IDENTIFICATION AND ANTIMICROBIAL RESISTANCE TO BACTERIA Escherichia

coli ISSOLATED FROM PLASTIC SHEET GUN INSEMINATION MADE IN BALI

COW THAT HAS REPEATED MARRIAGE IN CENTRAL LOMBOK KOPANG
ABSTRACT
The province of West Nusa Tenggara (NTB) has been recognized as a major area for
cattle production under the One Million Cows Program(BSS) and Special Efforts for
Compulsory Pregnancy Cows (UPSUS SIWAB).Artificial Insemination (IB) is one of the efforts
made by the government to make the program a success. However, there are several factors that
cause IB failure namely the presence of bacteria Escherichia coliin the reproductive tract of
female cows and if it is not controlled in giving antibiotics it will cause problems with bacterial
resistance to antibiotics. The purpose of this research is to isolate and determine
resistanceEscherichia coliTetracycline, Gentamicin and Penicillin G antibiotics. This research
was conducted in March 2021, sampling was carried out in Kopang, Central Lombok, while
sample examination was carried out at the Public Health and Calibration Laboratory of West
Nusa Tenggara Province. This study used six Balinese cows that experience repeated mating.
The sample in the form of uterine fluid was found through the Artificial Insemination plastic
sheet gun. A sterile plastic sheet is inserted into the vagina and then across the cervix to the body
or horns of the uterus. The ends of the plastic sheets were cut and put into (BHI) Brain Heart
Infusion and incubated for 24 hours. The sample was then implanted in Blood Agardan
incubated for 24 hours at 37˚C in the incubator. Bacteria that grow will be stained with Gram
stain and characterized by biochemical tests and analyzed based on Bergey's manual of
determinative bacteriology (Holt et al, 1994). Furthermore, resistance testing was carried out
using antibiotics, namely Tetracycline, Gentamicin, Penicillin G. The results showedwith the
disc diffusion method is 0 mm or no inhibition zone is formed.
keywords: Escherichia coli, Resistant, Tetracycline, Gentamicin, Penicillin G.