gs Camtin [ras
1
I
mal
j (| GRAPE FERMENTATION FOR THE PRODUCTION OF
pa : a RED WINE
LAM:
| "To produce wine Loy, p grape fomentation and to estimate
i J alcohel_content.
3
[| PRINCIPLE:
| Wine is the end product ef. complete o¢ bacteria) alcoho
fermentation of qrape 61. othen Puuits by -the -ackion Of yeait]
| : eq! :
| cells + Wine cells produced when ajeact Kall erry matically
Jade guibSugan fauctese dnd qbcose finst bo Sidelyde”
Lhem +e aloha » Grapes contain 20-30% Cf Suqan will yield
i wine with alcohol courtent approximately 5H.
tune for feunentaction is about ao—286 amd_fenmentation is
Lannied Suk im pen oA closed vessel.
MATERIALS REQUIRED
ed qxapes cheese cloth » 4 east cells.
lo-1S % + The-Lempens
PROCE DURE-
Ui) tke 6£ grapes wee weighed and thoroughly, washed ond
Tevashed’ withcalars red. Od
| i) aog ef yeast was thoyoughly dissolved in boiled and
ieee ca
Gi) Crushed qaupes ,sugan and yeast wore Aayered Oven se
venal tines to gel ach ef conical alas.
(iv) Le was Cotton“ plugg ed amd Gmelloated at room tempera
tune te Helease oa
(wy) Six weeks Rater, wine was extracked using cheese cloth
land Aoid eaten was discanded:
| DETERMINATION OF AlcoHoL CONTENT
BG mb ef wine was -taken .and Lemtnguged at _Loooypny
Teaches Snaive
warren WT.
ce rena Aexperiment Name / No:
Carlin [root
cami eeaal
__|efer tomin Abou Amt ef. Supernatemt was-taken in atest tu
be wasted ag +test_Usmt of distilled water, 6:25 ml of chr.
wewic acid -and_o:S ml ep 40% Na-k -aulfendte was added te
the tesk-tubes. Then the -besk Lubes weye incubated at 90%
L4oy 15 min Approximate amount ef aleohel_, cevnicadd and
40% Na-K -tantenate acid distilled waten was taken in blank
and om @then 5 test tubes. The oD Values wene-taken ata
| wave length of Goonm. A hh was Pl tked with Conconbra-|
“ion ou x ale amd oD Value On ynaris » From this qpaphas
desho|_contevit was found eu:
RESULT
Wine was produced by 1. fementation