QUICK GUIDE

2021-0010 GSP®

Quick guide for

GSP Instrument

Firmware version 1.4

2021-9220-04
May 2015

Quick guide for typical

workflow with GSP The GSP® instrument is a fully
automated, high throughput analyzer
instrument for time-resolved and prompt
fluorescence analysis of samples in
microtitration plates. It is intended for
in vitro quantitative / qualitative
determination of analytes in body fluids. The GSP instrument and GSP
chemistries are for professional use only.
This guide is intended to be a supplement to the User Manual and presents
the daily workflow key steps in a condensed format.

Note! Operators should be trained in the operation of GSP and

should be familiar with the contents of the relevant user
instructions supplied with the product.

Manufacturer: Wallac Oy, Mustionkatu 6, FI-20750 Turku, Finland.

Tel: 358-2-2678 111. Fax: 358-2-2678 357. Website: www.perkinelmer.com
1. Log on
4. Empty waste
Note that the Instrument will automatically empty the
internal waste bottle if connected to a drain.

The default PIN for GSP Operator and GSP Supervisor

is “2021”. Type in the PIN and press OK. You may have
your own login name and password.
2. Run washer test Empty the internal waste bottle by pressing Start.
Also remember to check if the external waste bottle needs
In the Maintenance screen: Press Start if you need to to be emptied.
run the washer test.
5. Punch the assay plates
Remember that plate
information must be
available in the
workstation. You can
Go back to the Home screen to see if there is enough enter it manually
water in the instrument. using Plate Generator.
Note that the instrument can be configured to run the
washer test automatically.
3. Fill water 6. Add reagents
Note that the instrument will automatically fill the Assay reagents
internal water bottle if connected to a water source.
Check and remove any
bubbles on the surface or
inside the bottle and
vials. Load assay reagents
into the reagent cassette
so that the barcodes are
visible.
Put anti-evaporation caps
on the small vials.

Tip and waste cassettes

High Volume and Low
Volume tip cassettes must be
full of tips and the Waste tip
Fill the internal water bottle by pressing Start. Also cassette must be empty.
remember to check if the external water bottle needs Cassettes must be identified
to be filled. by means of a barcode label.
Reserve loading time 7. Load plates
Reserve time for
reagent loading
in the Reagent
and Tips screen.
Press Short to
reserve 10-
minute access
or press Long to
reserve a 20-
minute access
Barcodes facing outwards (towards you)
Reagent carousel
8. Add consumables if some are
missing
Check the areas
highlighted in red
on the Home screen.

9. Wait for schedule

To refresh the reagent inventory, press the above
button after loading and unloading. Loading is successful if a
schedule appears on the
After loading all cassettes, close the hatch and the user interface for the
door. plates loaded.
Bulk reagents

Add the DELFIA

Inducer bottles.
Read the
barcode and
load the bottle.
Note! Load full
bottles only.
10. Unload plates

Wash concentrate After plates

have finished,
Add Wash Concentrate at unload them.
any time. Select the type
of plates you
want to unload.
You can pre-
select the type
of plates to be
automatically
unloaded.
11. Batch and calculate measured
plates

or by comparing to the reference curve.

Click Calculate 14. Check control levels

Combine plates and click Calculate

12. Check instrument and workstation
flags The red outlined data points are from this particular
assay.
See next pages for flag descriptions.
15. Accept assay
13. Check the calibration curve

16. Export and print results

By comparing responses to QC certificate values

Open the assay and choose File – View Assay Export…

and Print Assay…
Instructions for handling flags in GSP

GSP INSTRUMENT FLAGS (with Severity level “Held” or “Prevented”)

1)
Flags Scope Retest
Disk remover failed to remove sample disk from the well. W YES
Uncertain wash result. This well is flagged because the disk remover failed to remove a sample disk. The disk
was found in another well but it could have come from this well. This means the disk may have affected the W YES
washing of this well.
Floating disk. Measured signal was {0} counts W YES
2)
Elution failed. The measured absorbance was {0} W YES
Abnormal dose. The {0} has detected an abnormal impulse when dispensing from the {1} W YES
Aspirated air. The {0} was above the liquid surface of the {1} when aspirating and has aspirated air. YES
Aspiration failed. The {0} hit the bottom of the {1}. YES
Dose too small. The {0} has detected a too low impulse when dispensing from the {1} W YES
The pipetting of the well immediately before or after this well has failed W YES
The variation of the pressure impulse during dispensing was too large. This may indicate that there is air in
W YES
the bulk dispenser
The pressure impulse during dispensing was too small. This may indicate that there is air in the bulk
W YES
dispenser
3)
The heated incubator temperature went outside the allowed temperature range Y/N
4)
The temperature of one or more of the shakers has been outside the allowed temperature interval Y/N
4)
The temperature of the Reagent Storage has been outside the limits Y/N
The assay failed for an unspecified reason YES
Large variations in dispensing pressure YES
Disk remover head was clogged during disk removing YES
Proper vacuum level was not present during disk removing. This may indicate that the tubing is clogged or
W YES
the valve did not function
4)
The plate is running late x after operation y Y/N
4)
Shaking failure. The shaker did not run as smooth as expected Y/N
4)
The internal temperature did not stay within the limits Y/N
4)
The humidity in the plate storage is x%, which exceeds the limit y%. Y/N
The instrument has detected a slip of x seconds in the internal clock. This might have affected the plate 4)
Y/N
processing
The previous washer test failed, the results might be affected. YES
The previous measurement module test failed. The results might be affected. YES
4)
It has been too long since the previous measurement module test. The results might be affected. Y/N
4)
It has been too long since the previous washer test. The results might be affected. Y/N
The previous HV pipette test failed, the results might be affected. YES
4)
It has been too long since the previous HV pipette test. The results might be affected. Y/N
The previous LV pipette test failed, the results might be affected. YES
4)
It has been too long since the previous LV pipette test. The results might be affected. Y/N
4)
It has been too long since the instrument disinfection was run. Results may be affected. Y/N
4)
Preventive maintenance is overdue. Results may be affected Y/N
GSP WORKSTATION FLAGS
Flag name Description Severity Retest
Expired QC Material System flag to notify user that QC Material has expired Held YES
Kitlot Expired System flag to notify user that a kit lot has expired Held YES
Expiration date not
Kit lot or QC material expiration date not defined Info NO
given
Information about GSP parameters that have to match for all plates in an
Condition Info NO
assay
Information provided by the GSP instrument for a plate, such as start
GSP plate log Info NO
time
Information provided by the GSP instrument about an error that has
Instrument Prevented YES
stopped plate measurement
Notifications provided by the GSP instrument related to single well See table
Instrument warning Notify
information and possible errors above
Curve Copied Flag informing which calibration is used for the plate Notify NO
6)
Bad Curve The calibration curve has more than one turning point Held YES
Amb? Concentration value cannot be resolved from the calibration curve Prevented YES
6)
No-Fit Calibration curve fitting failed Prevented YES
Calibration point value cannot be resolved from the fitted calibration 5)
No-Fit Prevented Y/N
curve
5)
HH The concentration is higher than the defined reporting limit Notify Y/N
5)
LL The concentration is lower than the defined reporting limit Notify Y/N
Set in QC 5)
QC Quality control rule violation Y/N
program
Sample codes are generated sequentially and there is no positive ID. This
Import warning Notify NO
flag appears when Plate Generator has been used to create sample data.
5)
Reject Calibration point is excluded from curve fitting Prevented Y/N
This is an automatically added flag that shows an override of result code 5)
ResultCode Edit Info Y/N
by the user
This is an automatically added flag that shows an override of Sample type
Sample changed Info NO
or level by the user
Pre-QC Flag assigned by the user to repeat sample before measurement Prevented YES
5)
Free Text Free text flag for the user to give Info Y/N

Footnote references
1) Single well flag is indicated with “W” in this column. If the flagged well is a control or calibrator sample you can consider
ignoring the result of the well provided that quality control efficiency will not degrade. If the flag is “Elution failed” please see
footnote 2.
2) See the package insert for assay specific instructions, all patient specimens need to be retested if T4 or BTD.
3) Retest only GALT and BTD plates.
4) Need for retest is determined by the control results.
5) Need for retest is determined by the laboratory supervisor.
6) Retest needed unless new calculation with an acceptable curve can be performed. If TGal plate, curve must not be copied
from another plate; but all patient samples must be retested.