BIOLOGY vase
INVESTIGATORY
PROJECT:
STUDY OF THE EF#ECT OF
ANTIBIOTICS ON MICRO ORGANISMS
PRESENTED BY
R.SREENUPRIYA
XINeee
2obl344-2
CERTIFICATE
This is to certify that, this biology investigatory
project on the topic “STUDY OF THE EFFECT OF
ANTIBIOTICS ON MICRO ORGANISMS” has been
successfully completed by R.Sreenupriya of class
Xll-l under the guidance of Mr.Suman in partial
fulfilment of biology practical in the curriculum of
AISSCE-CBSE, New Delhi for the academic year
2019-2020.
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Signatudse e
xternal examiner _ Signature of internal examiner
—
Signature of principalACKNOWLEDGEMENT
I would like to thank CBSE and my school for
providing me with this wonderful opportunity. !
thank the biology department of the school
along with my sir Mr.Suman for helping me out
and lastly my friends and parents for supporting
and encguraging me.ABSTRACT
Antibiotics are the organic secretion produced by micro
— organisms, which in low concentrations are antagonistic to
the growth of other micro — organisms (mostly pathogens).
Antibiotics have proved very useful in combating several
bacterial diseases in man an animal.
Soil is a natural medium that harbours several types of micro
— organism. These micro- organisms can be frown on culture
media. The effect of different types of antibiotics can be
studied on the growth of micro - organism growing in culture
medium. This is an important subject, therefore the study of
effect of antibiotics on micro - organism has been taken for
the present project. :
An antibiotic can also be cl@sified according to the range of
pathogens against which it is effective. Penicillin G will
destroy only a few species of bacteria and is known as a
narrow spectrum antibiotic. Streptomycin is effective against
a wide-range of organisms and is known as broad spectrum
antibiotic.
ANTIBIOTIC RESISTANCE:
Antibiotic resistance is the development of bacterial
mechanisms that defeat the disruptive effects of antibiotics.
As a result, antibiotics that used to cure specific diseases by
preventing the growth of the associated bacteria no longer
work. Such drug resistance becomes common as more and
more bacteria change.Even when only a few of the bacteria are resistant to the
antibiotic used, the non-resistant bacteria are killed off while
the remaining ones multiply to continue causing the disease.
When this happens repeatedly, the resistant bacteria
become more common, and more cases of antibiotic failure
occur.
This is the situation at present. If the trend continues,
eventually most bacteria will be resistant, and antibiotics will
no longer be effective for disease control and prevention.
For example, several kinds of bacteria cause pneumonia, and
the type of antibiotic that breaks up bacterial DNA strands to
prevent the bacteria from splitting is often used to control
and cure the disease. For antibiotic resistant bacteria, these
antibiotics can no longer br@k up the DNA strands.
ZONE OF INHIBITION
The size of the zone of inhibition indicates the degree of
sensitivity of bacteria to a drug. In general, a bigger area of
bacteria-free media surrounding an antibiotic disk means the
bacteria are more sensitive to the drug the disk contains.
zone of
inhibition‘A population of bactoria
| grows, vulnerable to
NY) antibiotics.
Genetic mutations or gene
transfer make small num-
N bers of bactoria resistant
) to antibiotics.
Antibiotics kill the vulnera-
ble bacteria, leaving only
the resistant bacteria alive.
‘The resistant bacteria are
now able to grow rapidly.
Resistant bacteria transfer
their genes to new bacterial
cells, creating even more
antibaoterial resistance.HYPOTHESIS
PENICILLIN is a narrow spectrum bacterium, Therefore, the
chances of resistance of this antibiotic ayainst an unknown
bacterium is not much.
STREPTOMYCIN is used as a pesticide, to combat the growth
of bacteria beyond human applications. it is also a broad
spectrum of bacteria effective against both gram positive and
gram-negative bacteria. The resistance of this bacteria might
be more than that of the others.
AMPICILLIN is also a broad-spectrum bacterium and used ina
wide area. This has the ability to inhibit the bacteria better
than that of penicillin.
&EXPERIMENT
ai: This project aims to find out how the frequent use of antibiotics
affects bacteria resistance. This experiment is carried out using the