aor
ISMSO 14902 : 2001
Indian Standard
ANIMAL FEEDING STUFFS — DETERMINATION OF
TRYPSIN INHIBITOR ACTIVITY OF SOYA PRODUCTS
1 Scope
‘This Intemational Standard specifies @ method for the determination of the trypsin inhibitor activity (TIA) of soya
‘This trypsin inbbltor activity ls Indicative of the dagree of toasting ofthese products,
‘The dotection iit of the method is 0.8 malo.
2 Normative reference
The following normative document contains provisions which, trough reference in this tex, constitute provisions of
this International Stendard. For dated referanices, subsequer, amendments to, or revisions of, any of theta
‘publications do not apply. However, parties Ia agreements based on Ihis International Standard are encouraged to
Investigate the possbilty ot epplying the moal recent edition of the normative document indiceted below. For
Undated references, the latest edition of tha normative daaunent rsterred to applies. Members of ISO and IEC
tmeatah pain cery vol erator Sede
1S0 3696-1987, Water for analytical iaboretory vee — Speciication end test methods
3 Term and definition
For the purposes of this International Standard, the foflowing term and definition applies.
uM
{rypoin inhibitor activity eB
TA
‘m8s of vypein inhibited by the procedure doscribed In ths incematlonal Standard, divided by the mass ofthe test
same
NOTE The trypsin inhibir acy is expensed in miligrams per gram
4 Principle
Trypsin inhibtors are extracted from the samplo at pH 9,5.
Tes ekg bys ly nase ty ite ranvaarine peace F-AAAAY os etal The
‘quanthy of releesed p-ntroaniline is measured spectromISMSO 14902 : 2001
5 Reagents and materials
Use only reagents of recognized analyica! rade,
5.1. Water, complying with ot least grace 3 in accordance with ISO 3696.
5.2. Sodium hydroxide solution, (NAOH) = 0.01 mot
5.3 Hydrochlorle seld, HCI) = 6 molt,
54. Hydrochloric acid,