Total Inorganic Phosphate

DR 2800 Analytical Procedure AP-5/2800

SPECIFICATIONS
Method: Spectrophotometric (DR 2800)
Range: 0.1 to 5.0 mg/L as PO4
Testing Time: 20 minutes

DESCRIPTION
The Total Inorganic Phosphate DR 2800 Procedure
was designed to help control inorganic phosphate
levels in most boilers and cooling water systems
without having to submit samples for laboratory
analysis. Inorganic phosphate-based products are
used to help control both scaling and corrosion in
boilers and cooling water systems. It is important
to monitor phosphate levels to ensure that the
proper level and type of phosphate are maintained
in the system. The test does not measure organic
phosphate. Nalco DR 2800 Spectrophotometer

ORDERING INFORMATION
Order all replacement parts and reagents by their Description Part No.
part numbers. To be sure this product is compatible 25 mL glass graduated cylinder
with your treatment program, contact your local with plastic cap 500-P1204.88
Nalco Sales Engineer. To place your order, please 125 mL glass Erlenmeyer flask
contact your local customer service department. (2 required) 500-P1503.88
3-1/2", 325 watt round hot plate
(2 required) 500-P1813.88
REPLACEMENT PARTS AND REAGENTS Teflon boiling chips, 2 oz
Required Parts (60 mL) bottle 500-P1725.88
5 mL serological pipet 500-P2171.88
Description Part No. 10 mL Pi-Pump Pipetter 500-P2721.88
Nalco DR 2800 Spectrophotometer 6017050
Reagent set, Ortho and Total
Inorganic Phosphate 460-P3109.88
Nalco Pressure Filter
(with filter membranes 420-C0091.88
Accessories INTERFERENCES
Description Part No. The species listed below do not interfere under the
Sample Cell, 10-mL, 1-inch glass stated values.
square matched pair 500-P2500.88
Sample Cell, 25-mL, 1-inch glass Species mg/L
square matched pair 500-P2502.88 Fe3+ 150
Pour through cell assembly kit 500-P2525.88 Ba2+ 50
47 mm, Filter Membrane, Cl– 5000
0.45 micron, 100/pkg 500-P1405.88 Ca2+ 400
1 mL plastic dropper pipet Pb2+ 20
with bulb 500-P0015.88 Cu2+ 20
2 oz (60 mL) dropper bottle 500-P0359.88 COD 500
1:1 HCl, 1.7 oz/50 mL 460-S0726.71 Al3+ 20
1:1 HCl, 3.4 oz/100 mL* 460-S0726.72 Mg2+ 1000
1:1 HCl, 16.9 oz/500 mL 460-S0726.74 CrO42– 60
1:1 HCl, 34.8 oz/1 L 460-S0726.75
1:1 HCl, 1.06 gal/4 L 460-S0726.77
TP-1, 3.4 oz/100 mL* 460-SH357.72 SAFETY PRECAUTIONS
TP-1, 16.9 oz/500 mL 460-SH357.74 Refer to Material Safety Data Sheet (MSDS) for
TP-1, 34.8 oz/1 L 460-SH357.75 specific reagent personal safety and disposal
XP-2, 0.9 oz/25 g 460-S5091.80 information.
XP-2, 3.5 oz/100 g 460-S5091.82
XP-2, 8.8 oz/250 g 460-S5091.83
DI Water, 1.06 gal/4 L 460-S0470.77 SUPPORT
Pressure Filter Unit Only 500-C0092.88 If you have any questions regarding this procedure,
47 mm glass fiber filter pad please contact your Nalco Sales Engineer. In North
(100/pkg) 500-P1413.88 America you can also contact the Nalco Equipment
47 mm, 0.45 micron filter Solutions Help Desk at 1-800-323-8483.
membrane (100/pkg) 500-P1405.88
4 oz. (120 mL) plastic bottle
with 0.5–1.0 mL pipet 500-P0346.88
0.5–1.0 mL pipet, standard size 500-P2120.88
0.5–1.0 mL pipet/cap/bulb
assembly 500-P2179.88
Blue pipet bulb for 0.5–1.0 mL pipet 500-P2403.88
Orange pipet bulb for 1 mL plastic
dropper pipet 500-P2404.88
1 oz (30 mL) vial with
0.5 gram scoop 500-P0350.88
0.1 and 0.5 gram scoops
(3 of each size) 500-P0354A.88
Dial thermometer, 50 to 300°F 500-P2019.88
Digital thermometer, –40 to 450°F 500-P2098.88
Lab thermometer, –10 to 260°C,
glass 500-P2020.88
Lab thermometer, 0 to 230°F, glass 500-P2028.88
TOTAL INORGANIC PHOSPHATE DR 2800 PROCEDURE
Special Note: This test does not measure organic phosphate.
Stored
Programs
1 2
Touch:
Stored Programs
Select stored program
738 PO4 Total Inorg.
Touch:
Start
Note: Phosphate levels
higher than 5 mg/L may be
determined by dilution with
distilled water.
4 5
Add 2 mL of TP-1
(460-SH357) to the other
flask (the prepared sample).
Swirl to mix.
Add 25 mL of filtered sample
into each of two 125-mL
Erlenmeyer flasks.
Place both flasks on hot
plates and bring to a gentle
boil.
Touch the TIMER icon to
begin the 5-minute boiling
period.
Notes: Other sample sizes
can be used depending on
the expected phosphate
concentration in the sample.
All dilutions should be made
with deionized water.
Sample should be com-
pletely free of haze. To
improve filtration of highly
turbid sample, filter under 3
pressure through glass
fiber pad on top of the
0.45 micron filter Add 2 mL of 1:1 HCI to one
membrane. Do not use a of the flasks (the blank).
glass fiber pad alone. Swirl to mix.
Rinse all glassware with
1:1 HCI (460-S0726). Note: To avoid contamin-
ating and deteriorating the
reagents when adding test
reagents, be careful that the
tips of the droppers or
pipets do not touch the
flasks or solutions within.
Note: It is important that
the sample be boiled
gently. Adjust the hot plates
to a setting that permits a
gentle boil. Adhering to a
boiling time of 5 minutes
will provide consistent
results. A small loss of
water may occur due to
evaporation. Use a watch
glass to cover the top of 6
the flasks to prevent
evaporation. Teflon® Boiling When the timer beeps,
Chips (500-P1725.88) will remove both flasks from their
promote smooth boiling. hot plates.
Touch the TIMER icon to
begin the 5-minute cooling
period.
 7 8 9 10
When the timer beeps, Using a serological pipet, Add 14 drops of freshly Touch the timer icon.
transfer the contents of each add deionized water to each made XP-2 (S5091) to each The ten-minute reaction
flask to a 25-mL sample cell. cell to bring the volume to of the cells and swirl to mix. period will begin.
the 25 mL mark. Then add
an additional 2 mL of
deionized water to each cell.

Zero Read

11 12 13 14
When the timer beeps, place Touch ZERO. Place the sample cell Touch READ
the cell containing the blank containing the prepared
solution into the cell holder. The display will show: sample into the cell holder. The results in mg/L PO4 will
3– be displayed.
0.0 mg/L PO4

Notes: For best results, XP-2

solution should be prepared
fresh daily. Add one powder
pillow of XP-2 or one heap-
ing 0.5 gram scoop (blue
scoop) of XP-2 (460-S5091)
to a 2-oz dropper bottle and
fill to the shoulder with
deionized water. Cap and
mix thoroughly.
At room temperature, the
full ten-minute color
development time is
required. The color is stable
for at least ten minutes after
full color development.

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