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  • Washed RBC Preparation

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    farailaika ni ayu auddeta
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    The document provides steps to prepare washed red blood cells through centrifugation and washing. The whole blood is centrifuged to separate plasma and buffy coat from red blood cells. The red cells are then resuspended and washed twice more with saline by centrifugation and removal of supernatant, and finally frozen for storage and shipment.

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    The document provides steps to prepare washed red blood cells through centrifugation and washing. The whole blood is centrifuged to separate plasma and buffy coat from red blood cells. The red cells are then resuspended and washed twice more with saline by centrifugation and removal of supernatant, and finally frozen for storage and shipment.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    16 views1 page

    Washed RBC Preparation

    Uploaded by

    farailaika ni ayu auddeta
    The document provides steps to prepare washed red blood cells through centrifugation and washing. The whole blood is centrifuged to separate plasma and buffy coat from red blood cells. The red cells are then resuspended and washed twice more with saline by centrifugation and removal of supernatant, and finally frozen for storage and shipment.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    Washed Red Blood Cell Preparation

    http://geneticslab.emory.edu

    Step 1: Centrifuge the whole blood at 3000rpm (1800rcf) for 5 minutes

    Step 2: Remove plasma and buffy coat layer.

    Step 3: Resuspend the red cells in normal saline (0.9% NaCl) with approximately 2 times the
    volume of the red cells, and invert the tube to mix.

    Step 4: Centrifuge for 5 minutes at 2000 rpm and discard the supernatant.

    Step 5: Repeat Steps 3 & 4 twice for a total of 3 washes or until the supernatant is clear

    Step 6: Completely remove the supernatant and freeze the red cells at -20˚C, preferably at -80˚C.
    Ship frozen.

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