a — es ADSORPTION CHROMATOGRAPHY AIM, oe Seperate the wixture ef dyes by. wai adsorption chromate— and tp feann the -hechiques nthe Sepenaction forces. PRINCIPLE Adsorption chnomatsgsaphy. is based en adsorption ef Solute wolecules smte the Bonid va watrix Zuchas Ailecagel , alumina ete- In adsorption cleo wnactoeap sthene ane -two pes Forces, forces, attacking, Sollutes te° adsorbent C forces Se altvackion’) amd ones ‘ile ge HAemove Aolukes trem adsorbevit -to wove with the mokile pI Forces oF ATTRACTION a) dipole-dipole attyackion - Zance. takes place between polar adsorbent omd polan Solukes Gi), en_bonding ~Type ef bend wealcen than coValent bo-| nd Hiddrogen bode aft formed behseen tie ot! group Cas in Ailica)“and electron ebive. atews suchas O2,N Sn Soubes » cai) Weak covalent bouds - As those takes place. tuning Covnplex fo—| fumatien — ay Vanden waals tances - Neh polen attraction fponces ecu ‘Ge tween the abewms ef vuiclei amd alechenes of other akoms. FoRcES CAUSE SoLUTE MOVEMENTS Elution ~ It isthe tendenu, of solutes to dissolve amd mo- Ve with -the wobile phase The solvent used as mobile phase mu st be qust qeed enough te dissolve the solutes te allow comp-| zkition with the adsorptien power ef-the stabionan § phase.4¢.| ery aAhreng, Aolvents all used they will wash eut WW solutes together that Seperation. (i) “Displacement = Inthis case, Selveut molecules compete with lee ba asda on athe, adsorption Artes 2 “the skakionany, ghar “Teachers Signa— Experiment Name / Nos. Camiin [pao Doe TT jase. This competition makes olutes move in difrenent Specds- when a chalk is placed inside a containen with the so-} Went the solvent will vise uy due +e capillan, forces The compound that has skrongen intenactions wrth -the 4tation: {phase will move along slewe than 4 Compound -that has wea- len inkenackion withthe adsorbent ‘This leads 4a the sepena-| tien duets difference in mobility REQUIREMENTS: Phenol red selution, Methylene blue alkaline, Bromocresol green é 1] Solution, chalk piece, waken , Pebidish PROCEDURE W)| To 3m% of phenol ved Solutions wae thglene blue alkaline, Bromogr— esolqreen Golutions ane wixed in 4 petridish- Gi) Uahite chale was pled in-that pebidith by which sumple gat ul adsorbed. (iii)| Loaded chalk was placed ina petvidish with water as sdvenk Gv The mobile phase will ascend as 4 result of -the capillany ackien amd it will seach the sample. The components in-the sample mix- tune will be dissolved and carried by ascending Solveut - (),| The dyes in the mixbune qet adsorbed at varneus Levels and d 3 HWhus-they, can be sepencted OBSERVATION The digs in the mixtune - Blue, Red amd qreen qot adsorbed at various levels amd get seperated with blue colown ak the bo - »| Hom , Bed in-the middle end qpeen ak the top INFERENCE The dyes wene Sepenated te different tevels due tothe | f polan natune of the diye The same procedure cam be applied lyon proteins ten thein Seperation boy adsorption Chromatsencp— “Teachers Signatur‘Camiin [pan no [corer ieee “tes ULT Thus the given dye amixbune was sepenated using. adsorpti-| on chromategraphe Tete Sate