Section II
Prepared Tubed, Bottled Media, cont.
Gram staining include staining reagents, immersion oil, glass
slides and the specimens used for inoculation. If there is
uncertainty about the validity of the Gram stain, the culture
should be reincubated for another hour or two and the test
repeated before a report is given.
30
Difco Manual Sect II.ind 30
XIII. References
1. U.S. Public Health Service, Centers for Disease Control and Prevention, and National Institutes of
Health. 2007. Biosafety in microbiological and biomedical laboratories, 5th ed. HHS Publication
No. (CDC) 93-8395. U.S. Government Printing Office, Washington, D.C.
2. Clinical and Laboratory Standards Institute. 2004. Approved standard: M22-A3, Quality control for
commercially prepared microbiological culture media, 3rd ed. CLSI, Wayne, Pa.
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