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Biotechnology and Its Application
Biotechnology and Its Application
Introduction
Eaten by insect
Death of insect
Pest Resistant plants
› Bt toxin is not toxic to all pest or pest become resistant against Bt
toxin
› Another approach to develop pest resistant crop against nematode
parasite- RNA interference (RNAi)
› Eg. Meloidegyne incognita- roots of tobacco
› Principle of RNAi to develop pest resistance- ‘Silencing translation
of specific mRNA’
› RNAi takes place in all eukaryotic organism as a method of cell
defense
› Silencing of specific mRNA due to complementary dsRNA (a type of
RNA molecule to enhance or inhibit translation of mRNA)- binds to
mRNA & prevent translation
› Source of dsRNA/ complementary RNA- Virus (RNA) or
Transposons- genetic element which replicate via an RNA
intermediate
Method to genetically modify crop through RNAi