Dept of Laboratory Technology,

Bangladesh University of Health Sciences (BUSH)

Spectroscopy
1 What is electromagnetic radiations and describe the characteristics. Describe
Electromagnetic spectrum.
2 Mathematically Derive Beer’s and Lambert Law. OR proved that A=ε c l. What are the
factors depending on Molar absorptivity (ε)? Define the limitations of Beer-Lambert Law.
3 What is Molar absorptivity/molar absorption coefficient and Molar extinction coefficient?
4 What do man by Transition probability? Classify that with examples. Give the difference
between Allowed and Forbidden transition.
5 What are the restrictions for electronic transitions Or define the selection rules in the case of
electronic transitions.
6 What are the theory of Electronic spectroscopy? Define the types of electronic transition?
7 What is Chromophore? Give the examples. Classify.
What is Auxochrome? Give the examples. Classify.
8 What are the types of Absorption and intensity shifts? Define with examples.
9 What are the effect of Solvent Polarity on Absorption Wavelength in spectroscopy?
1 What are the effect of Degree of conjugation and Chemical structure in spectroscopy?
0
1 Woodward Fieser rules for calculating λmax in case of dienes. (Practice from given slide)
1
1 Compare and contrast between Colorimeter and Spectrophotometer.
2
1 Whit down the name of basic components of Spectrophotometer with diagram. And draw the
3 diagram how a spectrophotometer works.
1 What is Band Pass?? What is stray Light? Define.
4
1 What is advantages and disadvantages of Single beam and Double bam spectrophotometer?
5 Compare and contrast.
1 Write down the components and principle of Atomic Emission Spectroscopy or Flame
6 Photometry
1 Write down the components and principle of Atomic Absorption Spectroscopy (AAS)
7

Chromatography
1 What is the definition of chromatography? Classify the Chromatographic methods.
2 What art h Basic concepts of Chromatography method?
3 What are the classification of chromatography on the basis of separation mechanisms? Define
all.
4 Define principle, characteristic feature, advantages and application of
i) HPLC, ii) GC, iii) GPC and iv) TLC methods.
5 What art h Nature of stationary phase, Solid support and mobile phase of GC ad HPLC?
6 How can you Select the of appropriate detector in liquid /HPLC?
7 How can you Increase the HPLC Resolution?
Immunochemical techniques

1 What is antigen, Immunogen, Haptan? What are the properties? What are Monoclonal and
Polyclonal Ab? What are Affinity and Avidity?
2 What is the principles immunochemical analysis? Define the phases of Ag to Ab binding
proceeds. What Factors depends on the speed of immunochemical reactions?
3 Describe mechanism of Precipitin Reactions.
4 Describe Classical Quanitative precipitin curve with examples.
5 Define Ionic species, Ionic strength and polymer Effects of Precipitin Reactions.
6 Define and classify the Passive Gel Diffusion techniques. Define Double immunodiffusion/
Ouchterlony technique with examples.
7 Define Immunoelectrophoresis (IEP) technique with example.
8 Define Agglutination process with classification.
9 Compare and Contrast of Turbidi metric and Nephelo metric Assays
1 Define Competitive enzyme immunoassay.
0
1 Define Non-competitive enzyme immunoassay or two-site, or sandwich or ELISA assays.
1
1 What are the advantages using immunochemical methods and what are the analytical
2 problems in this techniques?

Chemiluminescence

1 What is Chemiluminescence? Write drawn reaction with example. Give applications.

2 What types of reaction are involved in Chemiluminescence? Define all.
3 How can you enhanced chemiluminescence technique?
4 Applications of Chemiluminescence.