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aor ISMSO 14902 : 2001 Indian Standard ANIMAL FEEDING STUFFS — DETERMINATION OF TRYPSIN INHIBITOR ACTIVITY OF SOYA PRODUCTS 1 Scope ‘This Intemational Standard specifies @ method for the determination of the trypsin inhibitor activity (TIA) of soya ‘This trypsin inbbltor activity ls Indicative of the dagree of toasting ofthese products, ‘The dotection iit of the method is 0.8 malo. 2 Normative reference The following normative document contains provisions which, trough reference in this tex, constitute provisions of this International Stendard. For dated referanices, subsequer, amendments to, or revisions of, any of theta ‘publications do not apply. However, parties Ia agreements based on Ihis International Standard are encouraged to Investigate the possbilty ot epplying the moal recent edition of the normative document indiceted below. For Undated references, the latest edition of tha normative daaunent rsterred to applies. Members of ISO and IEC tmeatah pain cery vol erator Sede 1S0 3696-1987, Water for analytical iaboretory vee — Speciication end test methods 3 Term and definition For the purposes of this International Standard, the foflowing term and definition applies. uM {rypoin inhibitor activity eB TA ‘m8s of vypein inhibited by the procedure doscribed In ths incematlonal Standard, divided by the mass ofthe test same NOTE The trypsin inhibir acy is expensed in miligrams per gram 4 Principle Trypsin inhibtors are extracted from the samplo at pH 9,5. Tes ekg bys ly nase ty ite ranvaarine peace F-AAAAY os etal The ‘quanthy of releesed p-ntroaniline is measured spectrom ISMSO 14902 : 2001 5 Reagents and materials Use only reagents of recognized analyica! rade, 5.1. Water, complying with ot least grace 3 in accordance with ISO 3696. 5.2. Sodium hydroxide solution, (NAOH) = 0.01 mot 5.3 Hydrochlorle seld, HCI) = 6 molt, 54. Hydrochloric acid,

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