pH Measurement and Buffer Preparation

pKa Weak Acid Conjugate Base

12.32 𝐻𝑃𝑂 𝑃𝑂

Using Henderson Hasselbalch Equation, determine log [(conjugate base)/(weak acid)]

𝑁𝑎 𝑃𝑂 𝑁𝑎 𝑃𝑂
𝑝𝐻 = 𝑝𝐾𝑎 + 𝑙𝑜𝑔 = 10 .
𝑁𝑎 𝐻𝑃𝑂 𝑁𝑎 𝐻𝑃𝑂

𝑁𝑎 𝑃𝑂 𝑁𝑎 𝑃𝑂
12 = 12.32 + 𝑙𝑜𝑔 = 0.4786300923
𝑁𝑎 𝐻𝑃𝑂 𝑁𝑎 𝐻𝑃𝑂

𝑁𝑎 𝑃𝑂
12 − 12.32 = 𝐴𝑛𝑡𝑖𝑙𝑜𝑔
𝑁𝑎 𝐻𝑃𝑂
Compute for the base and acid component using the equation M = B + A (where: M = Molarity of
Buffer Solution; B = Molarity of Base; A= Molarity of Acid)

Acid Component Base Component

M=B+A M=B+A
0.1 = 0.4786300923A + A B= M–A
0.1 = 1.4786300923A B = 0.1 – 0.06763016695
A = 0.06763016695 M B = 0.03236983305 M
Determine the actual grams of the weak acid and the conjugate base.
𝑤𝑒𝑖𝑔ℎ𝑡 𝑜𝑓 𝑠𝑜𝑙𝑢𝑡𝑒
𝑴𝒐𝒍𝒂𝒓𝒊𝒕𝒚 = 𝒎𝒐𝒍𝒆𝒔 /𝑳
𝑀𝑊 𝑜𝑓 𝑠𝑜𝑙𝑢𝑡𝑒

Grams of Weak Acid Grams of Conjugate Base

a) a)
0.06763016695 𝑥 0.03236983305 𝑥
= =
1𝐿 0.2 𝐿 1𝐿 0.2 𝐿

𝑥 = 0.06763016695 0.2 𝑥 = 0.03236983305 0.2

𝑥 = 0.0135260339 𝑚𝑜𝑙𝑒𝑠 𝑥 = 0.00647396661 𝑚𝑜𝑙𝑒𝑠

b) Moles x Molecular Weight = grams b) Moles x Molecular Weight = grams

268 𝑔 182 𝑔
0.0135260339 𝑚𝑜𝑙𝑒𝑠 𝑥 0.00647396661 𝑚𝑜𝑙𝑒𝑠 𝑥
𝑚𝑜𝑙 𝑚𝑜𝑙
= 3.624977085𝑔 𝑁𝑎 𝐻𝑃𝑂 = 1.178261923𝑔 𝑁𝑎 𝑃𝑂
≈ 3.62𝑔 𝑁𝑎 𝐻𝑃𝑂 ≈ 1.18𝑔 𝑁𝑎 𝑃𝑂