CHLORIDE

(Mercuric Thiocyanate Method)

For the determination of Chloride in serum
(For In vitro Diagnostic Use Only)

SUMMARY:

Chloride, a major anion, is important in the maintenance of the cation /anion balance between intra and extra-

cellular fluids. This electrolyte is therefore essential to the control proper hydration, osmotic pressure and acid/base

equilibrium. Elevated serum chloride values may be seen in dehydration, hyperventilation, congestive heart valve

and prostatic or other types of urinary obstruction. Low serum chloride values are found with extensive burns,

excessive vomiting, intestinal obstruction, nephritis, metabolic acidosis, and in Addisonian crisis.

PRINCIPLE:

The Chloride ions react with mercuric thiocyanate to release thiocyanate ions, which in turn react with ferric ions to

form a red colored complex of ferric thiocyanate. The intensity of the color is proportional to the chloride

concentration.

2Cl- + Hg (SCN)2 HgCl2 +2 (SCN)-

3(SCN)- + Fe3+ Fe(SCN)3 (red colored complex)

CONTENTS:

PACK SIZE MTC REAGENT(A1) STANDARD (S)

1x50ml 1x50ml 5ml

STABILITY AND STORAGE:

It is stable at room temperature up to the date of expiration as specified, avoid direct sunlight

REAGENT PREPARATION:

Reagents are ready to use.

SAMPLE MATERIAL:

Serum, heparinised plasma, urine and CSF.

GENERAL SYSTEM PARAMETERS:

Reaction Mode End point Sample Volume 10 µl

Wavelength 505 nm Reagent volume 1000 µl
Blank Reagent blank Standard 100 mmol/L
Incubation 37˚C Reaction Slope Increasing
Delay Time 5 Sec Linearity 150 mmol/L
Read Time 5 Sec Units mmol/L

ASSAY PROCEDURE:

Pipette into clean dry test tubes labelled as blank (B), standard (S) and Test (T) :

Addition Sequence B(µl) S(µl) T(µl)

MTC Reagent(A1) 1000 1000 1000

Standard(S) - 10 -

Sample - - 10

Mix well read absorbance of standard (S) and Test (T) against Reagent blank (B) at 505nm
CALCULATIONS:
Abs. of Test
Chloride (mmol/L) = ———————— x 100
Abs.of Standard
mEq/l. = mmol/L

NORMAL REFERENCE VALUES:

Serum : 98 - 107 mEq/l (98-107 mmol/l)

CSF : 123 - 128 mEq/l (123-128 mmol/L)
Urine : 170 - 250 mmol/24h.

LINEARITY:

This method is linear up to 150 mmol/L

REFERENCE

1. Schoenfeld RG, Lerveller CV.Clin Chem 10,533 (1964)

2. Levinson,S.S (1976) Clin Chem,22,273