LEMBAR PERHITUNGAN

A. Perhitungan Kuantitas Terhadap Variabel Nutrisi

• Densitas
(𝑀𝑝𝑖𝑘𝑛𝑜+𝑎𝑞)−(𝑀𝑝𝑖𝑘𝑛𝑜)
Rumus: 𝑉 𝑝𝑖𝑘𝑛𝑜 = 𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡
(𝑀𝑝𝑖𝑘𝑛𝑜+𝑠)−(𝑀𝑝𝑖𝑘𝑛𝑜)
𝜌= 𝑉 𝑝𝑖𝑘𝑛𝑜

1. Variabel 1 (Nutrisi 2%, pH 7, Suhu 17 oC)

t0 t1 t2 t3
𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml 𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml 𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml 𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml
Mpikno = 32,95 gram Mpikno = 32,95 gram Mpikno = 32,95 gram Mpikno = 32,95 gram
Mpikno + aq = 59,49 gram Mpikno + aq = 59,49 gram Mpikno + aq = 59,49 gram Mpikno + aq = 59,49 gram
Mpikno + s = 58,05 gram Mpikno + s = 58,34 gram Mpikno + s = 58,55 gram Mpikno + s = 58,72 gram
(59,49)−(32,95) (59,49)−(32,95) (59,49)−(32,95) (59,49)−(32,95)
𝑉 𝑝𝑖𝑘𝑛𝑜 = 𝑉 𝑝𝑖𝑘𝑛𝑜 = 𝑉 𝑝𝑖𝑘𝑛𝑜 = 𝑉 𝑝𝑖𝑘𝑛𝑜 =
0,996 0,996 0,996 0,996

𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml 𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml 𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml 𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml

(58,05)−(32,95) (58,34)−(32,95) (58,55)−(32,95) (58,72)−(32,95)
𝜌= 𝜌 = 𝜌 = 𝜌 =
26,646 26,646 26,646 26,646

𝜌 = 0,942 gr/ml 𝜌 = 0,953 gr/ml 𝜌 = 0,961 gr/ml 𝜌 = 0,967 gr/ml

C-1
2. Variabel 2 (Nutrisi 4%, pH 7, Suhu 37 oC)
t0 t1 t2 t3
𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml 𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml 𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml 𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml
Mpikno = 32,95 gram Mpikno = 32,95 gram Mpikno = 32,95 gram Mpikno = 32,95 gram
Mpikno + aq = 59,49 gram Mpikno + aq = 59,49 gram Mpikno + aq = 59,49 gram Mpikno + aq = 59,49 gram
Mpikno + s = 58,08 gram Mpikno + s = 58,42 gram Mpikno + s = 58,60 gram Mpikno + s = 58,80 gram
(59,49)−(32,95) (59,49)−(32,95) (59,49)−(32,95) (59,49)−(32,95)
𝑉 𝑝𝑖𝑘𝑛𝑜 = 𝑉 𝑝𝑖𝑘𝑛𝑜 = 𝑉 𝑝𝑖𝑘𝑛𝑜 = 𝑉 𝑝𝑖𝑘𝑛𝑜 =
0,996 0,996 0,996 0,996

𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml 𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml 𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml 𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml

(58,08)−(32,95) (58,42)−(32,95) (58,60)−(32,95) (58,72)−(32,95)
𝜌= 𝜌 = 𝜌 = 𝜌 =
26,646 26,646 26,646 26,646

𝜌 = 0,943 gr/ml 𝜌 = 0,956 gr/ml 𝜌 = 0,963 gr/ml 𝜌 = 0,970 gr/ml

3. Variabel 3 (Nutrisi 6%, pH 7, Suhu 30 oC)

t0 t1 t2 t3
𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml 𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml 𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml 𝜌 𝑎𝑞𝑢𝑎𝑑𝑒𝑠𝑡 = 0,996 gr/ml
Mpikno = 32,95 gram Mpikno = 32,95 gram Mpikno = 32,95 gram Mpikno = 32,95 gram
Mpikno + aq = 59,49 gram Mpikno + aq = 59,49 gram Mpikno + aq = 59,49 gram Mpikno + aq = 59,49 gram
Mpikno + s = 58,1 gram Mpikno + s = 58,55 gram Mpikno + s = 58,82 gram Mpikno + s = 59,15 gram
(59,49)−(32,95) (59,49)−(32,95) (59,49)−(32,95) (59,49)−(32,95)
𝑉 𝑝𝑖𝑘𝑛𝑜 = 𝑉 𝑝𝑖𝑘𝑛𝑜 = 𝑉 𝑝𝑖𝑘𝑛𝑜 = 𝑉 𝑝𝑖𝑘𝑛𝑜 =
0,996 0,996 0,996 0,996

𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml 𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml 𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml 𝑉 𝑝𝑖𝑘𝑛𝑜 = 26,646 ml

(58,1)−(32,95) (58,55)−(32,95) (58,82)−(32,95) (59,16)−(32,95)
𝜌= 26,646
𝜌 = 26,646
𝜌 = 26,646
𝜌 = 26,646

C-2
 𝜌 = 0,944 gr/ml 𝜌 = 0,961 gr/ml 𝜌 = 0,971 gr/ml 𝜌 = 0,983 gr/ml

• %S
𝑉 𝑡𝑜𝑡𝑎𝑙 𝑉 𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
(𝐹−𝑀)( )( )×0,0025×100%
𝑉 𝑡𝑖𝑡𝑟𝑎𝑠𝑖 𝑉 𝑦𝑎𝑛𝑔 𝑑𝑖𝑎𝑚𝑏𝑖𝑙
Rumus: %𝑆 = 𝑉 𝑡𝑜𝑡𝑎𝑙 ×𝜌

1. Variabel 1 (Nutrisi 2%, pH 7, Suhu 17 oC)

t0 t1 t2 t3
F = 29 ml F = 31 ml F = 32 ml F = 30 ml
M = 20 ml M = 25 ml M = 28,4 ml M = 27,4 ml
V total = 237 ml V total = 237 ml V total = 237 ml V total = 237 ml
V titrasi = 5 ml V titrasi = 5 ml V titrasi = 5 ml V titrasi = 5 ml
V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml
V yang diambil = 5 ml V yang diambil = 5 ml V yang diambil = 5 ml V yang diambil = 5 ml
𝜌 = 0,942 gr/ml 𝜌 = 0,953 gr/ml 𝜌 = 0,961 gr/ml 𝜌 = 0,967 gr/ml
237 100 237 100 237 100 237 100
(29−20)( )( )×0,0025×100% (31−25)( )( )×0,0025×100% (32−28,4)( )( )×0,0025×100% (30−27,4)( )( )×0,0025×100%
5 5 5 5
%𝑆 = 5 5
237 ×0,942
%𝑆 = %𝑆 = 5 5
237 ×0,961
%𝑆 =
237 ×0,953 237 ×0,967

%𝑆 = 9,554% %𝑆 = 6,297% %𝑆 = 3,747% %𝑆 = 2,688%

C-3
2. Variabel 2 (Nutrisi 4%, pH 7, Suhu 37 oC)
t0 t1 t2 t3
F = 29 ml F = 31 ml F = 32 ml F = 30 ml
M = 22,3 ml M = 27 ml M = 29 ml M = 28 ml
V total = 237 ml V total = 237 ml V total = 237 ml V total = 237 ml
V titrasi = 5 ml V titrasi = 5 ml V titrasi = 5 ml V titrasi = 5 ml
V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml
V yang diambil = 5 ml V yang diambil = 5 ml V yang diambil = 5 ml V yang diambil = 5 ml
𝜌 = 0,943 gr/ml 𝜌 = 0,956 gr/ml 𝜌 = 0,963 gr/ml 𝜌 = 0,970 gr/ml
237 100 237 100 237 100 237 100
(29−22,3)( )( )×0,0025×100% (31−27)( )( )×0,0025×100% (32−29)( )( )×0,0025×100% (30−28)( )( )×0,0025×100%
5 5 5 5
%𝑆 = 5 5
237 ×0,943
%𝑆 = %𝑆 = 5 5
237 ×0,963
%𝑆 =
237 ×0,956 237 ×0,970

%𝑆 = 7,104% %𝑆 = 4,185% %𝑆 = 3,116% %𝑆 = 2,062%

3. Variabel 3 (Nutrisi 6%, pH 7, Suhu 30 oC)

t0 t1 t2 t3
F = 29 ml F = 31 ml F = 32 ml F = 30 ml
M = 23 ml M = 28,5 ml M = 30 ml M = 29 ml
V total = 237 ml V total = 237 ml V total = 237 ml V total = 237 ml
V titrasi = 5 ml V titrasi = 5 ml V titrasi = 5 ml V titrasi = 5 ml
V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml
V yang diambil = 5 ml V yang diambil = 5 ml V yang diambil = 5 ml V yang diambil = 5 ml
𝜌 = 0,944 gr/ml 𝜌 = 0,961 gr/ml 𝜌 = 0,971 gr/ml 𝜌 = 0,983 gr/ml

C-4
 237 100 237 100 237 100 237 100
(29−23)( )( )×0,0025×100% (31−28,5)( )( )×0,0025×100% (32−30)( )( )×0,0025×100% (30−29)( )( )×0,0025×100%
5 5 5 5
%%𝑆 = 5 5
237 ×0,942
%𝑆 = %𝑆 = 5 5
237 ×0,961
%𝑆 =
237 ×0,953 237 ×0,967

%𝑆 = 6,357% %𝑆 = 2,602% %𝑆 = 2,060% %𝑆 = 1,017%

• %AL
𝑉 𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛
𝐴×𝐵×90×( )
𝑉 𝑦𝑎𝑛𝑔 𝑑𝑖𝑎𝑚𝑏𝑖𝑙
Rumus: %𝐴𝐿 = × 100%
𝐶 ×100

1. Variabel 1 (Nutrisi 2%, pH 7, Suhu 17 oC)

t0 t1 t2 t3
A = 0,1 ml A = 0,2 ml A = 0,3 ml A = 0,35 ml
B = 0,025 N B = 0,025 N B = 0,025 N B = 0,025 N
C = 10 ml C = 10 ml C = 10 ml C = 10 ml
V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml
V yang diambil = 10 ml V yang diambil = 10 ml V yang diambil = 10 ml V yang diambil = 10 ml
100 100 100 100
0,1×0,025×90×( ) 0,1×0,025×90×( ) 0,1×0,025×90×( ) 0,1×0,025×90×( )
5 5 5 5
%𝐴𝐿 = × 100% %𝐴𝐿 = × 100% %𝐴𝐿 = × 100% %𝐴𝐿 = × 100%
10 ×100 10 ×100 10 ×100 10 ×100

%𝐴𝐿 = 0,225% %𝐴𝐿 = 0,450% %𝐴𝐿 = 0,675% %𝐴𝐿 = 0,788%

2. Variabel 2 (Nutrisi 4%, pH 7, Suhu 37 oC)

t0 t1 t2 t3
A = 0,2 ml A = 0,4 ml A = 0,5 ml A = 0,6 ml
B = 0,025 N B = 0,025 N B = 0,025 N B = 0,025 N
C = 10 ml C = 10 ml C = 10 ml C = 10 ml

C-5
 V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml
V yang diambil = 10 ml V yang diambil = 10 ml V yang diambil = 10 ml V yang diambil = 10 ml
100 100 100 100
0,2×0,025×90×( ) 0,4×0,025×90×( ) 0,5×0,025×90×( ) 0,6×0,025×90×( )
5 5 5 5
%𝐴𝐿 = × 100% %𝐴𝐿 = × 100% %𝐴𝐿 = × 100% %𝐴𝐿 = × 100%
10 ×100 10 ×100 10 ×100 10 ×100

%𝐴𝐿 = 0,450% %𝐴𝐿 = 0,900% %𝐴𝐿 = 1,125% %𝐴𝐿 = 1,350%

3. Variabel 3 (Nutrisi 6%, pH 7, Suhu 30 oC)

t0 t1 t2 t3
A = 0,4 ml A = 0,5 ml A = 0,6 ml A = 0,55 ml
B = 0,025 N B = 0,025 N B = 0,025 N B = 0,025 N
C = 10 ml C = 10 ml C = 10 ml C = 10 ml
V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml V pengenceran = 100 ml
V yang diambil = 10 ml V yang diambil = 10 ml V yang diambil = 10 ml V yang diambil = 10 ml
100 100 100 100
0,4×0,025×90×( ) 0,5×0,025×90×( ) 0,6×0,025×90×( ) 0,55×0,025×90×( )
5 5 5 5
%𝐴𝐿 = × 100% %𝐴𝐿 = × 100% %𝐴𝐿 = × 100% %𝐴𝐿 = × 100%
10 ×100 10 ×100 10 ×100 10 ×100

%𝐴𝐿 = 0,900% %𝐴𝐿 = 1,125% %𝐴𝐿 = 1,350% %𝐴𝐿 = 1,238%

ρ (gram/ml) pH %S %AL
Variabel
to t1 t2 t3 to t1 t2 t3 to t1 t2 t3 to t1 t2 t3
1 0,942 0,953 0,961 0,967 6 5 4 3 9,554% 6,297% 3,747% 2,688% 0,225% 0,450% 0,675% 0,788%
2 0,943 0,956 0,963 0,970 5 4 3 3 7,104% 4,185% 3,116% 2,062% 0,450% 0,900% 1,125% 1,350%
3 0,944 0,961 0,971 0,983 5 4 3 2 6,357% 2,602% 2,060% 1,017% 0,900% 1,125% 1,350% 1,238%

C-6