1 Alpha-lipoic acid ameliorates sodium valproate-induced liver injury in mice

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3 Chrismawan Ardianto1, Hijrawati Ayu Wardani1, Nurrahmi1, Mahardian Rahmadi1, Junaidi Khotib1
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5 1. Department of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60286,
6 Indonesia
7 Corresponding author: Chrismawan Ardianto, e-mail: chrismawan-a@ff.unair.ac.id
8 Co-authors: HAW: hijrawatiayuwardani@gmail.com, N: nurrahmi.siswanto20@gmail.com, MR:
9 mahardianr@ff.unair.ac.id, JK: junaidi-k@ff.unair.ac.id
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11 Abstract
12Aim: The aim of this study was conducted to examine the effect of α-lipoic acid (ALA) on sodium
13valproate-induced liver injury observed by histological feature of the liver tissue of mice.
14Materials and Methods: Mice were divided into three groups: (1) vehicle group, (2) sodium valproate
15group, (3) sodium valproate-ALA group. Vehicle group was injected with saline i.p. for 28 days. Sodium
16valproate group was injected with sodium valproate 300 mg/kg i.p. daily for 14 days, then proceed with
17vehicle every day until day 28. The sodium valproate-ALA group was injected with sodium valproate 300
18mg/kg daily for 14 days prior to the intraperitoneal administration of ALA 100 mg/kg until day 28. Mice
19were sacrificed and the liver was extracted for histopathological examination.
20Results: Histopathological examination of the liver section of vehicle group showed the normal structure
21of liver. Two weeks after the administration of sodium valproate, histopathological examination showed
22an abnormal structure of liver, which contains necrosis appearance and inflammatory cells. Treatment
23with ALA after the induction of sodium valproate notably ameliorated the hepatic histopathological
24lesions and performed a similar structure to the normal liver.
25Conclusion: ALA ameliorates sodium valproate-induced liver injury in mice.
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27Keywords: α-lipoic acid, DILI, histopathological, liver injury, sodium valproate