Universidad Distrital Francisco José de Caldas

Genética molecular
Karen Lorena Gómez Tegua - 20182140099

BLASTn
1. Encontrar los organismos a los que pertenecen estas secuencias.

1. Samia ricini gb mRNA for globin, complete cds

2. Bombyx mori globina 1 (Glob1), variante de transcripción X4, ARNm

3. Octopus sinensis globina (LOC115213712), ARNm
4. Ictalurus punctatus subunidad de hemoglobina tipo alfa (LOC108257040), ARNm

5. Callorhinchus milii clone P04M02.Spleen.s08 adult beta-type globin mRNA, complete

cds
6. Petromyzon marinus globin (LOC116951551), ARNm

7. ARNm de globina de Caenorhabditis elegans, cds completos

2. Determinar la función de cada una.

1. Samia ricini gb ARNm para globina, cds completos.
2. Bombyx mori globina 1 (Glob1), variante de transcripción X4, ARNm.
3. Octopus sinensis globina (LOC115213712), ARNm.
4. Ictalurus punctatus subunidad de hemoglobina tipo alfa (LOC108257040),
ARNm.
5. Callorhinchus milii clon P04M02.Spleen.s08 globina de tipo beta adulta
RNm, cds completos.
6. Petromyzon marinus globin (LOC116951551), ARNm.
7. ARNm de globina de Caenorhabditis elegans, cds completos.

3. Reportar la longitud de la secuencia, porcentaje de identidad, query cover, así

como el E-value. 
1. Query cover 100% E-value. 0.0

2. Query cover 100% E-value. 0.0

3. Query cover 100% E-value. 0.0
4. Query cover 100% E-value. 0.0

5. Query cover 100% E-value. 0.0

6. Query cover 100% E-value. 0.0

7. Query cover 100% E-value. 0.0

4. Modificar los títulos en el archivo de texto, sin borrar el símbolo ">" que pertenece
al archivo FASTA.

1. >Samia ricini gb mRNA for globin, complete cds

ATGGGCAACTGGATAAGCCAGTTCTGGTGGGGCGGTGATCCGGATGAAGTGAATCCAATATCC
GGACTGA
CGAGAAGAGAAATATACGCCGTTCAGAAATCGTGGGCACCAGTATTTGCGAACTCAATACCCAA
CGGTGC
GGAGCTTTTAAGACGATTGTTTCAAACATTTCCCGAAACAAAAGAATTCTTCAAGATGATCCGCA
AACTG
CCTGACGAAGAATACATACAGAATCCTCAGTTTAGAGCGCACGTCATCAACTTAATGACTTCCCT
GAATC
TCGCAGTGAACAATCTTAACCAACCCGAGGTTGTGGCAGCGATGATGAACAAACTCGGAGAAT
CGCATAA
GCGCAGGCAAATTAAAGAAAGACATTTCGGTGATCTGAAACAGGTTATAGTGACAATGTTCATC
GAAGTA
CTACATCTAGACGGTGACACGCTGACGGCTTGGGACAAAACGGTCACGTTCTGGTACAAGCATA
TATTCG
AAACTCTCAACACGGCGGAAGAGTCCAGATAA

2. >Bombyx mori globina 1 (Glob1), variante de transcripción X4, ARNm

ATGGGCACCTGGTTTAGCTACATGTGGTGGGGTGGGGATCCGGACGTGGTGAACCCGGTCTCC
GGGCTCA
CGAGGAGAGAGATACACGCAGTTCAAAAATCATGGGCCCCCGTCAACGCCAACTCTTTTGCAAC
CGGCAG
TGAACTATTGAGAAGACTATTCAACACGTATCCTGACACCAAAGAATATTTCAAAATGGTCCGG
AAGCTT
CCCGAGGAGGAGTACTCGCAGAACCCGCAGTTCAAGGCGCACGTCATCAATTTAATGACGTCAT
TGAATT
TGGCCGTCAACAATCTGAATCAACCTGAGATAGTTGCTGCCATGATGACTAAACTTGGTGAATC
TCACAG
GAGGCGACAGATCAAGGAGAAGAATTTTCATGAGCTAAAAGAAGTTATAGTGAAACTGTTCAT
AGATGTA
CTACGTTTGGACGACGCCACACTGTCAGCGTGGGGGAAGACTGTCGAATTCTGGTACAAGCAT
ATATTTG
TCACATTGAATAGCCCGGAAGAGACAAGATAA
3. >Octopus sinensis globina (LOC115213712), ARNm
ATTCTTCGCAGATATATGAAAACAGTAAATGAATAAATAAAATATAGACATACACATCTTTCTCT
AAACG
TATATGCCTCAACTGGCAGACCAAAACTTTTAAATAAGATACTCCAATTTGTTGGAGTAATATTC
AAGAT
TGTGGATTCAATACTACAAAACTTTGGAATCATGCCTGCCAAAGATGAAGAAGATGTTGATCCT
GCTACT
GGATTGATGGTCAAAGAAAAAGCTGCTATCGTAGAAAGCTGGAAATATGTTTTACCAAACCAGA
AGGTCG
AATCTATAGCATTCTTCGAAAAGTTGTTCACAACATACCCACAATACATAGACTATTTCCCTAAGT
TCAA
AGGCAAACAATTGGCTGAAATGAAAGAAATGGGCAGTTTTGTTTTGCATGCAAAAAGTGTGTTC
ACAGCT
TTGACTGCCATTGTCAACAATCTTGATAATGCTGATTATCTAGTGAAGTTGCTGCTCAAAACAGG
CAAAG
ACCACAAAATGAGAGGGGTTCCGAAACAGGCGTTCCGTAACTTGGCTTTGGTGTTTATGGATTT
CATGGA
AGAGAGATTGGGAAGCAACTTCACTCCTTTGGCAAAATCTTCGTGGGAGAAAGCGTTAGCAGTT
GTGGTT
GATGTCTGCCATAAAGGTATGACTGATGAGTGATAAAAATGTTGCCTCAAAGACCATATTGTAC
CTTTTT
AGGTACAAGGACAAAAACAACAATAACAACAGGAAAAAA

4. >Ictalurus punctatus subunidad de hemoglobina tipo alfa (LOC108257040), ARNm

TTTTTTTTGAGGTGAAAAAACCGGATCAAGGCCGCAATGAGTCTCTCAGCTAAAGACAAAGCCG
TCGTCA
AAGACCTTTGGGCCAAGATCGCTCCGAAGCTGATAAAATTGGCGCCGAGGCACTTGGCAGGCT
GTTTGAG
GTCTACCCTCAGACAAAGACTTACTTCTCTCACTGGTCTGATCTTACTCCTGGATCAGCCCAAGT
GAAAA
AGCACGGAGGTGTAATTGTAAGGAAAATTGGAGAAGCCGTTGGCCACATTGACGATCTTACTG
GAGCTCT
AAGCTCACTAAGCGAGCTGCACGCTTTCAAGCTCCGTGTTGACCCTGTTAATTTCAAGCTCCTGT
CCCAC
ACCATCGAGGTGAGCATTGCCTTGTTCTTCCCCGCGGAGTTCACTCCTGAAGTTCATGTGTCTTT
TGACA
AGTTTCTTCAGAACCTGGCCCTTGCTCTGTCTGAGAAGTACCGCTAAATGAAGTAGCAGTTTCAG
AAGAC
ATCATTAAAATGACATCCATAAATGATCTTGACAATAAATATAATTGAACAATA
5. >Callorhinchus milii clone P04M02.Spleen.s08 adult beta-type globin mRNA, complete
cds
ACAGCTTTCTTCCAGCATAATCCTCATCAGGTTGAGCTTGCAAAAAAGAGGCAGAAAAATGGTT
CAGTGG
TCACAGGCAGAGCTTGATGTCATCCAAGGCAAATGGGCTGCTCTAGACCCAGAAAAGTTCGGT
GGCAAAG
CACTCGCCAGAATGTTTGTGGTCTACCCCTGGACCAAGAGATACTTCGGCAAATTCGGTGGCAG
GTTCAA
GGCCTCTGATCCGGTGGTCATGGAACATGGAGCCAAGGTGATGGGGAAAATGCAAGTTGCTGC
CAAAGAT
CCAGAAAAGATTAAGGAAATCTTTGAATACCTCAGCAAGCGTCACTCTGATACCATCCATGTGG
ATCCCG
AAAACTTCAAGCTCCTGGGTAGTTGCATGCTTGTTGAAATGGCTATGACCAAAGGAGACTGGTC
CCCGGA
AATCGAAGCAATCAACCGCAAATTCGTTGACGTCTCAATCGCTGCTCTGTCCAGAAAGTACCATT
AAATC
TGCGGAGAAAAGGGTGGCTCCGTTTTGTCTTATTTTGTTTCCTTTTTCGTTAACTCCGGAAAAGG
AAACA
AAAGGCAATAAGAACAACAATCGCCAGGGATCCATCTGAAACTAATGCAATTCTCTGTATGCTC
TTAAGA
AATTGGACGACAAGCAGGTTTTAAGACATGCGTGTAAAACACAATAAACTTTGATGAATTTCGA
AAACTA
TTGCTTCAGCTTTGTATGCAATCTTGCAACCCAATTTATTTCCGAGAGTGTCTCAAGGAAATATTC
CTGT
TGCTTACTACTTCAATAAAAAAAATCAATACTTTTG

6. >Petromyzon marinus globin (LOC116951551), ARNm

TCCTCAGATGAGTAGCTCATCACTTCAGACTCCCTTCGCAGGATCATCGCCGATCAACTCCAAAA
GCAAT
CGCCATGCCTATCGTTGACTCTGGAAGCGTTGCGCCTCTGAGCGCTGCTGAGAAGACCAAAATC
CGCAGC
GCGTGGGCCCCCGTGTACTCCAATTATGAGACTTCCGGTGTCGACATCCTGGTGAAGTTCTTCAC
CAGCA
CCCCCGCTGCTCAGGAATTTTTCCCCAAGTTCAAGGGGATGACTACGGCTGATCAGCTCAAGAA
ATCCGC
GGACGTGCGCTGGCACGCCGAGAGGATCATCAACGCCGTCAATGATGCCGTGACGTCCATGGA
TGACACC
GAGAAGATGAGCATGAAGCTAAGGGATCTGAGTGGCAAACACGCCAAAAGCTTCCAGGTGGA
CCCCCAGT
ACTTCAAGGTCCTGGCCGCGGTCATTGCTGACACCGTTGCCGCAGGCGATGCCGGCTTCGAGAA
GCTCAT
GAGCATGATTTGCATCCTGCTCAGGTCCGCCTACTAAACCAACGTGCACGGCCACTGCTGCAAG
ACTGCT
ACGCAACTGCCTTTGCCACATGATTTGCACCAGTTTCTGTTGACAGAGATAACAAATGTCTTGGA
GAATG
AAGCATCTTTCCGAGTTGTTCTTGTCAATGACAATAAAATGAATGTAACTTCCCAATGAAAATCA
ATAAA
ACTTCCATGCATCCAGA

7. >Caenorhabditis elegans globin mRNA, complete cds

TTTTTTTTTTGTTTGAGAGAAAAATGTCGATGAACCGTCAAGAAATTAGTGATCTCTGTGTGAAG
TCCCT
TGAAGGACGAATGGTTGGAACTGAGGCTCAAAACATTGAGAATGGAAACGCCTTCTACCGTTA
TTTCTTC
ACCAACTTCCCGGATCTTCGTGTCTATTTCAAGGGAGCCGAGAAGTACACTGCAGATGATGTGA
AGAAGA
GTGAGAGATTTGACAAACAAGGCCAACGTATTCTTCTTGCGTGCCATCTCCTTGCCAACGTCTAC
ACCAA
TGAAGAAGTCTTCAAGGGATACGTCCGTGAAACCATCAACCGTCATCGTATCTACAAAATGGAT
CCAGCT
CTGTGGATGGCATTCTTCACTGTATTCACTGGATATCTTGAATCTGTTGGATGTCTCAATGATCA
ACAAA
AGGCCGCATGGATGGCACTTGGAAAGGAATTCAATGCTGAGAGTCAGACACATCTCAAGAATT
CCAATCT
TCCACATGTTTAATAGATCTGAATGTTTTGAGCCTTTATATTGTATTTGAATGAGCTTTGAGTATT
ATAA
AAA

5. Una vez completada la información, realizar un alineamiento BLASTx para obtener

la secuencia de la proteína, escoger la secuencia que presente 100% de valor de
identidad y descargar la secuencia FASTA.

>Samia ricini gb mRNA for globin, complete cds

ATGGGCAACTGGATAAGCCAGTTCTGGTGGGGCGGTGATCCGGATGAAGTGAATCCAATATCC
GGACTGA
CGAGAAGAGAAATATACGCCGTTCAGAAATCGTGGGCACCAGTATTTGCGAACTCAATACCCAA
CGGTGC
GGAGCTTTTAAGACGATTGTTTCAAACATTTCCCGAAACAAAAGAATTCTTCAAGATGATCCGCA
AACTG
CCTGACGAAGAATACATACAGAATCCTCAGTTTAGAGCGCACGTCATCAACTTAATGACTTCCCT
GAATC
TCGCAGTGAACAATCTTAACCAACCCGAGGTTGTGGCAGCGATGATGAACAAACTCGGAGAAT
CGCATAA
GCGCAGGCAAATTAAAGAAAGACATTTCGGTGATCTGAAACAGGTTATAGTGACAATGTTCATC
GAAGTA
CTACATCTAGACGGTGACACGCTGACGGCTTGGGACAAAACGGTCACGTTCTGGTACAAGCATA
TATTCG
AAACTCTCAACACGGCGGAAGAGTCCAGATAA

FASTA
> BAG86629.1 globina [Samia ricini]
MGNWISQFWWGGDPDEVNPISGLTRREIYAVQKSWAPVFANSIPNGAELLRRLFQTFPETKEFFKMI
RKL
PDEEYIQNPQFRAHVINLMTSLNLAVNNLNQPEVVAAMMNKLGESHKRRQIKERHFGDLKQVIVTM
FIEV
LHLDGDTLTAWDKTVTFWYKHIFETLNTAEESR

6. Alineamiento Global (Global Align BLAST) https://blast.ncbi.nlm.nih.gov/Blast.cgi?

PAGE_TYPE=BlastSearch&PROG_DEF=blastn&BLAST_PROG_DEF=blastn&BLAST_SPEC=
GlobalAln&LINK_LOC=BlastHomeLink

1. Seleccionar 2 secuencias y alinear.

2. >Bombyx mori globina 1 (Glob1), variante de transcripción X4, ARNm

ATGGGCACCTGGTTTAGCTACATGTGGTGGGGTGGGGATCCGGACGTGGTGAACCCGGTCTCC
GGGCTCA
CGAGGAGAGAGATACACGCAGTTCAAAAATCATGGGCCCCCGTCAACGCCAACTCTTTTGCAAC
CGGCAG
TGAACTATTGAGAAGACTATTCAACACGTATCCTGACACCAAAGAATATTTCAAAATGGTCCGG
AAGCTT
CCCGAGGAGGAGTACTCGCAGAACCCGCAGTTCAAGGCGCACGTCATCAATTTAATGACGTCAT
TGAATT
TGGCCGTCAACAATCTGAATCAACCTGAGATAGTTGCTGCCATGATGACTAAACTTGGTGAATC
TCACAG
GAGGCGACAGATCAAGGAGAAGAATTTTCATGAGCTAAAAGAAGTTATAGTGAAACTGTTCAT
AGATGTA
CTACGTTTGGACGACGCCACACTGTCAGCGTGGGGGAAGACTGTCGAATTCTGGTACAAGCAT
ATATTTG
TCACATTGAATAGCCCGGAAGAGACAAGATAA

4. >Ictalurus punctatus subunidad de hemoglobina tipo alfa (LOC108257040), ARNm

TTTTTTTTGAGGTGAAAAAACCGGATCAAGGCCGCAATGAGTCTCTCAGCTAAAGACAAAGCCG
TCGTCA
AAGACCTTTGGGCCAAGATCGCTCCGAAGCTGATAAAATTGGCGCCGAGGCACTTGGCAGGCT
GTTTGAG
GTCTACCCTCAGACAAAGACTTACTTCTCTCACTGGTCTGATCTTACTCCTGGATCAGCCCAAGT
GAAAA
AGCACGGAGGTGTAATTGTAAGGAAAATTGGAGAAGCCGTTGGCCACATTGACGATCTTACTG
GAGCTCT
AAGCTCACTAAGCGAGCTGCACGCTTTCAAGCTCCGTGTTGACCCTGTTAATTTCAAGCTCCTGT
CCCAC
ACCATCGAGGTGAGCATTGCCTTGTTCTTCCCCGCGGAGTTCACTCCTGAAGTTCATGTGTCTTT
TGACA
AGTTTCTTCAGAACCTGGCCCTTGCTCTGTCTGAGAAGTACCGCTAAATGAAGTAGCAGTTTCAG
AAGAC
ATCATTAAAATGACATCCATAAATGATCTTGACAATAAATATAATTGAACAATA
2.  Reportar la longitud del query y del subject, porcentaje de identidad, positivos
(coincidencias) y porcentaje de gaps.

Longitud Query: 522

Subject: 544
Porcentaje de identidad positivos (coincidencias): 288/589(49%)
Porcentaje de gaps: 112/589(19%)