experi vent Name/ Nos Carlin [pra ai PRODUCTION OF INTRACELLULAR ENZYME INVERTAS E AIM | —+ 7 produce wrtvacellulan invertase euzyme. from yeast cells. THEORY ABOUT INVERTASE ‘ invertase enzume also known as p-pruchopenanoxidase euzy me} es Sucrose Cdisacchanide) in to ose amd pruckose invertase exhibits relatively, igh activity, efena broad pil va- f “ye ef 35-55 with optivnutle, Sean 4-5 theackivity rea ches jks maximum at 55°. A unik of enz activiky is de fin- ed as ameunt ef se Libenated penmi. ef enzyme Belud-ien pen minute unden Sstandarel assay, condition. The'specific ac sHivity, ef am enzyme isthe rumbSt ef. units ef activity per wrik mass Of protein - t MATERIALS REQUIRED WPS Medium qeast enctract - 1% Feptene -2% Aucnose ~ 20% PROCEDURE QJ 250 mt ef WPS medium was prepared and Sterilized. Gi)| Tothia sterile medium , 34 of Baker’s yeast pellet were added and incubated at 20°C at 120 pm : cadays Gii)| The culture was centrifuged at Ge0o yp _ fox 10 minute and the pellets obtaimed were dissolved in tom ef. citrake phosphate | bupeen ak pH) a5 Ay, of yeast: cells pen mbC ig mt) ——_ Gd The celt suspewsion of lomboblained fromabeve procedure, { was clvided inte 2 equal pants oF ml dnd was used toy eel disruption studies The-first pant of mi was Subjected te 4omd, hemegewsation cand the second. part ef Bil was-Sa Teacher Sat ce mn a f: a ete oo Cartin [onornn ou 1 __|byected te wWhasonication G RESOLT. Thas we produced invertase containing Yeast cols .