-specific in many cases. Sensitive and accurate diagnostic
tool has been required because purified protein derivative (PPD) test has been
known 65.6% sensitivity (Wood et al.,Vet. Microbiol, 40:125-135. 1994) and other
mycobacterium infection (eg; M.avium) should be distinguished with this method.
The MPB 70 protein was revealed to be a highly species specific protein secreted
by Mycobacterium bovis. Anigen rapid bovine TB Ab test kit has been developed
to using MPB70 protein on the basis of immunochromatographic assay method.
Indications
• Search out BTB carrier in field
• Screening for test and slaught governmental policy Immune development of bovine tuberculosis
• First step for massive screening instead of PPD in short- Antibody Response
handed situation Tuberculin Skin Test
• Combine with PPD or IFN-γ assay in eradication system
Immune Response
Gamma Interferon
Test (IFN-γ)
Anergy
Special Features
• No cross reaction against other mycobacterium species (M. avium) Anigen TB feron ELISA Test Kit
PPD Test
• Easy to use, saving time and labor

Anigen Rapid Bovine TB Ab Test Kit
• Specimen: Blood, Plasma, Serum Anigen Rapid BTB Ab Test Kit detects mid to late (anergy) stage of
TB affected cattle by immunochromatographic assay (Aplicable in
• Sensitivity: 81.7% vs. PPD test, 84.2% vs. “E” commercial ELISA field assay)
• Specificity: 91.4% vs. PPD test, 84% vs. “E” commercial ELISA Generally, M.bovis Ab is detectable 90 to 100 days after infection
Test Procedures
Bovine
3 drops + 1 drop
1 min.
2 3 Add 1 drop of whole blood into the

Whole blood
Add 3 drops of whole blood
diluent into the test tube. test tube and wait for 1 minutes to mix it.
Interpretation
C T
Negative
Plasma or serum
1 Whole blood : Start from number (2) 1 drop (10㎕)

3 drops C T
Plasma or serum : Start from number (4) 20 min. Positive
C T
BTB Ab
C T
BTB Ab
C T
S
S
Invalid
4 Add 1 drop of the mixed sample 5 Add 3 drops of developing buffer. C T
and wait for another 1 minute.
RB23-02 Rapid Bovine TB Ab Device 1 Test x 10/Kit
30
FMD NSP Ab
Food and mouth disease virus antibody
Foot-and-mouth disease (FMD) is a highly contagious viral infection primarily
of cloven-hoofed domestic animals, such as cattle, pigs, sheep, goats, deer,
and water buffalo. In many countries the disease is controlled by vaccinations
that consist of (partly) purified structural proteins (SP) of the FMD virus, and
therefore vaccinated animals only elicit antibodies directed against the structural
proteins. Non structural protein (NSP) is expressed only by replicating viruses,
and inactivated vaccines are purified to remove the cellular proteins and NSP.
Therefore, only animals that have been infected with wild type develop antibodies
against NSP. It is important to differentiate SP and NSP antibodies in countries that
use vaccination to control FMDV outbreaks to discriminate wild type infections
and immune response to vaccination.
Indications
• To discriminate between infection and vaccination
• For field diagnosis of FMD in non-vaccination herds
Special Features
• No cross reaction with vaccinated group
• Specimens: Blood, Plasma, Serum
• Applicable to all artiodactyl mammal (Cattle, Sheep, Goat, Pig)
• Sensitivity: 95.4% vs. Commercial ELISA
• Specificity: 100% vs. Commercial ELISA
Test Procedures
3 drops + 1 drop 1 min.
2 3 Add 1 drop of whole blood into the

Whole blood
Add 3 drops of whole blood
diluent into the test tube. test tube and wait for 1 minutes to mix it.
Interpretation
C T
Negative
Plasma or serum
1 Whole blood : Start from number (2)

1 drop (10㎕)
3 drops
C T
Positive
Plasma or serum : Start from number (4)
20 min.
C T
FMD NSP Ab
FMD NSP Ab
C T C T
S
S
Invalid
C T
4 Add 1 drop of the mixed sample 5 Add 3 drops of developing buffer.

and wait for another 1 minute.
RB28-02 Rapid FMD NSP Ab Device 1 Test x 10/Kit
31
BoviD-5 Ag
Bovine Rota, Corona, E.coil K99, Cryptosporidium antigen
Newborn calves are susceptible to neonatal calf diarrhea especially during their
first 3~4 weeks of life. Bacteria, viruses and parasites, by attacking the lining of
the calf`s intestine, give rise to diarrhea. It is one of the major financial damage
factor in bovine farm, because infected one decreases the absorption of essential
nutrients from milk and leads to weight loss and dehydration. Generally, calf
diarrhea is considered as an emergency situation requires swift diagnosis and
rehydration. Rapid test kit, one of the key feature is “rapidity” of test result, is ideal
candidate for this work.
Indications
[Poper sample amount collected by a swab]
• For differential diagnosis of calf diarrheal disease
• Calf diarrhea etiologic agent monitoring in field
• For immediate treatment required case
Special Features Not enough Good Too much
• Useful tool for ruling out the cause of calf diarrhea Sensitivity Specificity
• Specimen : Diarrheal feces Cryptosporidium 98.2% 99.0%
Rotavirus 99.0% 98.0%
• No cross reaction with other etiologic agents of calf diarrhea vs.PCR
Coronavirus 98.4% 98.0%
• Sensitivity and Specificity : see table below E.coil K 99 Ag 97.8% 99.0%
Giardia lamblia 92.1% 99.1% vs.ELISA
Test Procedures
Bovine
or
1 Collect the samples from canine feces using the swab. 2 Insert swap into sample tube containing assay diluent and mix it until
Interpretation
4 drops C T
Negative
5~10min. C T
Positive
Giardia Ag
C T

take the supernatant with disposable with disposable dropper. C T
dropper provided.
Rapid BoviD-5 Ag Device 1 Test x 10/Kit
32
B.Brucella Ab ELISA 2.0
Brucella abortus antibody
Bovine brucellosis is commonly caused by Brucella abortus and less frequently
by B. melitensis, and rarely by B. suis. Humans may be infected by contact with
animals or animal products contaminated with these bacteria. Serological tests
include the Rose Bengal, ELISA, complement fixation test and tube agglutination
test. Anigen B.brucella Ab ELISA Test Kit is a serology provides high sensitivity and
specificity with bovine milk and serum samples.
Indications
• Diagnosis of bovine brucellosis in lab
Special Features
• Fully meets the requirement of EU Council Directive and the (1)Study in serum Specimens :
OIE Manual Total 271 of positive and negative cow serum from KNVRQS*
• Standardized by OIE standard sera (B.abortus 1119-3) AniGen ELISA
• No cross reaction with yersinia enterocolitica + -
Other commercial + 127 0 127
• Specimen: plasma, serum, milk
ELISA - 3 141 144
• Reading time: 1 hour and 45 minutes 130 141
• Sensitivity: serum 100%, milk 100% (Vs Commercial ELISA)
• Specificity: Serum 97.9 %, milk 99.1% (Vs Commercial ELISA) Reseult
Sensitivity 100%
Specificity 97.9%
Quick Procedure
1. Prepare LPS coated test plate
(2) Study in raw milk Specimens :
2. Dilute test samples 1:50 with sample diluents Total 271 of positive and negative cow milk from KNVRQS*
(Do not dilute controls and Milk) AniGen ELISA
3. Add 100㎕ of positive control, negative control, and diluted + -
test samples Other commercial + 68 0 68
ELISA - 1 116 117
4. Incubated plate for 60 minutes at 37℃
69 116
6. Add 100㎕ of diluted enzyme conjugate to wells Reseult
7. Incubate plate for 30minutes at 37℃ Sensitivity 100%
8. Wash plate 5 times Specificity 99.1%
9. Add 100㎕ of mixed substrate (Ready to use) to each well and
incubated for 15 minutes at room temperature
wavelength at 620nm
12. %P value=[OD sample/mean OD positive] x 100
EB43-01 B.brucella Ab ELISA Microplate 480 Wells/Kit
33
TB feron ELISA
Gamma interferon for Mycobacterium bovis
bovis. The infection is often subclinical; even present, clinical signs are not
specifically distinctive of this disease and might include weakness, anorexia,
emaciation, enlargement of lymphnodes, and cough, particularly routine
screening although it time and labor intensive. The Anigen TB-feron is an
indirect Enzyme Linked Immunosorbent Assay for the quantitative detection of
interferon gamma (IFN-γ). IFN-γ assay is based on the fact that an animal has
blood lymphocytes which can memorize stimulating antigen immunologically
when it is stimulated by exogenous or endogenous antigens. When an antigen is
added to the blood from primed animal within a tube, antigen specific effector/
memory T cell is rapidly re-stimulated to produce IFN-γ, the cytokine, which is
used as specific marker in cell-mediated immune response (recall response). It
is an alternative test method of intradermal skin test (PPD) because of its easy
procedure and better sensitivity compared to the skin test.
Indications Special Features

• Quantitative detection of interferon gamma in bovine • High reliable result than PPD
plasma • OIE standard for BTB diagnosis
• Screening for test and slaught governmental policy • Good correlation with other IFN-γ assay
• First step for massive screening instead of PPD in short- • Provide antigens for sensitization
handed situation • Specimen : Stimulated plasma
• Combine with ELISA or Rapid test in eradication system • Reading time : 2 hours
• Concordance rate: 100% vs. “B” commercial ELISA
Quick Procedure
1. Add 100㎕ of the sample diluents to each wells
2. Add 50㎕ of controls and each of prepared samples
(Bovine PPD stimulated plasma Avian PPD stimulated plasma, PBS stimulated plasma) to each wells
3. Incubate the wells at 37℃ for 30 minutes
4. Wash the wells at 5 times
5. Add 100㎕ of prepared amplification conjugate to each wells
Bovine
6. Repeat step3 and 4

7. Add 100㎕ of prepared enzyme conjugate to each wells
8. Repeat step3 and 4
9. Add 100㎕ of substrate to each wells
10. Incubate the wells for 30 minutes at room temperature
11. Add 100㎕ of stopping solution to each well. Reading W/L 450nm (Ref. W/L 620nm)
12. OD value: OD bovine PPD-PBS and OD bovine PPD-avian PPD
Sample preparation
Stimulating antigen
Overnight Take TB feron
+ (Sensitization should be performed within
30 hours after blood collection)100㎕
incubation
for 16 hours
Centrifuge supernatant
liquid
ELISA
testing
- Bovine PPD
- Avian PPD
Plasma 3ml - PBS (Negative control)
34
Field benefits of the IFN-γ assay
• Animals retested as often as required (no interference with the immune status of animal)
• Double handing of cattle avoided
• Better sensitivity compared to skin test
• Reduced need for comparative intradermal test since both avian and bovine PPDs are used
Lab benefits of the IFN-γ assay

• Results obtained within 24 hours
• As a lab test is subject to quality control, standard procedures and objective interpretation
• Suitably adapted to epidemiological characteristics of the territor
Immune development of bovine tuberculosis

Antibody Response
Tuberculin Skin Test
Immune Response
Gamma Interferon
Test (IFN-γ)
Anergy
Anigen TB feron ELISA Test Kit
PPD Test
Anigen BTB Ab ELISA 2.0 Test Kit
Anigen TB feron ELISA detects early to mid stage of TB affected cattle by cellular immunity measurement
Performance
1. Comparative study with other IFN test
Anigen TB feron
Total
(-) (+)
(-) 87 0 87 Correlation 100% 142/167
"B" ELISA
(+) 0 12 12 Sensitivity 100% 12/12
Total 87 12 99 Specificity 100% 87/87
2. Comparative study with skin test (PPD)

Anigen TB feron
Total
(-) (+)
(-) 50 0 50 Correlation 85% 142/167
PPD
(+) 25 92 117 Sensitivity 78.6% 92/117
Total 75 92 167 Specificity 100% 50/50
EG38-01 TB feron ELISA Microplate 480 Wells/Kit (150 tests)
35
BTB Ab 2.0 ELISA
Mycobacterium bovis antibody
bovis. Bovine tuberculosis infection is usually diagnosed in the live animal on the
basis of delayed hypersensitivity reactions. Subclinical infection is common and
clinical signs are non-specific in many cases. Sensitive and accurate diagnostic
tool has been required because purified protein derivative (PPD) test has been
known 65.6% sensitivity (Wood et al.,Vet. Microbiol, 40:125-135. 1994) and other
mycobacterium infection (eg; M.avium) should be distinguished with this method.
The MPB 70 protein was revealed to be a highly species specific protein secreted
by Mycobacterium bovis. Anigen BTB Ab ELISA 2.0 test kit has been developed
to using MPB70 protein on the basis of Enzyme Linked Immunosorbent Assay
method. The serological diagnostic test is suitable for massive screening of bovine
tuberculosis to detect mid to late (anergy) state cattle.
Indications
• Search out BTB carrier
• First step for massive screening instead of PPD in short-handed situation
• Combine with PPD or IFN-γ assay in eradication system
Special Features
• No cross reaction against other mycobacterium species (M. avium) • Reading time: 75 minutes
• Quantitative detection of M. bovis antibody • Specimen: Plasma, Serum
• The world’s first reliable BTB Ab ELISA for serological diagnosis • Sensitivity: 88.1% vs. PPD test
• Specificity: 99.2% vs. PPD test
Quick Procedure
1. Prepare BTB antigen coated test plate
Immune development of bovine tuberculosis
Bovine
2. Add 50㎕ of controls and sample to wells

3. Add 50㎕ of M. bovis antigen-HRP to each well Tuberculin Skin Test
Antibody Response
4. Incubate plate for 60 minutes at 37℃

Immune Response

Gamma Interferon
Test (IFN-γ)
Anergy
6. Add 100㎕ of mixed substrate solution (Ready to use)

to each well and incubated for 15 minutes at room
temperature
7. Add 100㎕ of stopping solution to each well
Anigen TB feron ELISA Test Kit
PPD Test

Anigen BTB Ab ELISA 2.0 Test Kit
wavelength at 620nm Anigen BTB Ab ELISA 2.0 detects mid to late (anergy) stage of TB
affected cattle by antibody titer check againt TB (Laboratory method
9. S/P value=[OD sample-mean OD negative)] / [mean OD for antibody check)
positive – mean OD negative] x 100 Generally, M.bovis Ab is detectable 90 to 100 days after infection
EB43-04 BTB AbELISA 2.0 Microplate 480 Wells/Kit
36
FMD NSP Ab ELISA Validated from
*FGI «ARRIAH», Federal Center For
Animal Health, Russia
Food and mouth disease virus antibody

Foot-and-mouth disease (FMD) is a highly contagious viral infection primarily
of cloven-hoofed domestic animals, such as cattle, pigs, sheep, goats, deer,
and water buffalo. In many countries the disease is controlled by vaccinations
that consist of (partly) purified structural proteins (SP) of the FMD virus, and
therefore vaccinated animals only elicit antibodies directed against the structural
proteins. Non structural protein (NSP) is expressed only by replicating viruses,
and inactivated vaccines are purified to remove the cellular proteins and NSP.
Therefore, only animals that have been infected with wild type develop antibodies
against NSP. It is important to differentiate SP and NSP antibodies in countries that
use vaccination to control FMDV outbreaks to discriminate wild type infections
and immune response to vaccination.
Indications
• Discriminate sera between infection and vaccination
• Diagnosis of FMD in non-vaccination herds
Special Features
• Differential test of FMD infected or vaccinated • Species: Cattle, Sheep, Goat, Pig
• High accuracy equivalent to a world standard ELISA kit • Specimen: Plasma, Serum
• Easy test procedure: No serum pre-dilution required • Reading time : 1 hour and 45 minutes
• Cost effective: No requirement for an uncoated • Sensitivity: Cattle 93.8%
microplate for serum pre-dilution • Specificity: Cattle 99.9%, Pig 99.9%, Sheep& goat 100%
Quick Procedure
1. Prepare FMD NSP antigen coated test plate
2. Add 50㎕ of controls and sample to wells Reactivity of AniGen FMD NSP Ab ELISA for sera originated
from vaccinated animals
3. Add 50㎕ of diluted enzyme conjugate to wells
AniGen FMD NSP Ab ELISA detects antibodies against nonstructural
4. Incubate plate for 90 minutes at 37℃ protein the from 7 days to 7 months after infection
(*Experimentally contact challenge animal group, The performance
5. Wash plate 6 times evaluation was performed in OIE FMD Ref. Laboratory)
6. Dispense100㎕ of substrate (Ready to use) each well and 100
incubate for 15 minutes at room temperature 80
7. Add 100㎕ of stopping solution 60 AniGen
8. Measure the optical density (OD) at 450 nm with reference 40 Checkit
9. PI value=[1-(OD sample/mean OD negative)] x 100 0

5 days 7 days 14~26 days 7 months
AniGen 0% 76.1% 93.8% 50%

Checkit 0% 38.3% 93.8% 20%
EB48-01 FMD NSP Ab ELISA Microplate 480 Wells/Kit
37
Current available product list
Rapid
Species Product Cat. No. Type Packing Size Description Sample
Rapid CPV Ag Test Kit RG11-01 Device 1Test X 10/Kit Detection of Canine Parvovirus antigen Feces
Rapid Canine Heartworm Ag Test Kit 2.0 RG11-02 Device 1Test X 10/Kit Detection of Canine Heartworm antigen Whole blood, plasma or serum
Rapid CDV Ag Test Kit RG11-03 Device 1Test X 10/Kit Detection of Canine Distemper virus antigen Conjunctiva, feces, saliva, urine, plasma, or serum
Rapid CCV Ag Test Kit RG11-04 Device 1Test X 10/Kit Detection of Canine Coronavirus antigen Feces
Rapid CPV Ag/CCV Ag Test Kit RG11-05 Dual device 1Test X 10/Kit Detection of Parvovirus antigen/Coronavirus antigen Feces
Rapid CIV Ag Test Kit RG11-07 Device 1Test X 10/Kit Detection of Canine influenza virus antigen Conjunctiva and nasal swab
Rapid CDV Ag/CAV Ag Test Kit RG11-08 Dual device 1Test X 10/Kit Detection of Distemper antigen/Canine adenovirus antigen Conjunctiva and nasal swab
Rapid CIRD-3 Ag Test Kit RG11-09 Dual device 1Test X 10/Kit Detection of Distemper antigen/Canine adenovirus/Canine influenza virus antigen Conjunctiva and nasal swab
Rapid CDV Ag/CIV Ag Test Kit RG11-10 Dual device 1Test X 10/Kit Detection of Distemper antigen/Canine Influenza antigen Conjunctiva and nasal swab
Rapid CPV/CCV/Giardia Ag Test Kit RG11-12 Triple Device 1Test X 5/Kit Detection of Parvovirus antigen/Coronavirus antigen/ Giardia antigen Feces
Rapid Rabies Ag Test Kit RG18-01 Device 1Test X 10/Kit Detection of Rabies virus antigen Brain homogenates
PET Rapid Rota Ag Test Kit RG18-03 Device 1Test X 10/Kit Detection of Rotavirus antigen in bovine, porcine, canine Diarrheal faeces
Animal Rapid Giardia Ag Test kit RG18-04 Device 1Test X 10/Kit Detection of Giardia antigen Feces
Rapid CPV Ab Test Kit RB21-01 Device 1Test X 10/Kit Titration of Parvovirus antibody for vaccination & prognosis Whole blood, plasma or serum
Rapid CDV Ab Test Kit RB21-02 Device 1Test X 10/Kit Titration of Distemper virus antibody for vaccination & prognosis Whole blood, plasma or serum
Rapid Canine brucella Ab Test Kit RB21-03 Device 1Test X 10/Kit Detection of Brucella canins antibody Whole blood, plasma or serum
Rapid Leishmania Ab Test Kit 2.0 RB21-04 Device 1Test X 10/Kit Detection of Leishmania antibody Whole blood, plasma or serum
Rapid E.canis Ab Test Kit RB21-05 Device 1 Test X 10/Kit Detection of Ehrichia canis antibody Whole blood, plasma or serum
Rapid FeLV Ag Test Kit RG12-01 Device 1Test X 10/Kit Detection of Feline Leukemia virus antigen Whole blood, plasma or serum
Rapid FPV Ag Test Kit RG12-03 Device 1Test X 10/Kit Detection of Feline Panleukopaenia virus antigen Feces
Rapid FIV Ab/FeLV Ag Test Kit RC12-04 Dual Device 1Test X 10/Kit Detection of Feline Leukemia virus antigen and Feline Immunodeficiency virus antibody Whole blood, plasma or serum
Rapid FCoV AgTest Kit RG12-05 Device 1Test X 10/Kit Detection of Feline Corona virus antigen Feces
Rapid FIV Ab Test Kit RB22-01 Device 1Test X 10/Kit Detection of Feline Immunodeficiency virus antibody Whole blood, plasma or serum
Rapid FCoV Ab Test Kit RB22-03 Device 1Test X 10/Kit Detection of Feline Corona virus antibody Whole blood, plasma or serum
Vet Urocolor 10 (50) VU51-01 Strip 50 Strip/Bottle Reagent Strips for Animal Urinalysis Urine
Vet Urocolor 10 (100) VU51-02 Strip 100 Strips/Bottle Reagent Strips for Animal Urinalysis Urine
Vet UroMeter 120 UM0120 Analyzer Set Urine chemistry analyzer Urine
Rapid Bovi D-4 Ag Test Kit RG13-01 Pento Device 1Test x 10/Kit Detection of Bovine Rotavirus, Coronavirus and E.coli K99, Cryptosporidium antigen field infected Diarrheal faeces
Rapid Bovi D-5 Ag Test Kit RG13-02 Pento Device 1Test x 10/Kit Detection of Bovine Rotavirus, Coronavirus and E.coli K99, Cryptosporidium, Giardia antigen field infected Diarrheal faeces
Rapid Bovine Coronavirus Ag Test Kit RG13-03 Device 1Test x 9/Kit Detection of Bovine Coronavirus antigen field infected Diarrheal faeces
Rapid Cryptosporidium Ag Test Kit RG13-04 Device 1Test x 10/Kit Detection of Bovine Cryptosporidium antigen field infected Diarrheal faeces
Rapid E.coli k99 Ag Test Kit RG13-05 Device 1Test x 10/Kit Detection of Escherichia coli k99 antigen field infected Diarrheal faeces
Rapid Rota Ag Test Kit RG18-03 Device 1Test x 10/Kit Detection of Rotavirus antigen in bovine, porcine, canine Diarrheal faeces
Rapid Bovine brucella Ab Test Kit RB23-01 Device 1Test x 10/Kit Detection of Brucella abortus antibody Whole blood, plasma, serum or milk
Rapid Bovine TB Ab Test Kit RB23-02 Device 1Test x 10/Kit Detection of Mycobacterium bovis antibody Plasma, serum
Rapid GS.Brucella Ab Test Kit RB23-06 Device 1Test x 10/Kit Detection of Brucella antibody in goat sheep Whole blood, plasma, serum or milk
Rapid FMD NSP Ab Test Kit RB28-02 Device 1Test x 10/Kit Detection of Foot and mouth disease virus antibody field infected Whole blood, plasma or serum
Rapid PED Ag Test Kit RG14-01 Device 1Test X 10/Kit Detection of Porcine Epidemic Diarrhea virus antigen Diarrheal faeces
Rapid TGE Ag Test Kit RG14-02 Device 1Test X 10/Kit Detection of Transmissible Gastroenteritis virus antigen Diarrheal faeces
Industrial
Rapid TGE Ag/PED Ag Test Kit RG14-03 Dual device 1Test X 10/Kit Detection of TGE Ag and PED Ag Diarrheal faeces
Animal
Rapid SIV Ag Test Kit RG14-04 Device 1Test X 10/Kit Detection of Swine Influenza virus antigen Nasal fluid or tracheal swab and lung tissue
Rapid PED Ag/Rota Ag Test Kit RG14-05 Dual device 1Test X 10/Kit Detection of PED antigen and Rotavirus antigen in pig Diarrheal faeces
Rapid Rota Ag Test Kit RG18-03 Device 1Test X 10/Kit Detection of Rotavirus antigen in bovine, porcine, canine Diarrheal faeces
Rapid AIV Ag Test Kit RG15-01 multi Device (30T) 1Test x 30/Kit Detection of Avian influenza type A virus antigen Diarrheal faeces, Cloaca Feces, or Trachea
Rapid AIV Ag Test Kit RG15-02 Device (25T) 1Test x 25/Kit Detection of Avian influenza type A virus antigen Diarrheal faeces, Cloaca Feces, or Trachea
Rapid NDV Ag Test Kit RG15-03 Device 1Test x 10/Kit Detection of Newcastle Disease virus antigen Cloaca feces, Trachea
Rapid IBDV Ag Test Kit RG15-04 Device 1Test x 10/Kit Detection of Infectious Bursal Disease virus antigen Bursa of Fabricious, Feces
Rapid H5 AIV Ag Test Kit RG15-05 Device 1Test x 25/Kit Detection of avian influenza type A subtype H5 virus antigen Diarrheal faeces, Cloaca Feces, or Trachea
Rapid AIV type A/H5 AIV Ag Test Kit RG15-06 Dual device 1Test x 10/Kit Detection of avian influenza type A and H5 antigen Diarrheal faeces, Cloaca Feces, or Trachea
Rapid AIV Ag/NDV Ag Test Kit RG15-09 Dual device 1Test x 10/Kit Detection of avian influenza type A and NDV antigen Diarrheal faeces, Cloaca Feces, or Trachea
Rapid IBV Ag Test Kit RG15-13 Device 1Test x 10/Kit Detection of Infectious Bronchitis virus antigen Diarrheal faeces, Cloaca Feces, or Trachea
Rapid AIV Ab Test Kit RB25-01 Multi device 10Tests x 3/Kit Detection of Avian Influenza Virus Type A antibody Plasma, serum
ELISA
Bovine Brucella Ab ELISA EB43-01-(96) Microplate 480 (96) wells/Kit Detection of Brucella abortus antibody Serum
BTB Ab ELISA 2.0 EB43-04-(96) Microplate 480 (96) wells/Kit Detection of Mycobacterium bovis antibody Serum
FMD NSP Ab ELISA EB48-01-(96) Microplate 480 (96) wells/Kit Detection of Foot-and-mouth disease virus antibody field infected in cattle, pig, goat, sheep Serum, Plasma
TB feron ELISA EB38-01-(96) Microplate 480 (96) wells/Kit Detection of Gamma interferon for Mycobacterium bovis Plasma
CSFV Ab ELISA EB44-13-(96) Microplate 480 (96) wells/Kit Detection of Classical swine fever virus antibody for routine screening Serum, Plasma
PRRS Ab ELISA 4.0 EB44-04-(96) Microplate 480 (96) wells/Kit Deteciton of Porcine Reproductive and Respiratory Syndrome (PRRS) antibody Serum, Plasma
PED IgA ELISA 4.0 EB44-10-(96) Microplate 480 (96) wells/Kit Detection of Porcine epidemic diarrhea virus IgA antibodies in pig Colostrum
AIV Ab ELISA EB45-02-(96) Microplate 480 wells/Kit Detection of Avian influenza type A virus antibody in chicken, duck, goose Serum
H5 AIV Ab ELISA EB45-03-(96) Microplate 480 wells/Kit Detection of Avian influenza virus subtype H5 antibody in chicken, duck, goose Serum
NDV Velo Ab ELISA EB45-01-(96) Microplate 480 wells/Kit Detection of Newcastle Disease velogenic virus antibody in chicken Serum
IBV Ab ELISA EB45-05-(96) Microplate 480 wells/Kit Detection of Infectious Bronchitis virus antibody in chicken Serum
NDV Ab ELISA EB45-06-(96) Microplate 480 wells/Kit Detection of Newcastle Disease virus antibody in chicken Serum
Conventional PCR
AIV Detection Test Kit PD55-01-(20) Conventional PCR 96 (24) reactions/Kit Detection of AIV type A Diarrheal faeces, Cloaca Feces, or Trachea
H5 AIV Detection Test Kit PD55-02-(20) Conventional PCR 96 (24) reactions/Kit Detection of AIV subtype H5 Diarrheal faeces, Cloaca Feces, or Trachea
Industrial
Animal
Lento NDV Detection Test Kit PD55-07-(20) Conventional PCR 96 (24) reactions/Kit Detection of low pathogenci NDV virus (Lentogenic) Cloaca feces, Trachea
Velo NDV Detection Test Kit PD55-08-(20) Conventional PCR 96 (24) reactions/Kit Detection of high pathogenic NDV virus (Velogenic) Cloaca feces, Trachea
IBDV Detection Test Kit PD55-11-(20) Conventional PCR 96 (24) reactions/Kit Detection of Infectious Bursal Disease Virus Bursa of Fabricious, Feces
CDV Detection Test Kit PD51-03-(20) Conventional PCR 96 (24) reactions/Kit Detection of Canine Distemper Virus Conjunctiva, feces, saliva, urine, plasma or serum
PET
Leishmaniasis Detection Test Kit PD51-04-(20) Conventional PCR 96 (24) reactions/Kit Detection of Leishmania Whole blood, plasma of serum
Animal
E.canis Detection Test Kit PD51-05-(20) Conventional PCR 96 (24) reactions/Kit Detection of E.canis Whole blood, plasma of serum
Real-Time PCR
AIV A Real-Time Detection Test Kit PD65-01-(20) Real-Time PCR 96 (24) reactions/Kit Detection of AIV type A Diarrheal faeces, Cloaca Feces, or Trachea
H5 AIV Real-Time Detection Test Kit PD65-02-(20) Real-Time PCR 96 (24) reactions/Kit Detection of AIV subtype H5 Diarrheal faeces, Cloaca Feces, or Trachea
REV Real-TIme Detection Test Kit PD65-05-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Reticuloendotheliosis Virus Blood, Tissue (Scab, Pharyngeal lesions)
MDV Real-TIme Detection Test Kit PD65-06-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Marek's disease virus Tissue (Spleen), Buffy coat, Lymphoma cell
Lento NDV Real-Time Detection Test Kit PD65-07-(20) Real-Time PCR 96 (24) reactions/Kit Detection of low pathogenci NDV virus (Lentogenic) Cloaca feces, Trachea
Velo NDV Real-Time Detection Test Kit PD65-08-(20) Real-Time PCR 96 (24) reactions/Kit Detection of high pathogenic NDV virus (Velogenic) Cloaca feces, Trachea
IBV Real-Time Detection Test Kit PD65-09-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Infectious Bronnchitis Virus Diarrheal faeces, Cloaca Feces, or Trachea
TRT Real-Time Detection Test Kit PD65-10-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Infectious Turkey Rhinotracheitis Nusalexudates,Choanalcleftswab,Scrapingofsinusandturbinatetissue
IBDV Real-Time Detection Test Kit PD65-11-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Infectious Bursal Disease Virus Bursa of Fabricious, Feces
REO Real-Time Detection Test Kit PD65-12-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Avian Reovirus Faeces, Trachea, Liver, Bursa, Kidney, Spleen
ORT Real-Time Detection Test Kit PD65-13-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Ornthobacterium rhinotracheale Swab (drag swab)
Industrial MYCO G/S Real-Time Detection Test Kit PD65-14-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Avian Mycoplasma gallisepticum, and M. iowae, M. meleagridis , M. synoviae Choanalcleft,Oropharynx,Oesophagus,Trachea,Eeyes,Cloaca,Phallus,Nalalcavity,Ifraorbitalsinus,Airsacs
Animal ALV Real-Time Detection Test Kit PD65-15-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Avian Leukosis Virus Liver, Spleen
AIV H5/N1 Duplex Real-Time Detection Test Kit PD65-51-(20) Real-Time PCR 96 (24) reactions/Kit Detection of AIV H5 and N1 Diarrheal faeces, Cloaca Feces, or Trachea
SIV A(H1N1) Real-Time Detection Kit PD64-01-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Swine Influenza Virus H1 typing Nasal fluid or Tracheal swab and Lung tissue
SIV Real-Time Detection Test Kit PD64-02-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Swine Influenza Virus Nasal fluid or Tracheal swab and Lung tissue
PCV-2 Real-Time Detection Kit PD64-03-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Procine Circovirus Tissue samples (Lung, Liver, Mesenteric lymph node)
PRRSV Real-Time Detection Kit PD64-04-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Procine Reproductive and Respiratory Syndrome Virus Blood, Serum, Tissues, Swab, Semen
CSFV Real-Time Detection Kit PD64-05-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Classical Swine Fever Virus Blood, Serum, Viral culture, Tissues
InfluenzaA(H1N1)Real-TimeDetectionTestKit(2Wellstype) PD65-18-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Novel Influenza A(H1N1) Virus (MP-House keeping gene/H1) Nasal fluid or Tracheel swab and Lung tissue
InfluenzaA(H1N1)Real-TimeDetectionTestKit(4Wellstype) PD65-19-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Novel Influenza A(H1N1) Virus (MP/NP/H1/House Keeping gene) Nasal fluid or Tracheel swab and Lung tissue
FMD Real-Time Detection Kit PD68-01-(24) Real-Time PCR 96 (24) reactions/Kit Detection of Foot and Mouth disease Virus Oral mucosa and Tongue swabs, Vesicular epithelium
BVDV Real-Time Detection Kit PD63-02-(24) Real-Time PCR 96 (24) reactions/Kit Detection of Bovine Viral Diarrheal Disease Virus Blood, Serum, Tissues (Lung, Spleen, etc.), Milk, Ear tag
BTV Real-Time Detection Kit PD63-03-(24) Real-Time PCR 96 (24) reactions/Kit Detection of Bluetongue Virus Blood
IBR Real-Time Detection Kit PD63-04-(24) Real-Time PCR 96 (24) reactions/Kit Detection of Infectious Bovine Rhinotracheitis Virus Semen, Nasal swabs, Conjunctival and Vaginal swabs
SBV Real-Time Detection Kit PD63-06 Real-Time PCR 96 (24) reactions/Kit Detection of Schmallenberg Virus Tissues(brain, spleen), Blood, Serum
PET
CHW Real-Time Detection Kit PD61-02-(20) Real-Time PCR 96 (24) reactions/Kit Detection of Canine Heartworm Whole blood, Plasma or Serum
Animal

OneStep RT-PCR Kit RK50-01 RT-PCR 100 Reactions
SYBR Green RT-PCR Kit RK50-02 RT-PCR 1000 Reactions
Viral RNA purification Kit RK50-90 250 Reactions
Global expertise in In Vitro Diagnostics
CIA 13-04E

2013 Livestock Catalogue-20150318-121800

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IIPCR Kit for Avian disease

Avian Influenza type A virus antigen

Spleen Small Intestine

Avian Influenza type A subtype 5 virus antigen

1 Collect swap sample from trachea, kidney, cloaca or feces. C T S

1 Serum 2 1 drop of serum 3 3 drops of assay diluent

1 Collect swap sample from oropharynx, kidney or cloaca. C T S

3 Take the supernatant with disposable

1 Collect swap sample from trachea, kidney or cloaca (feces). C T S

the presence of distinctive lesions in the Bursa of Fabricious and accompanying

with disposable dropper.

Avian Influenza type A virus antibody

3. Add 50㎕ of anti AIV antibody-HRP to each wells 3 days

4. Incubate plate for 30 minutes at 37℃ 1.2

5. Wash plate 6 times 1

for 10 minutes at room temperature

9. PI value=[1-(OD sample/mean OD negative)] x 100 0

5. Wash plate 6 times 100

6. Add 100㎕ of substrate solution and incubated for 15 minutes 80

7. Add 100㎕ of stop solution

8. Measure the optical density (OD) at 450 nm with reference 20

9. PI value= [1-(OD sample/mean OD negative)] x 100

6. Add 100㎕ of enzyme conjugate and incubate for 30 7

minutes at room temperature 6

7. Wash the wells 5 times 5

8. Add 100㎕ of substrate solution to each well 4

positive- mean OD negative] x 100

6. Add 100㎕ of enzyme conjugate to each well 20

7. Incubate the wells at 18~25℃(Room Temperature) for 30 0

8. Add 100㎕ of substrate solution to each well and Incubate

10. Read the absorbance of the wells with a spectrophotometer

11. S/P value=[OD samples - OD of negative control]/[OD 1

positive control – OD of negative control] 0.5

List of PCR Kit for Avian disease

Real-time IBV Real-Time Detection Kit Infectious Bronchitis Virus PD65-09

IIPCR Kit for Swine disease

Good Too much

3 Wait 30 seconds for sedimentation and

Good Too much

3 Wait 30 seconds for sedimentation and

• World's first commercialized rapid test kit for detection of

Good Too much

3 Wait 30 seconds for sedimentation and

Sensitivity and specificity study

1.5 AniGen 1.5 AniGen

0.5 cut off 0.5 cut off

Compare with other commercial ELISA

AniGen PRRS Ab ELISA Kit is more sensitive than others

Days post vaccination

“I” Commercial vol.2

<PRRS vaccination DPI results>

• Easy sample collection • Reading Time : 1 hour and 45 minutes

each well 10.0

4. Incubate the plate at 37±1℃ for 60 minutes 8.0

6. Dispense 100㎕ of diluted enzyme conjugate to each well 2.0 IgG

7. Incubate the wells for 30 minutes at37±1℃ 0.0

11. Dispense 100㎕ of stopping solution

12. Measure the optical density (OD) at 450nm with reference 60

4. Incubate plate for 30 minutes at room temperature

8. Wash plate 5 times 0 7 14

Days post infection

wavelength at 620nm 8.0